Farzaneh Chehelcheraghi
Abstract
Background and objective: In the present paper, impacts of gold Nanoparticles (AUNPs) and Chick Embryo Extract Therapy (CEET) were investigated on the wound healing promoting properties that match the dynamic healing process of skin flap regeneration.Methods: AUNPs were developed using the high-intensity ...
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Background and objective: In the present paper, impacts of gold Nanoparticles (AUNPs) and Chick Embryo Extract Therapy (CEET) were investigated on the wound healing promoting properties that match the dynamic healing process of skin flap regeneration.Methods: AUNPs were developed using the high-intensity short-pulse beams mode of neodymium-doped: Yttrium, Aluminum, and Garnet photoablation method. Also, 45-µg extract from a 4-6 day chick embryo was used as a protein source for CEET. Dorsal Random Skin Flaps (RSFs) were created in forty-five Albino Wistar rats and they were accidentally assigned to 3 groups; G(C); group did not receive any treatment (control), G (AUNP); treated only with AUNPs, and G(CE+AUNP) the rats treated with AUNPs and CEET. During a random skin flap animal model, AuNPs and CE were used as solutions and convenient to deliver into the skin flap. The skin flap survival rate was evaluated in the following by a camcorder, and tissue studies were done at days 3, 6, and 9 after surgery by the hematoxylin & eosin and Masson trichrome staining.Result: Rats treated with the AUNPs and CEET exhibited remarkably increased flap survival than the others. Tissue studies showed that AUNPs and CEET caused induction of forming the new blood vessels and stimulated the inflammation-causing reaction in an initial phase of wound healing. Conclusion: Using AUNPs with CEET increases the potential for a dynamic recovery of skin flap regeneration.
Ali alirezaie alavijeh; Milad Rajabi; Farid Barati; Moosa Javdani; Iraj Karimi; Mohammad Barati; Mohsen Moradian
Abstract
Catgut was enriched with copper sulfate nanoparticles (CSNPs@Catgut), in order to develop a new composited suture with antibacterial and healing properties. Introducing copper sulfate nanoparticles to catgut was performed using a reverse micro-emulsion technique. It is an interesting method because of ...
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Catgut was enriched with copper sulfate nanoparticles (CSNPs@Catgut), in order to develop a new composited suture with antibacterial and healing properties. Introducing copper sulfate nanoparticles to catgut was performed using a reverse micro-emulsion technique. It is an interesting method because of easy handling and relatively low costs. In the revers micro-emulsion medium, nano-spherical structures containing the salt solution are created. The nano-spheres penetrate into catgut fibers and precipitate after drying to form the salt nanoparticles. The prepared CSNPs@Catgut was characterized using scanning electron microscopy, X-ray diffraction (XDR) technique, tensile strength, antibacterial activity, and cytotoxicity tests. XRD and SEM confirmed the CuSO4 nanoparticles formation and grafting on catgut surface. Antibacterial properties were illustrated by E. coli inhibition zone and CSNPs@Catgut showed a significant antibacterial activity compare with catgut. Results of cytotoxicity tests showed no difference between CSNPs@Catgut and catgut. The mechanical strength was improved and increased from 4.16 mm to 10.00 mm. Tissue reaction showed that necrosis and inflammatory cells were reduced from acute to subacute on day 14. Therefore, CSNPs@Catgut can be introduced as a reliable candidate for wound management.
