Hamed Mahmoudi; Pooya Jafari Doudaran
Abstract
Objective(s): Extracellular Vesicles (EVs) transports active biomolecules between human many cells. As natural nanoparticles (NP), exosomes (Exo), plays important roles in the diagnosis and also treatment, of human disorders. Recently, it has been exhibited that mesenchymal stem cell (MSC)-derived Exo ...
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Objective(s): Extracellular Vesicles (EVs) transports active biomolecules between human many cells. As natural nanoparticles (NP), exosomes (Exo), plays important roles in the diagnosis and also treatment, of human disorders. Recently, it has been exhibited that mesenchymal stem cell (MSC)-derived Exo could regulate immune cells' biological processes. We evaluated the influence of the MSCs-derived Exo on T cell survival.Methods: Exo were procured from the supernatant of bone marrow (BM)-MSCs. The structure and form of MSC-Exo were investigated using transmission electron microscopy (TEM). Exo markers, CD9 and CD63, were detected by Western blotting. In co-culture condition with MSC-derived Exo, the survivals of T cells were assessed by flow cytometry. Also, the expression levels of the Caspase-3, Caspase-8, Bcl-2 and Mcl-1 were measured by Real-time PCR.Results: MSCs-derived Exo induced apoptosis in human T cells more strongly within 72 hours but not 24 hours of co-culture. Real-time PCR results also showed that Exo co-culture led to down-regulation of Mcl-1, and Bcl-2 while inducing Caspase 3 and 8 expressions at mRNA levels. Conclusions: MSCs-derived Exo could induce apoptosis in T cells and lead to immunomodulation by triggering caspase cascade and also down-regulating anti-apoptotic protein levels.
Farzaneh Chehelcheraghi
Abstract
Background and objective: In the present paper, impacts of gold Nanoparticles (AUNPs) and Chick Embryo Extract Therapy (CEET) were investigated on the wound healing promoting properties that match the dynamic healing process of skin flap regeneration.Methods: AUNPs were developed using the high-intensity ...
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Background and objective: In the present paper, impacts of gold Nanoparticles (AUNPs) and Chick Embryo Extract Therapy (CEET) were investigated on the wound healing promoting properties that match the dynamic healing process of skin flap regeneration.Methods: AUNPs were developed using the high-intensity short-pulse beams mode of neodymium-doped: Yttrium, Aluminum, and Garnet photoablation method. Also, 45-µg extract from a 4-6 day chick embryo was used as a protein source for CEET. Dorsal Random Skin Flaps (RSFs) were created in forty-five Albino Wistar rats and they were accidentally assigned to 3 groups; G(C); group did not receive any treatment (control), G (AUNP); treated only with AUNPs, and G(CE+AUNP) the rats treated with AUNPs and CEET. During a random skin flap animal model, AuNPs and CE were used as solutions and convenient to deliver into the skin flap. The skin flap survival rate was evaluated in the following by a camcorder, and tissue studies were done at days 3, 6, and 9 after surgery by the hematoxylin & eosin and Masson trichrome staining.Result: Rats treated with the AUNPs and CEET exhibited remarkably increased flap survival than the others. Tissue studies showed that AUNPs and CEET caused induction of forming the new blood vessels and stimulated the inflammation-causing reaction in an initial phase of wound healing. Conclusion: Using AUNPs with CEET increases the potential for a dynamic recovery of skin flap regeneration.
