Shirin Tavakoli; Mohammad Ali Ebrahimzadeh; Fatemeh Sameni; Pourya Biparva; Hamidreza Mohammadi; Ali Ziar; Afshin Zahedi Mazandarani; Soheil Vafaeinejad; Shahram Eslami
Abstract
Objective(s): Nowadays, iron ions intoxication is the center of attention of interest in the management and treatment of thalassemia and different sorts of anemia associated with regular blood transfusions. Due to the major side effects of current drugs, they should be replaced with safer alternatives. ...
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Objective(s): Nowadays, iron ions intoxication is the center of attention of interest in the management and treatment of thalassemia and different sorts of anemia associated with regular blood transfusions. Due to the major side effects of current drugs, they should be replaced with safer alternatives. Thus, in this study, functionalized hybrid silver nanoparticles, as an emerging perspective, were investigated for absorbing excess iron ions and their removal in an animal thalassemia model. Methods: The silver nanoparticles were green-synthesized using the Myrtus communis leaf methanolic extact (MC-AgNPs). The produced hybrid nano-Sorbents based on hydrolyzable tannin matrix loaded with silver nanoparticles were delivered for trying out in vivo iron chelation in thalassemia model mice in which iron-overload was imposed. MC-AgNPs and desferral were injected intraperitoneally four times a week for one month in mice with excess iron load. The total iron and Fe3+ content of serum was evaluated with the aid of plasma-atomic spectrometry microscopy and a Fe3+ ion measurement kit, respectively. Also, liver enzyme levels were evaluated by an auto-analyzer. Also, hepatic enzyme levels were appraised by using an auto-analyzer based the corresponding kits. Morphological transformations of the liver tissue were investigated by way of Prussian blue staining. Results: The mice treated with the MC-AgNPs demonstrated a significant reduction in serum iron content when compared with the iron-overload mice. MC-AgNPs revealed satisfying effectiveness to chelate excess iron in mice. Conclusions: This method could be considered as a competitive option for lowering the level of excess iron in vivo.
Shirin Tavakoli; Mohammad Ali Ebrahimzadeh; Emran Habibi; Pourya Biparva; Hamidreza Mohammadi; Afshin Zahedi Mazandarani; Soheil Vafaeinejad; Ali Ziar; Shahram Eslami
Abstract
Objective(s): The use of silver nanoparticles in the field of biomedicine is increasing day by day, but less attention has been paid to its toxicity. In this paper, the ability of the silver nanoparticles produced by a green synthesis procedure to protect the liver and its effects on liver function in ...
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Objective(s): The use of silver nanoparticles in the field of biomedicine is increasing day by day, but less attention has been paid to its toxicity. In this paper, the ability of the silver nanoparticles produced by a green synthesis procedure to protect the liver and its effects on liver function in male mice was investigated in a sub-chronic toxicity study. Methods: The silver nanoparticles functionalized the hydrolyzable tannin fraction of Myrtus communis (MC-AgNPs) were used for testing in vivo sub-chronic toxicity in mice model. The MC-AgNPs and Ag+ were intraperitoneally injected with different doses 5 times a week over 90 days. The biochemical, hematological factors were determined using an autoanalyzer following the routine procedures. In addition, histopathological test of liver tissue in laboratory mice were examined through haematoxylin & eosin staining. Results: The obtained results showed that liver enzymes (AST, ALT, and ALP) were decreased. The mean value ± standard deviation of white blood cells, lymphocytes, red blood cells and Hb were increased, while red blood cells and hemoglobin decreased. Histopathological investigations indicated no obvious effect on hepatic cyto-architecture in the group receiving silver nanoparticles (50 mg/kg), and mild inflammation in the port space. In the groups receiving silver nanoparticles (100 and 200 mg/kg), mild inflammation, and moderate inflammation were observed in the port space and pre portal, respectively. Conclusions: The findings indicated that AgNPs could be safe even for long-term use in a therapeutic period if hybridized with active biomolecules.
Shirin Tavakoli; Fatemeh Sameni; Mohammad Ali Ebrahimzadeh; Pourya Biparva; Hamidreza Mohammadi; Afshin Zahedi; Alireza Rafiei; Mostafa Kardan; Shahram Eslami
Abstract
Objective(s): Nowadays, examining the toxicity of nanoparticles including the synthesized and functionalized iron nanoparticles using methods like green synthesis is highly considered, due to their increasing usage in various fields of medicine, biology, industrial, and pollution removal. Hence, in this ...
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Objective(s): Nowadays, examining the toxicity of nanoparticles including the synthesized and functionalized iron nanoparticles using methods like green synthesis is highly considered, due to their increasing usage in various fields of medicine, biology, industrial, and pollution removal. Hence, in this study, the toxicity of the zero valent iron nanoparticles synthesized by plant-Myrtus communis (MC-ZVINP) was investigated. Methods: Human normal Foreskin Fibroblast (HFF) cells were used for cytotoxicity examination using MTT method. Also, biochemical factors such as liver enzymes level, and factors such as the number of white and red globules, lymphocytes, platelets, amount of blood hemoglobin, and histopathological test of liver tissue in laboratory small rats were examined after intraperitoneal injections of the MC-ZVINP with different concentrations daily and a duration of 3-month, with the groups receiving trivalent iron, the extract of plant-case, and normal saline. Results: Cytotoxicity concentration of iron-case nanoparticles was obtained for 50% of HFF cells (CC50=149.23±4.45μg/mL). The results obtained from the blood factors examination showed a decreased the serum level of liver enzymes as well as an increase in the number of red and white globules and hemoglobin rate in mice receiving iron nanoparticles compared to the trivalent iron receiving group. Receiving the concentrations of 100 and 200 mg/kg/bw of iron nanoparticles have caused the incidence of mild and moderate inflammation in the liver of mice. Conclusions: Generally, it can be concluded that, the MC-ZVINP have shown no significant toxicity on the levels of blood cells, enzymes, and liver tissue.
