Masoumeh Keshavarz; Navid Ahmadi Nasab; Maryam Saeidifar; Sara Nikoofal; Omid Safa
Abstract
Objective(s): Curcumin (Cur) as a natural bioactive compound has shown potential capability to fight a variety of malignancies. In this study, bovine serum albumin nanoparticles (BSA NPs) were applied to improve bioavailability and increase the effectiveness of the hydrophobic curcumin against human ...
Read More
Objective(s): Curcumin (Cur) as a natural bioactive compound has shown potential capability to fight a variety of malignancies. In this study, bovine serum albumin nanoparticles (BSA NPs) were applied to improve bioavailability and increase the effectiveness of the hydrophobic curcumin against human glioblastoma brain cancer cells.Methods: BSA NPs were synthesized and Cur was loaded in nanoparticles, based on desolvation method. Characterization studies were performed using dynamic light scattering, UV-Visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy (TEM) and differential thermal analysis (DTA). Curcumin release from albumin nanoparticles was investigated in vitro and finally the cytotoxicity evaluation against U-87 MG cell line was studied by MTT method.Results: The curcumin loaded nanoparticles (BSA-Cur NPs) showed a homogeneous spherical shape with mean particle size and polydispersity index (PDI) of 182.1 ± 2.02 nm and 0.105 ± 0.02, also drug loading content (DL%) and encapsulation efficiency (EE%) were obtained 11.73% and 83.26%, respectively. Cur showed a sustained release from BSA NPs with maximum release percentage of 30% after 48 hours. The results of MTT assay revealed that after 48 h treatment BSA-Cur NPs have more cytotoxicity on U-87 MG cells compared to free Cur, owning IC50 values of 33.08 ± 0.1.27 and 17.43 ± 1.37, respectively.Conclusions: According to the results of this study, albumin nanoparticles can be considered as a promising carrier for improving the effectiveness of curcumin in drug delivery against glioblastoma.
Hadis Kheradjoo; Alireza Nouralishahi; Mohamad Saeid Hoseinzade Firozabdi; Saman Mohammadzadehsaliani
Abstract
Aims: Based on the literature, mesenchymal stem/stromal cell derived exosome has dual role in tumor therapy or progress. We evaluated the possible anti-tumor effect of the MSCs-exosome on retinoblastoma Y-79 cell cells in vitro.Methods: To verify the efficient isolation of exosome, the MSCs-exosome structure ...
Read More
Aims: Based on the literature, mesenchymal stem/stromal cell derived exosome has dual role in tumor therapy or progress. We evaluated the possible anti-tumor effect of the MSCs-exosome on retinoblastoma Y-79 cell cells in vitro.Methods: To verify the efficient isolation of exosome, the MSCs-exosome structure and the expression of CD9 and CD81 was examined using transmission electron microscopy (TEM) and western blotting, respectively. Y-79 cell proliferation and apoptosis were evaluated using MTT assay and flow cytometry, respectively. The expression levels of Bcl-2 and microRNA-143 were estimated by the Real-time PCR.Results: MSCs-exosome inhibited Y-79 cells proliferation as shown by MTT assay results. Also, MSCs-exosome induced apoptosis in Y-79 cells within 72 hours of treatment. Real-time PCR results also exhibited that exosome therapy resulted in a decrease in Bcl-2 expression while up-regulating microRNA-143 expression.Conclusion: MSCs-derived exosome could deter retinoblastoma Y-79 cells tumorigenicity, thereby providing novel modality to manage retinoblastoma patients.
Ghazaleh Rahimi; Maryam Saeidifar; Monireh Ganjali; Esmaeil Salahi
Abstract
One of the new strategies for improving of chemotherapy in cancer treatment is the use of nanocarriers for sustained release of anticancer drugs. The present study aims to investigate the bovine serum albumin nanoparticles (BSANP) with exosomes (Exo) for prolonged release of 5-Flurouracil (5FU) as an ...
Read More
One of the new strategies for improving of chemotherapy in cancer treatment is the use of nanocarriers for sustained release of anticancer drugs. The present study aims to investigate the bovine serum albumin nanoparticles (BSANP) with exosomes (Exo) for prolonged release of 5-Flurouracil (5FU) as an anticancer drug model. 5FU with Exo was loaded to BSANP (5FU.Exo@BSANP) and the system was characterized by FTIR, AFM and FESEM. Furthermore, 5FU.Exo and 5FU@BSANP were prepared and characterized to compare their release behavior with that of 5FU.Exo@BSANP. The binding properties examined via FTIR confirmed the formation of the above-mentioned systems. FESEM analysis of BSANP and 5FU.Exo@BSANP showed the spherical morphology with the average particle size 313±60 nm and 403±64 nm, respectively, while, 5FU.Exo had a cylindrical morphology with average particle size in width 200±36 nm. AFM results demonstrated the reduction of roughness in 5FU.Exo@BSANP. In addition, the release behavior indicated that sustained release of 5FU occurred when it was loaded to nanocarriers. However, the release of 5FU from 5FU.Exo@BSANP at pH 7.4 was slower than in the other systems. Furthermore, the kinetic model of all systems was followed by Korsmeyer-peppas with Fickian diffusion while 5FU.Exo@BSANP at pH 5.5 was zero order kinetic model. Moreover, MTT assay onto 4T1 cancer cell lines explored the significant cytotoxicity of 5FU.Exo@BSANP. Thus, the designed nanocarrier of Exo@BSANP is a promising system for sustained release of 5FU.
Fatemeh Gheybi; Seyedeh Hoda Alavizadeh; Seyed Mahdi Rezayat; Elham Zendedel; Mahmoud Jaafari
Abstract
Cancer is the second leading cause of death worldwide. Despite great efforts over many years, today cancer treatment is not very effective. The main reasons for cancer chemotherapy failure are high cytotoxicity, low response rates in solid tumors, and development of resistance. Different experimental ...
Read More
Cancer is the second leading cause of death worldwide. Despite great efforts over many years, today cancer treatment is not very effective. The main reasons for cancer chemotherapy failure are high cytotoxicity, low response rates in solid tumors, and development of resistance. Different experimental studies have shown that drug combination using low toxicity natural compounds such as polyphenols can reduce the required dose of cytotoxic drugs for cancer treatment. The polyphenolic compound, Silymarin (SLM), is an active extract from the seeds of the plant milk thistle (Silybum Marianum). It is well known for its hepatoprotective, antioxidant and chemoprotective effects. In present study, we investigated cytotoxic effects of combined liposomal doxorubicin (DXL) and SLM on 4T1 breast cancer cells at different molar ratios of the two drugs and we focused on elucidating whether the combination of the two drugs could dictate antitumor activity in vitro. Results indicated that SLM-DXL combination at 100 and 300 molar ratios, exert synergistic growth-inhibitory effects. These synergistic effects were observed only at lower SLM-DXL concentrations. In conclusion, it is conceivable that in SLM-DXL combination chemotherapy, drug ratios play a key role which determine the final response following treatment. Thus, using liposomes as targeted drug delivery systems, it would be possible to achieve appropriate combination of the two drugs at correct doses and correct administration intervals clinically. Keywords: Silymarin; Doxorubicin; Liposomes; Combination therapy; 4T1
Pouya Dehghankelishadi; Farid Dorkoosh
Abstract
Pluronic based nano-particulate systems are innovative platforms for delivery of anti-cancer agents. These systems due to their pharmacological properties and suitable physicochemical characteristics are great opportunity for development cancer therapeutics. This mini-review tries to provide a more detailed ...
Read More
Pluronic based nano-particulate systems are innovative platforms for delivery of anti-cancer agents. These systems due to their pharmacological properties and suitable physicochemical characteristics are great opportunity for development cancer therapeutics. This mini-review tries to provide a more detailed overview on the currently available pluronic based drug delivery systems. In the section 2, pharmacological characteristics of pluronic as a therapeutic polymer are assessed. In section 3, pluronic based formulations for undruggable payloads and surfaced modified targeted delivery systems are analyzed. The combinatorial pluronic based systems are summarized in section 4 and at last but not least the challenges and future prospective are discussed within section 5.
Neda Soleimani; Masoumeh Tavakoli-Yaraki; Baharak Farhangi
Abstract
Objective(s): Breast cancer imposes a highest rate of malignancy among the women all around the world. Chitin and its derivatives such as D-glucosamine-carboxymethyl chitin and Di-hydroxy propyl chitin have immune-modulating effects and influence on innate and acquisitive immunity which lead to cell ...
Read More
Objective(s): Breast cancer imposes a highest rate of malignancy among the women all around the world. Chitin and its derivatives such as D-glucosamine-carboxymethyl chitin and Di-hydroxy propyl chitin have immune-modulating effects and influence on innate and acquisitive immunity which lead to cell activity enhancement. The aim of this study was investigating the effect of D-glucosamine nanoparticle on immune responses such as the changes in cytokines type 1 and 2 level in tumoral mice. Methods: Nanoparticles were synthesized by ionic gelation method and characterized by DLS and SEM methods. Tumors were induced in experimental mice and subsequently treated with nanoparticles. Then, the production of cytokine interferon-γ (IFN-γ) and interleukin 4 (IL-4) were evaluated. Results: The obtained results showed a significant increase in the level of IFN-γ production in the mice group treated with nanoparticles compared to control groups. Additionally, there was a reduction in the level of IL-4 and tumor size in the test group. Conclusions: D-glucosamine nanoparticles can be proposed as a stimulator of the immune system and a promising compound for cancer treatment in the future.
