nanomedicine
Rajaa H Salih; Wajeeh Kachi Obead; Ahmed Majeed Al-Shammari
Abstract
Objective(s): This study aimed to prepare a stable colloidal silver nanoparticles (AgNPs) and assess its novelty combination therapy, which comprises AgNPs and lives attenuated Measles virus (MV) vaccine, to target breast cancer cells. The safety of the proposed therapy in normal breast epithelial cell ...
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Objective(s): This study aimed to prepare a stable colloidal silver nanoparticles (AgNPs) and assess its novelty combination therapy, which comprises AgNPs and lives attenuated Measles virus (MV) vaccine, to target breast cancer cells. The safety of the proposed therapy in normal breast epithelial cell lines (HBL-100) was evaluated.Methods: Silver nanoparticles prepared by chemical reduction, The stability, size, and concentration of the colloidal component have been demonstrated by examining the ultraviolet-visible (UV-Vis) spectroscopy at various times using Zeta potential examination, atomic force microscopy (AFM), and atomic spectroscopy. MV was propagated using the VERO-hSLAM cell line. Cytotoxicity assay was evaluated on human breast cancer cell lines. The safety of the proposed therapy in normal Human breast Luminal epithelial cells was assessed to compare the effect against cancer cell lines.Results: The formation of nanoparticles is confirmed by the appearance of a perfect surface plasmon resonance (SPR) band at 424 nm. The stability was proved via a slight change in the absorption intensity from 224 nm to 226 nm (immediately and after a month), respectively, and the value of the charge was -41.13 mV. NPs were spherical in shape and had an average diameter of 40.87 nm. The concentration was 13 µg/ml. The Chou–Talalay analysis revealed synergism between the Measles virus and silver nanoparticles in all tested cancer cell lines and there were highly significant differences (p-value<0.001) among them Conclusions: The novel combination of AgNPs and MV showed effective antitumor activity against breast cancer cells with high safety in normal human breast cells.
nanomedicine
Abolfazl Akbari; Zahra Shokati Eshkiki; Sara Mayahi; Seyed Mohammad Amini
Abstract
Curcumin is a herbal supplement that has been mentioned for many biomedical applications. Several pieces of research demonstrated that curcumin could improve cancer chemotherapy, lagging the metastasis progress, and prevent healthy cells from radiation therapy damage. Through a simple and green synthesis ...
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Curcumin is a herbal supplement that has been mentioned for many biomedical applications. Several pieces of research demonstrated that curcumin could improve cancer chemotherapy, lagging the metastasis progress, and prevent healthy cells from radiation therapy damage. Through a simple and green synthesis procedure, stable gold nanoparticles were synthesized by natural phytomedicine curcumin. The curcumin-coated gold nanoparticles (Cur@AuNPs) are red and represent the distinct gold nanoparticles' plasmonic peak. The average diameter of the synthesized nanoparticles is 21.7. However, the hydrodynamic diameter was 45.1 nm. The cytotoxicity of Cur@AuNPs has been investigated through an MTT assay. 24-hour treatment of Cur@AuNPs could eradicate more than 30% of HT29 cancerous cells. The real-time polymerase chain reaction (PCR) technique was used to study the molecular change in apoptotic protein changes. The nanoparticle treatment increases the level of the pro-apoptotic protein (Bax, P53, and P21) and decreases the anti-apoptotic protein level (Bcl-2) in the treated cell population.
Shilapa V P; Wilson Vilgeena; Bhagyasree S; E Alby Babu; Davis Nidhina; Kurup Meena; Muddukrishnaiah K
Abstract
Green synthesis of metallic nanoparticles using medicinal plant extract treated to environmentally friendly, stable, cost-effective. Present work to study In-vitro antioxidant and cytotoxicity of green synthesised CuNPs using P. Pellucida plant extract. Copper nanoparticles were synthesised biogenically ...
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Green synthesis of metallic nanoparticles using medicinal plant extract treated to environmentally friendly, stable, cost-effective. Present work to study In-vitro antioxidant and cytotoxicity of green synthesised CuNPs using P. Pellucida plant extract. Copper nanoparticles were synthesised biogenically using P. Pellucida plant aqueous extract. CuNPs Characterised by using FE-SEM, U.V., FT-IR, EDAX. Biogenically synthesised copper nanoparticles conducted in-vitro Antioxidant (DPPH) and cytotoxicity (SK-MEL-3 cell). The results CuNPs were characterised by using U.V. spectroscopy absorbance 575 nm. Scanning electronic microscope showing the distribution and shape (5-20 nm) of the nanoparticles. X-ray spectrum showed different peaks for CuNPs detected at 2θ values 35.45°, 44.32°, and 65.25°. EDAX elemental spectroscopy conformed to the copper metal inside the nanoparticle (69.7%). The synthesised CuNPs showed good free radical scavenger activity than the ascorbic acid, and it also showed significant cytotoxicity (16μg/mL) against human skin cell lines (SK-MEL-3 cell). The current study conclusion recommended that green synthesised (P. Pellucida) CuNPs be used for therapeutic application.
Shilan Nasri; Mahdi Rahaie; Bahman Ebrahimi-Hoseinzadeh; Ashrafalsadat Hatamian-Zarmi; Razi Sahraeian
Abstract
Black cumin contains biologically active compounds such as Thymoquinone, which have strong anti-cancer properties. However, most of these agents have poor stability and solubility that limits its use as drugs. In this work, an anti-cancer ethosomal nanostructure containing black cumin extract (BCE) was ...
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Black cumin contains biologically active compounds such as Thymoquinone, which have strong anti-cancer properties. However, most of these agents have poor stability and solubility that limits its use as drugs. In this work, an anti-cancer ethosomal nanostructure containing black cumin extract (BCE) was prepared to release as transdermal-controlled. After synthesis and evaluation the vesicles for size and charge, as well as determining the ratio of in vitro and ex vivo permeability, experiments to assay their cell toxicity and apoptosis were also investigated. It was confirmed the stable shape (containing 5% soy lecithin, 45% ethanol, and 1.5% cholesterol with a zeta potential of -61±2 and polydispersity index of 0.14± 0.012.), spherical morphology (20nm) and the effective release rate (40% after 24h in ex vivo permeability test) of these loaded ethosomal nanocarriers using the HPLC, DLS, FTIR, TGA methods and the in vitro and ex vivo release tests,. MTT bioassay with BCE (96µg/ml compared to 200 µg/ml) and DOX separately and their ethosomal forms showed the higher cellular toxicity of ethosomic forms on MCF-7. Flow cytometry also proved strong apoptosis in the MCF-7 cells treated with ethosomal compared to non-ethosomal forms (~64.7% for BCE (5.90% in late apoptotic stage), and ~21.6% of BCE-Eth (~71.8% in late apoptosis stage). In conclusion, our findings show that this new nanoparticle not only improves the enclosure of plant metabolites and chemotherapeutic agents but also increases the effectiveness of metabolites by increasing their controlled release and so on reduces the side effects of chemotherapy drugs.
Fatemeh Sharifi; Fariba Sharififar; Sara Soltanian; Mohsen Doostmohammadi; Neda Mohamadi
Abstract
Objective: This study aimed to investigate the cytotoxicity, antileishmanial and antibacterial potential of silver nanoparticles (AgNPs) synthesized by (Salvia officinalis L. (Sage)) extract. Methods: The green synthesized nanoparticles were analyzed by using UV–vis spectroscopy, FTIR, and FESEM ...
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Objective: This study aimed to investigate the cytotoxicity, antileishmanial and antibacterial potential of silver nanoparticles (AgNPs) synthesized by (Salvia officinalis L. (Sage)) extract. Methods: The green synthesized nanoparticles were analyzed by using UV–vis spectroscopy, FTIR, and FESEM techniques. Cytotoxicity antileishmanial assay was evaluated by MTT. Results: The UV–vis spectroscopy study at the band of 430 nm confirmed the formation of nanoparticles. FT-IR confirmed the presence of the ingredients in salvia leaf extract which is responsible for capping and reduction of the silver nanoparticles. FESEM report showed that the AgNPs synthesized were in the size range 30–70 nm. The AgNPs exhibited good cytotoxic activity against Hek-293, the breast cancer cell line (MCF 7) and a human glioblastoma cell line (A 172) with an IC50 value of 240, 50.40 and 58.60 µg/mL, respectively. The present study confirmed good antileishmanial activity against the promastigote and amastigote stages of Leishmania major in a dose-dependent manner. IC50 values of AgNPs were 62.91 and 73.89 μg/mL using promastigote and amastigotes assay respectively. AgNPs showed potent antibacterial activity against three bacteria species S. aureus, B. subtilis, and MRSA (Methicillin-resistant Staphylococcus aureus) at high concentrations. According to disk diffusion results, AgNPs at concentration 1000 µg/mL showed a significant inhibitory zone against S. aureus and B. subtilis. The AgNPs at a concentration higher than 750 µg/mL showed the valuable inhibitory zone against MRSA. Conclusion: this study confirmed good cytotoxicity, and antileishmanial activity in the nanoparticles synthesized using S. officinal extract, so it can be further investigated for biomedical applications.
Sudipta Panja; Anutosh Patra; Kalyani Khanra; Indranil Choudhuri; Bikash Ranjan Pati; Nandan Bhattacharyya
Abstract
The objective of the study is to synthesis silver nanoparticles from the leaf extract of Cryptolepis buchanani, a medically important plant,by green synthesis method. The synthesized nanoparticles have an average size of 17.05±5.27 nm, crystalline in nature with face cantered cubic structure, ...
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The objective of the study is to synthesis silver nanoparticles from the leaf extract of Cryptolepis buchanani, a medically important plant,by green synthesis method. The synthesized nanoparticles have an average size of 17.05±5.27 nm, crystalline in nature with face cantered cubic structure, and positive surface charge. The nanoparticles are biologically active. It killed > 90 % of HeLa cells at 25 μg mL-1 concentration in-vitro cell cytotoxicity assay, with a LD50 value of 3.98 μg mL-1. The nanoparticles are less effective on MCF-7 and HEK-293 cell line, almost 70 % of MCF-7 populations were survived at highest concentration of 25 μg mL-1. In case of HEK-293 it killed almost 78 % of cells at the same concentration with a 9.45 μg mL-1 LD50 value. The synthesized nanoparticle lysed less than 2.5 % of red blood cells at 10 μg mL-1 concentration in-vitro. The nanoparticles degraded >90 % of methylene blue dye in presence of light in 8.5 h. These properties of the synthesized nanoparticles are unique, and make it promising for its future potential applications.
Shilpa V P; Samuel Thavamani B; Roshni E.R; Sangeetha Vijayan U; Lekshmi MS Panicker; Bhagyasree S; Jilsha G; Muddukrishnaiah K
Abstract
< p>The Aim of this study is to examine the in vitro antibacterial and cytotoxicity activities of green synthesized Zinc Oxide (ZnO) nanoparticles using leaf aqueous extract of Allamanda cathartica (L). Zinc nitrate acts as a precursor and aqueous leaf extract chemical reducing agent. Green synthesized ...
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< p>The Aim of this study is to examine the in vitro antibacterial and cytotoxicity activities of green synthesized Zinc Oxide (ZnO) nanoparticles using leaf aqueous extract of Allamanda cathartica (L). Zinc nitrate acts as a precursor and aqueous leaf extract chemical reducing agent. Green synthesized ZnO nanoparticles are verified by an analysis of the powder X-ray diffraction. The FTIR study indicates the presence of different functional groups in the leaf extract as well as in the ZnO nanoparticles. The UV absorption wavelength was measured at a high of 360 nm. The crystalline size, shape and surface morphology of ZnO nanoparticles are measured using Field Emission Scanning Electron Microscopy (FE-SEM). The EDAX spectrum conform that zinc and oxygen were present in the ZnO nanoparticles. Green synthesized ZnO nanoparticles exhibited attractive antibacterial activity against clinical bacillus sp. The anticancer activity of ZnO nanoparticles is also tested against Human breast cancer MCF7 cell and were significantly reduced the proliferation of MCF7 cell comparison with the control cell viability.
Shirin Tavakoli; Fatemeh Sameni; Mohammad Ali Ebrahimzadeh; Pourya Biparva; Hamidreza Mohammadi; Afshin Zahedi; Alireza Rafiei; Mostafa Kardan; Shahram Eslami
Abstract
Objective(s): Nowadays, examining the toxicity of nanoparticles including the synthesized and functionalized iron nanoparticles using methods like green synthesis is highly considered, due to their increasing usage in various fields of medicine, biology, industrial, and pollution removal. Hence, in this ...
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Objective(s): Nowadays, examining the toxicity of nanoparticles including the synthesized and functionalized iron nanoparticles using methods like green synthesis is highly considered, due to their increasing usage in various fields of medicine, biology, industrial, and pollution removal. Hence, in this study, the toxicity of the zero valent iron nanoparticles synthesized by plant-Myrtus communis (MC-ZVINP) was investigated. Methods: Human normal Foreskin Fibroblast (HFF) cells were used for cytotoxicity examination using MTT method. Also, biochemical factors such as liver enzymes level, and factors such as the number of white and red globules, lymphocytes, platelets, amount of blood hemoglobin, and histopathological test of liver tissue in laboratory small rats were examined after intraperitoneal injections of the MC-ZVINP with different concentrations daily and a duration of 3-month, with the groups receiving trivalent iron, the extract of plant-case, and normal saline. Results: Cytotoxicity concentration of iron-case nanoparticles was obtained for 50% of HFF cells (CC50=149.23±4.45μg/mL). The results obtained from the blood factors examination showed a decreased the serum level of liver enzymes as well as an increase in the number of red and white globules and hemoglobin rate in mice receiving iron nanoparticles compared to the trivalent iron receiving group. Receiving the concentrations of 100 and 200 mg/kg/bw of iron nanoparticles have caused the incidence of mild and moderate inflammation in the liver of mice. Conclusions: Generally, it can be concluded that, the MC-ZVINP have shown no significant toxicity on the levels of blood cells, enzymes, and liver tissue.
Samira Ghorbani; Hamdollah Moshtaghi; Seyed Shahram Shekarforoush; Hamid Reza Ghaisari; Fatemeh Sedaghati; Maryam abbasvali
Abstract
The use of nanoparticles (NPs) has been extended to many fields such as agriculture, food industry, medicine and biotechnological fields. Thereby, human exposure to NPs consequently increases. Therefore, there is a concern about the potential biological effects and toxicity of NPs for humans and the ...
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The use of nanoparticles (NPs) has been extended to many fields such as agriculture, food industry, medicine and biotechnological fields. Thereby, human exposure to NPs consequently increases. Therefore, there is a concern about the potential biological effects and toxicity of NPs for humans and the environment. This study aimed to investigate the cytotoxicity effects of magnesium oxide (MgO) and silica (SiO2) NPs on human colon adenocarcinoma (HT-29) after 24 hours of exposure. In this study, cytotoxicity of MgO and SiO2 NPs was evaluated using MTT assay after 24 hours of MgO (50 nm) and SiO2 (90-110 nm) NPs exposure at doses of 25-200 µg/ml. Moreover, to assess the rate of cell apoptosis, cells were stained with ethidium bromide/acridine orange stain. The staining was examined under a fluorescent microscope. The exposure of HT29 cells to SiO2 and MgO NPs increased cytotoxicity in a dose-dependent manner. Also, these results showed a significant increase in apoptosis induction in treated groups with MgO and SiO2 NPs. These results showed that SiO2 and MgO NPs can cause cytotoxicity in HT29 cells and it is better to avoid using them in food stuff and food packaging ingredients.
Abbas Rahdar; Mousa Aliahmad; Yahya Azizi; Nasser Keikha; Mahdiyeh Moudi; Farshid Keshavarzi
Abstract
Objective(s): In this work, CuO- NiO nano-composites were synthesized via free-surfactant co-precipitation method and then their physiochemical properties, as well as cytotoxicity and antifungal effects, were studied. Methods: The structural and optical properties of CuO-NiO nanostructures ...
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Objective(s): In this work, CuO- NiO nano-composites were synthesized via free-surfactant co-precipitation method and then their physiochemical properties, as well as cytotoxicity and antifungal effects, were studied. Methods: The structural and optical properties of CuO-NiO nanostructures were analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM), Atomic force microscope (AFM), UV–Vis absorption, and vibrating sample magnetometer (VSM) techniques. MTT assay was used to evaluate the cytotoxicity of nanostructures. Results: The cubical structure of CuO- NiO nano-composites was confirmed by the XRD technique. The optical study of the samples by UV-Vis indicted a blue shift in absorption wavelength with decreasing particle size due to quantum size effect. The super magnetic behavior of CuO-NiO nano composites after calcination was confirmed by magnetic characterization instrument. Finally, the results of cytotoxicity evaluation of CuO-NiO nano-composites at the lower concentrations on Breast cancer MDA cell lines demonstrate no significant toxicity. Minimum inhibitory concentration range and Minimum fungicidal concentration of nanoparticle were determined 0.97-15.62, 7.81µg/ml and for fluconazole were 1.75-25 µg/ml and 12.58 µg/ml, respectively. Conclusions: The study result of antimicrobialof CuO-NiO nano composites indicated an MIC90 antifungal activity with a concentration of 3.90µg/ml against vaginal isolates of C. albicans. The results of cytotoxicity study of nano-composites at concentration of 50µg/ml and 10µg/ml on the cell line of Breast cancer MDA was equivalent to %60 and %80, respectively.
Neamat Karimi; Shahab Bohlooli; Mohammad Mazani
Abstract
Objective(s): The aim of this study was to determine cytotoxic effect of nanoliposomal form of lyophilized aqueous extract of Ecballium elaterium fruit on gastric cell line (AGS) using cell viability tests. Methods: An aqueous extract of the fruits of Ecballium elaterium was prepared. Nanoliposomal form ...
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Objective(s): The aim of this study was to determine cytotoxic effect of nanoliposomal form of lyophilized aqueous extract of Ecballium elaterium fruit on gastric cell line (AGS) using cell viability tests. Methods: An aqueous extract of the fruits of Ecballium elaterium was prepared. Nanoliposomal form was also prepared by thin-film hydration method and stability size was determined by SEM. The zeta potential and size characterized by Malvern zetasizer. Cytotoxic effect of the nanoliposomes encapsulated the extract on cell line was examined by MTT, Neutral Red and Frame methods. Results: The size of nanoliposomes was 218.2 nm with proper dispersion (PDI=0.3). The morphology of the liposomes was suitable according to SEM image. The IC50 values indicated that the nanoliposomal form of extract was 2-3 times more active than extract alone. The average IC50 values for extract and nanoliposomal form of extract were 1±0.1 and 0.39±0.02 μg/ml, respectively. Conclusions: The results from this study showed that the crude extract and nanoliposomal form extract of Ecballium elaterium have cytotoxicity effect on AGS cell line and these cells were significantly more susceptible to nanoliposomes encapsulated Ecballium elaterium extract than that of the extract itself.
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