Arezoo Jammanesh; Sepideh Arbabi Bidgoli; Solmaz Ghaffari; Mohammad Reza Avadi
Abstract
Aims and Objectives: We aimed in present study to provide solid-lipid nanoparticle (SLN) of GBE and evaluate its oral safety in the light of recent discoveries on strong inhibitory effects of Ginkgo Biloba Leaf Extract (GBE) against Covid 19 and Influenza virus .Materials and Methods: Morphology and ...
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Aims and Objectives: We aimed in present study to provide solid-lipid nanoparticle (SLN) of GBE and evaluate its oral safety in the light of recent discoveries on strong inhibitory effects of Ginkgo Biloba Leaf Extract (GBE) against Covid 19 and Influenza virus .Materials and Methods: Morphology and particle sizes of nanoparticles were analyzed by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and Dynamic Light Scattering (DLS) methods. In the next step, the release profile of 85% loaded SLNs was determined by dialysis membrane method. Acute and repeated dose oral toxicity tests were performed on selected freeze-dried formulation with appropriate stability in female mice according to OECD 425 and OECD 407 guidelines .Results: The spherical GBE-SLN released GBE during the first 72 hrs. In acute oral test, doses up to 2000 mg/kg didn’t cause mortality or any sign of toxicity .Repeated dose oral toxicity study on three dose levels (0.5, 5, 50 mg/kg/ day), didn’t show any abnormal change due to clinical, biochemical and necropsy evaluations but hematological assessment showed abnormal coagulation in accordance with some abnormal changes in the liver, kidney, heart, lungs and ovaries of high dose (50mg/kg) group of animals. Conclusion: Repeated dose oral administration of GBE-SLN in doses up to 5 mg/kg/day was considered as safe dose level. Later studies are mandatory for evaluating the preventive and therapeutic effects of present GBE-SLN against Covid 19 in appropriate in vitro and in vivo models.
Pegah Haghighi; Solmaz Ghaffari; Sepideh Arbabi Bidgoli; Mahnaz Qomi; Setareh Haghighat
Abstract
Objective(s): In this work, Ginkgo biloba extract (GBE) loaded solid lipid nanoparticles (SLNs) were synthesized via high pressure homogenization method and their physicochemical properties, as well as cytotoxicity and antibacterial activities were evaluated.Methods: Ginkgo biloba extract SLNs (GBE-SLNs) ...
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Objective(s): In this work, Ginkgo biloba extract (GBE) loaded solid lipid nanoparticles (SLNs) were synthesized via high pressure homogenization method and their physicochemical properties, as well as cytotoxicity and antibacterial activities were evaluated.Methods: Ginkgo biloba extract SLNs (GBE-SLNs) were prepared using high pressure homogenization method. The morphology and size of SLNs were evaluated by scanning electron microscopy (SEM) and dynamic light scattering (DLS) techniques. The drug release of SLNs was also investigated using synthetic dialysis membrane. The antibacterial activity of nanoparticles was tested against both gram negative and gram positive bacteria strains. The probability of having toxicity of SLNs was studied on the rabbits.Results: The spherical structure of GBE-SLNs was confirmed by SEM images. The mean particle size of the obtained SLNs was ranging from 104 to 621 nm for different formulations using DLS technique. An in-vitro study of synthesized SLNs illustrated that the percentage of ginkgo biloba released from the solid lipid nanoparticles was 85% of loaded GBE after 72 hours. There was no report of significant skin toxicity via in-vivo studies.Conclusions: According to the above results, SLNs loaded with ginkgo extract showed acceptable particle size and shape, suitable loading of active substance and sustained release profile as well as appropriate antimicrobial effects without any significant skin toxicity.
