nanomedicine
Roghayeh Norouzi; Sakineh Irani; Farzad Katiraee; Marzie Hejazy
Abstract
Objective(s): The Hyalomma species of ticks is hematophagous ectoparasites and responsible for transmission of protozoan, bacterial, rickettsial and viral infection in vertebrate animals and humans. Recently, nanobiotechnology is considered as a novel approach for the control of ticks. The aim of this ...
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Objective(s): The Hyalomma species of ticks is hematophagous ectoparasites and responsible for transmission of protozoan, bacterial, rickettsial and viral infection in vertebrate animals and humans. Recently, nanobiotechnology is considered as a novel approach for the control of ticks. The aim of this study is to evaluate acaricidal activity of nanocristobalite (nano-SiO2) against the Hyalomma spp. in vitro and to determine its toxicity by MTT assay.Methods: The acaricidal activities of the SiO2-NPs were evaluated at concentrations of 50, 125 and 250 mg/ml and controls (distilled water and Cypermethrin) at exposure times of 10, 30 and 60 min, were performed each with two replications and two spraying and contact methods. Then the toxicity of each concentration was evaluated by MTT assay. Mortality percentages and LC50 values were calculated. Data were analysed by Graph Pad Prism 5 software.Results: The results of this study revealed all concentrations of SiO2-NPs had acaricidal activity and concentration of 250 mg/ml of SiO2-NPs had the highest acaricidal effect (90%) at 60 min exposure time. The results showed that the spray method was more effective than the contact method and the results of MTT toxicity test showed that nano-SiO2 have very little toxicity in all concentrations. The median lethal concentration (LC50) values were obtained at 125 mg/ml in 60 min. Conclusions: The results indicated that SiO2-NPs contained potent acaricidal activity and might provide new acaricidal compounds for the effective control of Hyalomma spp. However, further investigations are needed to determine the efficacy of SiO2-NPs in vivo.
Roghayeh Norouzi; Amin Ataei; Marzie Hejazy; Parisa Shahbazi
Abstract
Objective(s): Hyalomma spp. is responsible for transmission of protozoan, bacterial, rickettsial and viral diseases in humans and animals. Recently, there was a wide number of promising attempts to evaluate and use of nanoparticles for the control of ticks. Methods: The aim of this study was to evaluate ...
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Objective(s): Hyalomma spp. is responsible for transmission of protozoan, bacterial, rickettsial and viral diseases in humans and animals. Recently, there was a wide number of promising attempts to evaluate and use of nanoparticles for the control of ticks. Methods: The aim of this study was to evaluate the acaricidal activity of zinc oxide nanoparticles (ZnO NPs) size 15 nm against Hyalomma spp. in vitro. The arcaricidal activity of Zn NPs were evaluated at concentrations of 50, 125 and 250 mg/ml and controls (distilled water and Cypermethrin) following 10, 30 and 60 min of exposure in triplicate and the experiments were performed two spraying and contact methods. Results: The results of this study showed that all concentrations of Zn NPs had acaricidal activity and concentration of 125 mg/ml at exposure time of 30 min and concentration of 250 mg/ml at all exposure times had highest acaricidal effect (100%). The median lethal concentration (LC50) values were 50 mg/ml in 60 min and (LC99) values were 150 mg/ml in 30 min for Hyalomma spp.. The results showed that the spray method was more effective than the contact method. Conclusions: The findings of present study showed that Zn NPs had potent acaricidal effect and recommended as an effective acaricidal agent. However, further in vivo studies are required to evaluate the efficacy of this nanoparticle.
