Shilan Nasri; Mahdi Rahaie; Bahman Ebrahimi-Hoseinzadeh; Ashrafalsadat Hatamian-Zarmi; Razi Sahraeian
Abstract
Black cumin contains biologically active compounds such as Thymoquinone, which have strong anti-cancer properties. However, most of these agents have poor stability and solubility that limits its use as drugs. In this work, an anti-cancer ethosomal nanostructure containing black cumin extract (BCE) was ...
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Black cumin contains biologically active compounds such as Thymoquinone, which have strong anti-cancer properties. However, most of these agents have poor stability and solubility that limits its use as drugs. In this work, an anti-cancer ethosomal nanostructure containing black cumin extract (BCE) was prepared to release as transdermal-controlled. After synthesis and evaluation the vesicles for size and charge, as well as determining the ratio of in vitro and ex vivo permeability, experiments to assay their cell toxicity and apoptosis were also investigated. It was confirmed the stable shape (containing 5% soy lecithin, 45% ethanol, and 1.5% cholesterol with a zeta potential of -61±2 and polydispersity index of 0.14± 0.012.), spherical morphology (20nm) and the effective release rate (40% after 24h in ex vivo permeability test) of these loaded ethosomal nanocarriers using the HPLC, DLS, FTIR, TGA methods and the in vitro and ex vivo release tests,. MTT bioassay with BCE (96µg/ml compared to 200 µg/ml) and DOX separately and their ethosomal forms showed the higher cellular toxicity of ethosomic forms on MCF-7. Flow cytometry also proved strong apoptosis in the MCF-7 cells treated with ethosomal compared to non-ethosomal forms (~64.7% for BCE (5.90% in late apoptotic stage), and ~21.6% of BCE-Eth (~71.8% in late apoptosis stage). In conclusion, our findings show that this new nanoparticle not only improves the enclosure of plant metabolites and chemotherapeutic agents but also increases the effectiveness of metabolites by increasing their controlled release and so on reduces the side effects of chemotherapy drugs.
Tayebeh Shamspur; Fariba Fathirad; Mitra Ghanbari; Saeed Esmaeili Mahani
Abstract
Objective(s): The purpose of this study was preparation and evaluation of PVA-Fe3O4 nanofibers as nanocarrier of doxorubicin (DOX) by measuring their drug release together with their in vitro cytotoxicity toward cancer cells at different pH values. Methods: Fe3O4 nanoparticles were synthesized by coprecipitation ...
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Objective(s): The purpose of this study was preparation and evaluation of PVA-Fe3O4 nanofibers as nanocarrier of doxorubicin (DOX) by measuring their drug release together with their in vitro cytotoxicity toward cancer cells at different pH values. Methods: Fe3O4 nanoparticles were synthesized by coprecipitation method. The composite nanofibers of polyvinyl alcohol containing nanoparticles and anticancer drug DOX were fabricated by electrospinning method. The nanostructures were characterized by different techniques. The drug release was investigated by UV-Vis spectrophotometer at different pHs and 37.5 ̊C. Results: In vitro drug release experiments show that the doxorubicin release at pH= 6.0 is promisingly more and faster than drug release at pH= 7.4. The fitted equation of release curves corresponds to Peppas model. Also, MTT assays indicate that the MNPs-doxorubicin-loaded nanocarrier has cytotoxicity comparable with free drug. Conclusions: The synthesized nanocarrier was successfully used for the efficient delivery of an anti-cancer drug into the tumor region. The DOX-loaded nanocarrier showed a steady and sustained release profile in vitro up to 72 h. The drug release from nanocarrier was better described using Peppas model.
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