Wagner. J. Fávaro; Renata A., Villela; Helder J. Ceragioli; Nelson Duran
Abstract
Raman spectroscopy is a promising diagnostic technique used to identify different cancer types; however, few reports have correlated this information to histopathological analyses conducted in vivo or ex-vivo. It is well-known that using a set of techniques is important and necessary to get reliable ...
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Raman spectroscopy is a promising diagnostic technique used to identify different cancer types; however, few reports have correlated this information to histopathological analyses conducted in vivo or ex-vivo. It is well-known that using a set of techniques is important and necessary to get reliable and safer results. Identifying chemical changes in the Raman spectrum of healthy and pathological tissues enables better understanding the effects of treatments to be adopted, as well as optimizing pathological information and preventing cell death from taking place as slow biomolecule degradation of biomolecules. The treatment applied to non-muscle invasive bladder cancer (NMIBC) in the presence of reduced graphene oxide (rGO), rGO with cisplatin, rGO with doxorubicin, as well as the association of chemotherapeutics, such as rGO, with cisplatin and doxorubicin, followed by Raman spectroscopy and histopathological analyses, have shown the feasibility of using these two techniques to monitor NIMBC development in rats based on different chemotherapeutic formulations. Moreover, Raman tests have confirmed structural and biochemical changes in urinary bladder due to pathological process and exposure to chemotherapeutic agents.
Fatemeh Gheybi; Seyedeh Hoda Alavizadeh; Seyed Mahdi Rezayat; Elham Zendedel; Mahmoud Jaafari
Abstract
Cancer is the second leading cause of death worldwide. Despite great efforts over many years, today cancer treatment is not very effective. The main reasons for cancer chemotherapy failure are high cytotoxicity, low response rates in solid tumors, and development of resistance. Different experimental ...
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Cancer is the second leading cause of death worldwide. Despite great efforts over many years, today cancer treatment is not very effective. The main reasons for cancer chemotherapy failure are high cytotoxicity, low response rates in solid tumors, and development of resistance. Different experimental studies have shown that drug combination using low toxicity natural compounds such as polyphenols can reduce the required dose of cytotoxic drugs for cancer treatment. The polyphenolic compound, Silymarin (SLM), is an active extract from the seeds of the plant milk thistle (Silybum Marianum). It is well known for its hepatoprotective, antioxidant and chemoprotective effects. In present study, we investigated cytotoxic effects of combined liposomal doxorubicin (DXL) and SLM on 4T1 breast cancer cells at different molar ratios of the two drugs and we focused on elucidating whether the combination of the two drugs could dictate antitumor activity in vitro. Results indicated that SLM-DXL combination at 100 and 300 molar ratios, exert synergistic growth-inhibitory effects. These synergistic effects were observed only at lower SLM-DXL concentrations. In conclusion, it is conceivable that in SLM-DXL combination chemotherapy, drug ratios play a key role which determine the final response following treatment. Thus, using liposomes as targeted drug delivery systems, it would be possible to achieve appropriate combination of the two drugs at correct doses and correct administration intervals clinically. Keywords: Silymarin; Doxorubicin; Liposomes; Combination therapy; 4T1
Tayebeh Shamspur; Fariba Fathirad; Mitra Ghanbari; Saeed Esmaeili Mahani
Abstract
Objective(s): The purpose of this study was preparation and evaluation of PVA-Fe3O4 nanofibers as nanocarrier of doxorubicin (DOX) by measuring their drug release together with their in vitro cytotoxicity toward cancer cells at different pH values. Methods: Fe3O4 nanoparticles were synthesized by coprecipitation ...
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Objective(s): The purpose of this study was preparation and evaluation of PVA-Fe3O4 nanofibers as nanocarrier of doxorubicin (DOX) by measuring their drug release together with their in vitro cytotoxicity toward cancer cells at different pH values. Methods: Fe3O4 nanoparticles were synthesized by coprecipitation method. The composite nanofibers of polyvinyl alcohol containing nanoparticles and anticancer drug DOX were fabricated by electrospinning method. The nanostructures were characterized by different techniques. The drug release was investigated by UV-Vis spectrophotometer at different pHs and 37.5 ̊C. Results: In vitro drug release experiments show that the doxorubicin release at pH= 6.0 is promisingly more and faster than drug release at pH= 7.4. The fitted equation of release curves corresponds to Peppas model. Also, MTT assays indicate that the MNPs-doxorubicin-loaded nanocarrier has cytotoxicity comparable with free drug. Conclusions: The synthesized nanocarrier was successfully used for the efficient delivery of an anti-cancer drug into the tumor region. The DOX-loaded nanocarrier showed a steady and sustained release profile in vitro up to 72 h. The drug release from nanocarrier was better described using Peppas model.
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