Elham Salami; Manizheh Karami; Ameneh Jafaryan Dehkordi; Mohammadreza Jalali Nadoushan; Abazar Hajnorouzi
Abstract
Background and Objective: Morphine can cause harmful effects in the ovaries. The silver nanoparticles (Ag-NPs) used in health care because of antimicrobial properties, can diffuse into the brain blood barrier. This study investigated the protective effect of Ag-NPs on the induction of polycystic ovary ...
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Background and Objective: Morphine can cause harmful effects in the ovaries. The silver nanoparticles (Ag-NPs) used in health care because of antimicrobial properties, can diffuse into the brain blood barrier. This study investigated the protective effect of Ag-NPs on the induction of polycystic ovary (PCO) due to injection of morphine intra-ventromedial hypothalamus (intra-VMH) of rat compared with the animal receiving the drug (10-100 mg/kg) intraperitoneally (i.p.) twice daily for 7 days to lead to addiction. Materials and Methods: The rats (bought of Pasture Institute of Iran weighing 200-250 g) were housed under standard conditions and fed ad libitum. They were randomly divided to addict to drug or morphine (0.001 to 0.4 μg/rat) receiving into the VMH (AP: -1.92) a week after stereotaxic surgery. Ag-Nps (0.01, 0.001 and 0.0001 μg/rat) were administered intra-VMH alone or prior to morphine effective dose (0.4 μg/rat). Control group was given only saline. By the end of the treatment, the animals’ ovaries and/or brain were dissected and studied histopathologically. The ovaries were also checked by the marker of the NO, NADPH-diaphorase. Results: All experimental rats’ ovaries treated morphine showed polycystic characteristics and the NO activation was evidenced in the ovaries in the comparison with the saline group (p
Ehsan Zayerzadeh; Meisam Shabanian; Mohammad Kazem Koohi
Abstract
Objective(s): Due to wide range of medical applications as bactericidal agents, nanosilver particles (NSPs) are manufactured worldwide in large quantities. However, potential toxicity impacts of NSPs in humans and animals still remain poorly understood. The objective of this study was to investigate ...
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Objective(s): Due to wide range of medical applications as bactericidal agents, nanosilver particles (NSPs) are manufactured worldwide in large quantities. However, potential toxicity impacts of NSPs in humans and animals still remain poorly understood. The objective of this study was to investigate clinical observations, mortality and pathological changes in rats following intraperitoneal administration of different doses of NSPs.
Methods: In this study, rats were administered intraperitoneally over a period of 5 days with repeated doses at different dose levels (20, 80, 320 mg/kg) of NSPs (20 nm). Rats were euthanized 14 days after the treatment. Animal mortality, clinical sings, food intake and body weight were evaluated. Histopathology was performed on heart, lung, liver and kidneys of experimented animals.
Results: There was a significant decrease in the body weight of animals in high dose group following fourteen days of exposure. Also, there was significant decrease in food intake during the treatment period in high dose group. Histological tissue sections indicated that NSPs induced multi-organ pathological lesions including severe alveolar edema, hemorrhage and inflammation in lungs, myocytolysis, congestion and edema in heart, inflammation and congestion in kidney and liver.
Conclusions: The results obtained two weeks following intraperitoneal injection indicate that the administration of high doses of NSPs could induce histopathological complications in heart, lung, liver and kidneys in rats. No significant pathological effects were observed in low and intermediate doses. More toxicological investigations are needed in relation of the application of NSPs with their potential threat as a medical tool.
