Ali alirezaie alavijeh; Milad Rajabi; Farid Barati; Moosa Javdani; Iraj Karimi; Mohammad Barati; Mohsen Moradian
Abstract
Catgut was enriched with copper sulfate nanoparticles (CSNPs@Catgut), in order to develop a new composited suture with antibacterial and healing properties. Introducing copper sulfate nanoparticles to catgut was performed using a reverse micro-emulsion technique. It is an interesting method because of ...
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Catgut was enriched with copper sulfate nanoparticles (CSNPs@Catgut), in order to develop a new composited suture with antibacterial and healing properties. Introducing copper sulfate nanoparticles to catgut was performed using a reverse micro-emulsion technique. It is an interesting method because of easy handling and relatively low costs. In the revers micro-emulsion medium, nano-spherical structures containing the salt solution are created. The nano-spheres penetrate into catgut fibers and precipitate after drying to form the salt nanoparticles. The prepared CSNPs@Catgut was characterized using scanning electron microscopy, X-ray diffraction (XDR) technique, tensile strength, antibacterial activity, and cytotoxicity tests. XRD and SEM confirmed the CuSO4 nanoparticles formation and grafting on catgut surface. Antibacterial properties were illustrated by E. coli inhibition zone and CSNPs@Catgut showed a significant antibacterial activity compare with catgut. Results of cytotoxicity tests showed no difference between CSNPs@Catgut and catgut. The mechanical strength was improved and increased from 4.16 mm to 10.00 mm. Tissue reaction showed that necrosis and inflammatory cells were reduced from acute to subacute on day 14. Therefore, CSNPs@Catgut can be introduced as a reliable candidate for wound management.
Ali Alirezaie Alavijeh; Masoomeh Dadpey; Farid Barati; Mohammad Barati
Abstract
Objective(s): In the developed countries, one of the leading causes of death is cancer. Cancer cells with their unique and destructive characteristics impose high costs on communities.
Methods: In this study, we will review the latest cancer researches and diagnostic tools.
Results: Existing ...
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Objective(s): In the developed countries, one of the leading causes of death is cancer. Cancer cells with their unique and destructive characteristics impose high costs on communities.
Methods: In this study, we will review the latest cancer researches and diagnostic tools.
Results: Existing technologies, using clinical markers and studying the polymeric screeners of cancer cells, have created kits that make cancer diagnosis at early stages. The study of the survival mechanism in malaria and honey bee and the development of bacterial engineering has created new approaches to create powerful tools for cancer detection and control.
Conclusions: The completion of these processes will create the potential for cancer eradication. Study of the survival mechanisms in nature and purposive modeling of it will lead to provide many solutions to solve the problems. These studies can lead to develop accurate diagnostic and therapeutic tools.
Ali Alirezaie Alavijeh; Masoomeh Dadpey; Mohammad Barati; Afsaneh Molamirzaie
Abstract
Objective(s): The objective of the current study was to prevent surgical site infection (SSI) by creating a new antibacterial silk suture.
Methods: Cefixime trihydrate (CFX) was prepared as nanoparticles via mixing with polyvinyl alcohol (PVA) hydrogel by covalent cross-linkage. The mixture was stirred ...
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Objective(s): The objective of the current study was to prevent surgical site infection (SSI) by creating a new antibacterial silk suture.
Methods: Cefixime trihydrate (CFX) was prepared as nanoparticles via mixing with polyvinyl alcohol (PVA) hydrogel by covalent cross-linkage. The mixture was stirred vigorously to obtain a homogenous gel. Under this condition the polymer chains separate CFX as nanoparticles and trap them (CFX@PVA). The enrichment of silk suture was performed by immersing it in the CFX@PVA solution. The trapped CFX nanoparticles in PVA hydrogel on the surface of sutures were confirmed by SEM. The effect of CFX@PVA silk sutures on tensile strength was analyzed, using a Santammachine controller. The antibacterial activity of the reinforced silk suture was tested on E. coli (ATCC25922) and S. aureus (ATCC25924).
Results: All antibacterial studies clearly showed that the use of novel CFX@PVA silk sutures could represent clinical advantages, in terms of prevention of resistant bacteria, such as Staphylococcus aureus (S. aureus), the same as the sensitive bacteria, for 15 days. The maximum elongation of composite before rupture, modulus and extension, showed statistically significant difference between reinforced silk sutures and untreated silk suture. No statistically significant difference was found between the Failure load, Stress, Bending and Energy.
Conclusions: Our data indicate that CFX@ Silk sutures are capable of reducing the risk of SSIs, and has a good mechanical strength to keep the wound sides closed, during early healing recovery.
