nanomedicine
Rajaa H Salih; Wajeeh Kachi Obead; Ahmed Majeed Al-Shammari
Abstract
Objective(s): This study aimed to prepare a stable colloidal silver nanoparticles (AgNPs) and assess its novelty combination therapy, which comprises AgNPs and lives attenuated Measles virus (MV) vaccine, to target breast cancer cells. The safety of the proposed therapy in normal breast epithelial cell ...
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Objective(s): This study aimed to prepare a stable colloidal silver nanoparticles (AgNPs) and assess its novelty combination therapy, which comprises AgNPs and lives attenuated Measles virus (MV) vaccine, to target breast cancer cells. The safety of the proposed therapy in normal breast epithelial cell lines (HBL-100) was evaluated.Methods: Silver nanoparticles prepared by chemical reduction, The stability, size, and concentration of the colloidal component have been demonstrated by examining the ultraviolet-visible (UV-Vis) spectroscopy at various times using Zeta potential examination, atomic force microscopy (AFM), and atomic spectroscopy. MV was propagated using the VERO-hSLAM cell line. Cytotoxicity assay was evaluated on human breast cancer cell lines. The safety of the proposed therapy in normal Human breast Luminal epithelial cells was assessed to compare the effect against cancer cell lines.Results: The formation of nanoparticles is confirmed by the appearance of a perfect surface plasmon resonance (SPR) band at 424 nm. The stability was proved via a slight change in the absorption intensity from 224 nm to 226 nm (immediately and after a month), respectively, and the value of the charge was -41.13 mV. NPs were spherical in shape and had an average diameter of 40.87 nm. The concentration was 13 µg/ml. The Chou–Talalay analysis revealed synergism between the Measles virus and silver nanoparticles in all tested cancer cell lines and there were highly significant differences (p-value<0.001) among them Conclusions: The novel combination of AgNPs and MV showed effective antitumor activity against breast cancer cells with high safety in normal human breast cells.
Elham Mohammadi; Seyed Meysam Amini; Seyed Hossein Mostafavi; Seyed Mohammad Amini
Abstract
With emerging drug resistance microorganism, the search for a new biocidal agent has begun. The silver nanoparticle is a synthetic material with potent antimicrobial activity that applies to a diverse library of microorganisms. But toxicity and safety concerns of chemically prepared silver nanoparticles ...
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With emerging drug resistance microorganism, the search for a new biocidal agent has begun. The silver nanoparticle is a synthetic material with potent antimicrobial activity that applies to a diverse library of microorganisms. But toxicity and safety concerns of chemically prepared silver nanoparticles toward human and environment limited the extensive industrial biomedical application of silver nanoparticles. On the other hand, curcumin is a natural phenolic compound of the Indian spice turmeric that contains mild antimicrobial activity against various microorganisms. However, instability, poor absorption and low solubility of curcumin prevent its wide application in biomedical researches. Simultaneous application of these two materials is the subject of the provided manuscript. Curcumin formulation and silver nanoparticles can be applied separately or together, but the state of the art is applying curcumin for the synthesis of silver nanoparticles that represent a better biocidal activity and lower cytotoxicity in comparison to chemically synthesized silver nanoparticles.
nanomedicine
Seyed Mohammad Amini; Maryam Samareh Salavati Pour; Reza Vahidi; Seyedeh Mehrnaz Kouhbananinejad; Mahla Sattarzadeh Bardsiri; Alireza Farsinejad; Mohamad Javad Mirzaei-Parsa
Abstract
Abstract: The therapeutic effect of silver nanoparticles on leukaemia cancerous cells has been demonstrated in several studies. However, most of these nanoparticles are toxic to normal cells as well as cancerous cells. In the present study, green chemistry has been applied for the synthesis of silver ...
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Abstract: The therapeutic effect of silver nanoparticles on leukaemia cancerous cells has been demonstrated in several studies. However, most of these nanoparticles are toxic to normal cells as well as cancerous cells. In the present study, green chemistry has been applied for the synthesis of silver nanoparticles by Teucrium polium (T.P) extract. The synthesized nanoparticles were spherical with an average diameter of 14.3 ± 9.7 nm and a surface charge of -0.84 mV. Based on the FTIR results, the silver nanoparticles have been coated with T.P extract phytochemicals. The extract was not toxic toward cancerous cells. However, the T.P extract coated silver nanoparticles (T.P@AgNPs) with concentrations ≥ 50 µg/mL could eradicate the NALM-6 cancerous cells in a significant amount. Based on the flow cytometry analysis, the predominant mechanism of cancerous cell death is the apoptosis in NALM-6 cancerous cells; and the T.P@AgNPs had no toxic effect on normal PBMC cells.
Anand Kumar Keshari; Atul Srivastava; Sandip Chowdhury; Ragini Srivastava
Abstract
AbstractObjectiveThe present work shows the green synthesis of silver nanoparticles using C. roseus extract and its antioxidant, free radicals scavenging and antibacterial activities. Methods The C. roseus extract synthesized silver nanoparticles (CrAgNPs) were characterized by X-ray diffractometry, ...
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AbstractObjectiveThe present work shows the green synthesis of silver nanoparticles using C. roseus extract and its antioxidant, free radicals scavenging and antibacterial activities. Methods The C. roseus extract synthesized silver nanoparticles (CrAgNPs) were characterized by X-ray diffractometry, Scanning Electron Microscopy, Transmission Electron Microscopy and Fourier Transform Infra Red spectroscopy. The antioxidant, hydrogen peroxide scavenging, hydroxyl radicals scavenging, superoxide scavenging and reducing power activity of CrAgNPs were determined by DPPH, hydrogen peroxide scavenging, hydroxyl radicals scavenging, superoxide scavenging and reducing power assay methods. The antibacterial activity of CrAgNPs was analyzed by Agar dilution, Minimum Inhibitory Concentration methods. ResultsThe CrAgNPs were synthesized by C. roseus extract and silvernitrate. The synthesis of silver nanoparticles was confirmed by color changes and UV-visible spectrophotometer analysis. The CrAgNPs were crystalline, variable size, elemental and spherical shape. The C. roseus extract and CrAgNPs have antioxidant, hydrogen peroxide scavenging, hydroxyl radicals scavenging, superoxide scavenging and reducing power activity. The zone of inhibition and MIC value of CrAgNPs confirmed the antibacterial activity. The CrAgNPs have greater antibacterial activity than C. roseus extract against the S. Typhi and P. vulgaris. The MIC results of CrAgNPs confirmed that CrAgNPs was highly effective against the S. Typhi and P. vulgaris bacteria. Conclusion Phenols and flavonoids of C. roseus extract reduced the silvernitrate into silver nanoparticles. The CrAgNPs were crystalline, spherical shape, variable particles size and elemental. The C. roseus extract and CrAgNPs have antioxidant, hydrogen peroxide scavenging, hydroxyl radicals scavenging, superoxide scavenging, reducing power activity and antibacterial activity.
Mahnaz Rahimpour; Manizheh Karami; Ali Haeri Rohani
Abstract
AbstractBackground: Silver nanoparticles (Ag-NPs) that are used daily in care service can enter the body and create free radicals. Despite the toxicity at high concentrations, these particles are non-toxic and useful at low concentrations. Thus, we investigated the effectiveness of nontoxic Ag-NPs to ...
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AbstractBackground: Silver nanoparticles (Ag-NPs) that are used daily in care service can enter the body and create free radicals. Despite the toxicity at high concentrations, these particles are non-toxic and useful at low concentrations. Thus, we investigated the effectiveness of nontoxic Ag-NPs to interfere with the aversive effect of naloxone (NLX) and low expression of c-Fos during testing of morphine-induced conditioned place preference (CPP) in rats. Methods: The Wistar rats (weighing 300-350 g) were cannulated bilaterally by stereotaxic apparatus for the CeA (AP= –2.12 mm; L= ±4.1 mm; V= 7.8 mm). CPP was conducted via a three-phase unbiased procedure. Morphine (0.5-7.5 mg/kg) was injected subcutaneously (sc) during the conditioning phase. NLX (0.4 µg/rat) was given, intra-CeA, 10 min before the test. Ag-NPs (0.01 µg/rat) was administered prior to the antagonist. The control group received saline (1 µL/rat, intra-CeA). c-Fos expression was quantified immunohistochemically in rats subsequent the injections: Results: The CeA and hippocampal cornu ammonis 1 (CA1) of rats that treated by NLX showed low c-Fos protein levels during testing, whereas levels of protein were high in the brains of morphine conditioned rats. Interestingly, both areas (CeA and CA1) showed similar increases in protein levels when the injection of NLX was combined with the Ag-NPs. However, these regions were not significantly different in the single Ag-NPs receiving and control groups. Conclusions: This indicates that the two regions interact with each other when NLX is injected and that in presence of Ag-NPs the protein levels are elevated in the regions.
Fatemeh Sharifi; Fariba Sharififar; Sara Soltanian; Mohsen Doostmohammadi; Neda Mohamadi
Abstract
Objective: This study aimed to investigate the cytotoxicity, antileishmanial and antibacterial potential of silver nanoparticles (AgNPs) synthesized by (Salvia officinalis L. (Sage)) extract. Methods: The green synthesized nanoparticles were analyzed by using UV–vis spectroscopy, FTIR, and FESEM ...
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Objective: This study aimed to investigate the cytotoxicity, antileishmanial and antibacterial potential of silver nanoparticles (AgNPs) synthesized by (Salvia officinalis L. (Sage)) extract. Methods: The green synthesized nanoparticles were analyzed by using UV–vis spectroscopy, FTIR, and FESEM techniques. Cytotoxicity antileishmanial assay was evaluated by MTT. Results: The UV–vis spectroscopy study at the band of 430 nm confirmed the formation of nanoparticles. FT-IR confirmed the presence of the ingredients in salvia leaf extract which is responsible for capping and reduction of the silver nanoparticles. FESEM report showed that the AgNPs synthesized were in the size range 30–70 nm. The AgNPs exhibited good cytotoxic activity against Hek-293, the breast cancer cell line (MCF 7) and a human glioblastoma cell line (A 172) with an IC50 value of 240, 50.40 and 58.60 µg/mL, respectively. The present study confirmed good antileishmanial activity against the promastigote and amastigote stages of Leishmania major in a dose-dependent manner. IC50 values of AgNPs were 62.91 and 73.89 μg/mL using promastigote and amastigotes assay respectively. AgNPs showed potent antibacterial activity against three bacteria species S. aureus, B. subtilis, and MRSA (Methicillin-resistant Staphylococcus aureus) at high concentrations. According to disk diffusion results, AgNPs at concentration 1000 µg/mL showed a significant inhibitory zone against S. aureus and B. subtilis. The AgNPs at a concentration higher than 750 µg/mL showed the valuable inhibitory zone against MRSA. Conclusion: this study confirmed good cytotoxicity, and antileishmanial activity in the nanoparticles synthesized using S. officinal extract, so it can be further investigated for biomedical applications.
Yasin Sadeghipour; Mohammad Hassan Alipour; Hamid Reza Ghaderi Jafarbeigloo; Afsaneh Salahvarzi; Mehdi Mirzaii; Ali mohammad Amani; Sareh Mosleh-shirazi; Mohsen Mehrabi
Abstract
In the present study, silver nanoparticles (Ag NPs) were synthesized by using Euphorbia Pseudocactus Berger (Euphorbiaceae) extract, which played the main role in the formation and stability of nanoparticles. The physic-chemical property of biosynthesized nanoparticles were identified using X-ray diffraction ...
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In the present study, silver nanoparticles (Ag NPs) were synthesized by using Euphorbia Pseudocactus Berger (Euphorbiaceae) extract, which played the main role in the formation and stability of nanoparticles. The physic-chemical property of biosynthesized nanoparticles were identified using X-ray diffraction (XRD), Ultraviolet–Visible spectroscopy (UV-Vis), Fourier-Transform Infrared spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM) techniques. UV-Vis results illustrated that maximum plasma resonance absorption of Ag NPs are about 426 nm. Size distribution and spherical morphology was determined by TEM method. The XRD was confirmed face centered cubic (FCC) structure for synthesized nanoparticles.The molecular dynamics (MD) and monte carlo (MC) simulations were used to evaluate the nanoparticles .The antibacterial properties of biosynthesized Ag NPs were studied on E.coli (ATCC 25922), S. aureus (ATCC 2592), P. aeruginosa (ATCC27853) and E. faecalis (ATCC51299) by using micro dilution broth method. The minimum inhibitory concentration (MIC) results of synthesized Ag NPs on S. aureus and E. faecalis obtained 4 and 8 μg/mL and P. aeruginosa and E. coli obtained 16 and 4 μg/mL. So, synthesized nanoparticles can be utilized as an antibacterial agent in medical and industrial devices and tools.
Kamrun Nahar; Deok-Chun Yang; Esrat Jahan Rupa; Mst. Khodeza Khatun; Sharif Md. Al-Reza
Abstract
Objective(s): Nanoparticles are at the leading edge of the rapidly developing field of nanotechnology. Silver nanoparticles (Ag-NPs) have received considerable attention due to their attractive physical, chemical, antimicrobial, anti-inflammatory and wound healing properties. This paper reports simple, ...
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Objective(s): Nanoparticles are at the leading edge of the rapidly developing field of nanotechnology. Silver nanoparticles (Ag-NPs) have received considerable attention due to their attractive physical, chemical, antimicrobial, anti-inflammatory and wound healing properties. This paper reports simple, cost effective and eco-friendly method for the preparation of Ag-NPs from leaf extracts of Clerodendrum viscosum using silver nitrate solution as the metal precursor. Methods: The synthesis of nanoparticles was done by using the aqueous solution of Clerodendrum viscosum leaf extract and AgNO3. The formation of nanoparticles was confirmed by the change of colour to dark brown due to the phenomenon of surface plasmon resonance. The synthesized nanoparticles were characterized by UV-Vis spectrophotometer, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Dynamic Light Scattering (DLS) and Fourier Transform Infra-red Spectroscopy (FTIR) techniques. In addition, the antibacterial activity was tested by agar disc diffusion method against some bacteria.Results: The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 422 nm. The images of the electron microscope showed that the nanoparticles were spherical and had an average size about 56 nm at 25ºC and 53 nm at 60ºC. The X-ray diffraction was clearly proven that the nanoparticles were crystalline nature. The results of microbial test also indicated that the synthesized nanoparticles had significant antibacterial activity.Conclusions: This experiment showed that leaf extracts of Clerodendrum viscosum can be used for eco-friendly synthesis of nanoparticles, which also have potential antibacterial effect.
Anand Keshari; Gaurav Pal; Samiksha Saxena; Ragini Srivastava; Vishal Srivashtav
Abstract
The study aims at synthesizing silver nanoparticles using leaf extract of Cymbopogon citratus along with the evaluation of its antioxidant, free radicals scavenging, and reducing power properties. Biosynthesized silver nanoparticles were characterized X-Ray diffractometry, Scanning Electron Microscopy, ...
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The study aims at synthesizing silver nanoparticles using leaf extract of Cymbopogon citratus along with the evaluation of its antioxidant, free radicals scavenging, and reducing power properties. Biosynthesized silver nanoparticles were characterized X-Ray diffractometry, Scanning Electron Microscopy, Transmission Electron Microscopy, Fourier Transform Infrared spectroscopy and Energy Dispersive X-ray spectroscopy. The antioxidant, free radicals and reducing power activity were determined by 2, 2-diphenyl-1-picrylhydrazyl, hydrogen peroxide scavenging, hydroxyl radicals scavenging, superoxide scavenging and reducing power activity methods. The silver nanoparticles were synthesized by Cymbopogon citratus extract that was confirmed by visible color changes of solution and spectral analysis. The biosynthesized silver nanoparticles having a surface plasmon resonance band centered at 450 nm were characterized using different techniques. The data obtained from SEM and TEM revealed the formation of spherical shape nanoparticles with size ranging from 5-35 nm in diameter while XRD suggested highly crystalline nanoparticles having Bragg’s peak at (111), (200) and (220) plane. FTIR confirmed the presence of various function groups in the extract and on the surface silver nanoparticles. The biosynthesized silver nanoparticles had greater antioxidant, free radicals scavenging and reducing power activity than Cymbopogon citratus extract while lesser activity than vitamin C.
Anand Keshari; Ragini Srivastava; Sudarshan Yadav; Gopal Nath; Surendra Gond
Abstract
ObjectiveThe present work represents the green synthesis of silver nanoparticles using Withania coagulans extract and its antibacterial property. The synergy, additive, bacteriostatic and bactericidal effect of silver nanoparticles was determined against Enterococcus faecalis, Staphylococcus aureus, ...
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ObjectiveThe present work represents the green synthesis of silver nanoparticles using Withania coagulans extract and its antibacterial property. The synergy, additive, bacteriostatic and bactericidal effect of silver nanoparticles was determined against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Salmonella typhi, and Vibrio cholerae. Methods The green silver nanoparticles were characterized by X-ray diffractometry, Transmission Electron Microscopy, Scanning Electron Microscopy and Fourier Transform Infra Red spectroscopy. The Agar dilution, Minimum Inhibitory Concentration and Bacterial Growth Inhibition methods were used for the determination of the antibacterial activity of silver nanoparticles. The Fractional Inhibitory Concentration Index method was performed to check the synergistic activity of conjugated silver nanoparticles. ResultsThe Withania coagulans extract were reduced the silver nitrate into silver nanoparticles which was confirmed by color changes and spectral analysis. The silver nanoparticles were crystalline, elemental and spherical. The antibacterial activity was reported in silver nanoparticles which confirmed by zone of inhibition and pores on the surface of bacteria. The conjugated silver nanoparticles with Levofloxacin have synergy and additive behavior against the tested bacteria. Furthermore, bacteriostatic and bactericidal nature of silver nanoparticles was reported in lower (50 µg/ml) respectively. Conclusion The phenolic compounds of W. coagulans was responsible for the formation of silver nanoparticles. The bacteriostatic and bacteriocidal activity of silver nanoparticles depends upon its concentration. The conjugation of silver nanoparticles with antibiotics may be beneficial due to its synergy and additive effect against the bacteria.
Zohreh Parang; Davood Moghadamnia
Abstract
Objective(s): Silver nanoparticles show anti-fungal properties, and is widely used in medicine. In this research, the impacts of silver nanoparticles on the hepatic functional tests and changes in liver tissues in adult male rats were investigated.
Methods: In this experimental study, 28 adult ...
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Objective(s): Silver nanoparticles show anti-fungal properties, and is widely used in medicine. In this research, the impacts of silver nanoparticles on the hepatic functional tests and changes in liver tissues in adult male rats were investigated.
Methods: In this experimental study, 28 adult male Wistar rats, each weighing approximately 180-220 g were divided into 4 groups of 7: the control group, and the experimental groups 1 and 2 received silver nanoparticles that were synthesized at 75 seconds interval with doses of 25 and 100 mg/kg intraperitoneally for 14 days, respectively. Experimental group 3 received silver nanoparticles that were synthesized at 300 seconds interval with a dose of 25 mg/kg intraperitoneally for 14 days. At the end of experiment period, blood samples were obtained from their hearts, and serum levels of hepatic enzymes (AST, ALT, ALP), albumen and total protein were measured. In addition, possible histological changes in liver was studied after hematoxylin-eosin staining. The results were statistically analyzed using ANOVA and Duncan test.
Results: The findings reported that the mean serum levels of aspartate aminotransferase (AST), total Protein and albumin in the experimental groups 1 and 3 increased significantly relative to the control group. Similarly, the mean serum levels of alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in the experimental group 3 increased significantly relative to the control group (P < 0.05). Also, necrosis of the liver tissue was observed in the recipients of silver nanoparticles.
Conclusions: The use of silver nanoparticles can boost the serum levels of hepatic enzymes and Increase liver tissue necrosis as well.
Nooshin Amini; Gholamreza Amin; Zahra Jafari Azar
Abstract
Objective(s): Nowadays, nanoparticles bio production, considering their performance in medicine and biological science, is increasing. Green synthesis of metal nanoparticles using organisms has emerged as a nontoxic and ecofriendly method for synthesis of metal nanoparticles The objectives of this study ...
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Objective(s): Nowadays, nanoparticles bio production, considering their performance in medicine and biological science, is increasing. Green synthesis of metal nanoparticles using organisms has emerged as a nontoxic and ecofriendly method for synthesis of metal nanoparticles The objectives of this study were the production of silver nanoparticles using Avena sativa L. extract and optimization of the biosynthesis process. The effects of quantity of substrate (silver nitrate (AgNo3)) and temperature on the formation of silver nanoparticles are studied. Methods: In this work, silver nanoparticles were synthesized from an extract of Avena sativa L. at different temperatures (30° C, 60° C, 90° C ) and AgNo3 concentrations( 1 mM, 2mM, 4mM) . The morphology and size of the nanoparticles were determined using Scanning Electron Microscope (SEM) and Dynamic Light Scattering (DLS). Results: SEM images showed that by increasing temperature nanoparticles size were decreased and by increasing concentrations of AgNo3 the number of nanoparticles was increased. Conclusions: The results indicated that by increasing the reaction temperature, the size of the nanoparticles would decrease. Also by increasing the concentrations of AgNo3, the amount of produced nanoparticles would be increased, but won't have a significant effect on its size. The preparation of nano- structured silver particles using Avena sativa L. extract provides an environmentally friendly option as compared to currently available chemical/ physical methods.
