Sakineh Kavyanifar; Tayebeh Shamspur; Fariba Fathirad; Ali Mostafavi
Abstract
Objective: The purpose of this work was design and performance investigation of a nanocarrier based on magnetic nanofibers containing core-shell nanostructuresfor anticancerdrug delivery of daunorubicin (DAN) by measuring their drug release at different pH values.
Methods: Fe3O4 nanoparticles ...
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Objective: The purpose of this work was design and performance investigation of a nanocarrier based on magnetic nanofibers containing core-shell nanostructuresfor anticancerdrug delivery of daunorubicin (DAN) by measuring their drug release at different pH values.
Methods: Fe3O4 nanoparticles and Fe3O4@SiO2core-shell nanostructures were synthesized through coprecipitation and Stöber methodrespectively. The composite nanofibers of polyvinyl alcohol containing core-shell nanostructures and anticancer drug of daunorubicinwere fabricated by electrospinning method.The nanostructures were characterized bySEM, XRD,VSM and FTIR techniques. The drug release was investigated by UV-Vis spectrophotometer at different pHs.
Results: The results is shown that in vitro drug release at pH= 6.0 is promisingly more and faster than drug release at pH= 7.4. The fitted equation of release curves is corresponded to Peppas model.
Conclusions: It can be concluded that the proposed nanocarrier is capable of responding to pH changes, that is an advantage in the targeted delivery of the drug. Also, this method has the advantages of magnetic sensitivity, high drug loading capacity and sustained release.
Tayebeh Shamspur; Fariba Fathirad; Mitra Ghanbari; Saeed Esmaeili Mahani
Abstract
Objective(s): The purpose of this study was preparation and evaluation of PVA-Fe3O4 nanofibers as nanocarrier of doxorubicin (DOX) by measuring their drug release together with their in vitro cytotoxicity toward cancer cells at different pH values. Methods: Fe3O4 nanoparticles were synthesized by coprecipitation ...
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Objective(s): The purpose of this study was preparation and evaluation of PVA-Fe3O4 nanofibers as nanocarrier of doxorubicin (DOX) by measuring their drug release together with their in vitro cytotoxicity toward cancer cells at different pH values. Methods: Fe3O4 nanoparticles were synthesized by coprecipitation method. The composite nanofibers of polyvinyl alcohol containing nanoparticles and anticancer drug DOX were fabricated by electrospinning method. The nanostructures were characterized by different techniques. The drug release was investigated by UV-Vis spectrophotometer at different pHs and 37.5 ̊C. Results: In vitro drug release experiments show that the doxorubicin release at pH= 6.0 is promisingly more and faster than drug release at pH= 7.4. The fitted equation of release curves corresponds to Peppas model. Also, MTT assays indicate that the MNPs-doxorubicin-loaded nanocarrier has cytotoxicity comparable with free drug. Conclusions: The synthesized nanocarrier was successfully used for the efficient delivery of an anti-cancer drug into the tumor region. The DOX-loaded nanocarrier showed a steady and sustained release profile in vitro up to 72 h. The drug release from nanocarrier was better described using Peppas model.
Fatemeh Mehrabi; Tayebeh Shamspur; Ali Mostafavi; Asma Saljooqi; Fariba Fathirad
Abstract
Objective(s): The purpose of this study was to compare novel sandwich-structured nanofibrous membranes, and coaxial and usual methods, to provide sustained-release delivery of morphine for drug delivery. In this work, synthesis ofnanofibrous cellulose acetate (NFC) was carried out by electrospinning. ...
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Objective(s): The purpose of this study was to compare novel sandwich-structured nanofibrous membranes, and coaxial and usual methods, to provide sustained-release delivery of morphine for drug delivery. In this work, synthesis ofnanofibrous cellulose acetate (NFC) was carried out by electrospinning. Methods: A weighed amount of cellulose acetate (CA) powder was dissolved in 3:1 v/v acetone/dimethylformamide (DMF) to obtain a CA solution at a concentration of 8 to16% w/v. Acetaminophen or morphine-loaded CA solutions were prepared by dissolving CA powder and Acetaminophen (A) or morphine in the weight ratio of 5:1, in an acetone/DMF mixture. Under optimum condition, they were electrospun into sandwich structured membranes with the coaxial method and cellulose acetate as the surface layer and cellulose acetate/drugs as the core. Results: Characterization of the radius of fiber is shown as 52.9 ± 0.1nm with scanning electron microscopy (SEM). The full range drug release profiles of nanofibers are shown as 80.7% of the contained drug in 8h. The drug release from nanofiber was controlled through a typical Fickian diffusion mechanism from the cellulose acetate matrix by a release exponent value of 0.24 for conventional nanofiber, 0.35 for coaxial nanofiber and 0.40 (less than 0.45) for sandwich nanofibers. Conclusions: All the cellulose acetate nanofibers showed that they could release large amounts of drugs in vitro for more than one day. However, among these three methods, the best one is a sandwich method because its release is slower than that of the other methods.
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