Kiranmayee Pamidimukkala; Prabhudas N.; Nandini H.S.; Kutty A.V.M.
Abstract
AbstractGreen based nanoparticles were synthesized to investigate the cytotoxic effects on human peripheral blood mononuclear cells (hPBMCs). Nanoparticles were made by mixing using aqueous Allamanda cathartica L. latex (3%) and magnesium oxide (1mM) at 370C on shaker for 24 hours. The developed nanoparticles ...
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AbstractGreen based nanoparticles were synthesized to investigate the cytotoxic effects on human peripheral blood mononuclear cells (hPBMCs). Nanoparticles were made by mixing using aqueous Allamanda cathartica L. latex (3%) and magnesium oxide (1mM) at 370C on shaker for 24 hours. The developed nanoparticles were characterized by Scanning Electron Microscopy, Transmission Electron Microscopy, Fourier Transmission Infra-Red, Zeta potential and X-ray diffraction. in vitro hPBMCs were tested in vitro with different concentrations of synthesized nanoparticles and found maximum concentration at that 100 µg/mL showed about 96% dead cells from trypan blue dye exclusion method. Though controlling the proliferation of cells, the nanoparticles did not exhibit any genotoxicity, indicating that the nanoparticles showed their effect on periphery of the cells, broke them that led to the death of cells. This is the first report of green route development of MgO nanoparticles using the latex of Allamanda cathartica L. and finding their efficacy on hPBMCs. Further analysis is required to confirm their use as therapeutics in leukemia.
Sharon Stephen; Toji Thomas
Abstract
Nanotechnology has immense number of applications in all contemporary fields of science. Silver nanoparticles form an important part of metal nanoparticles, with wider range of applications in diverse areas of science. Plant mediated green synthesized silver nanoparticles are usually preferred due to ...
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Nanotechnology has immense number of applications in all contemporary fields of science. Silver nanoparticles form an important part of metal nanoparticles, with wider range of applications in diverse areas of science. Plant mediated green synthesized silver nanoparticles are usually preferred due to its advantages over other metal nanoparticles viz. non toxicity, lesser energy consumption, cost effectiveness and lesser pollution. Plants belonging to Acanthaceae family are medicinally as well as pharmacologically relevant. They contain many important phytochemicals, which can reduce, stabilize and act as capping agents in the nanoparticle synthesis. Various mechanisms to synthesize silver nanoparticles are explored here to analyse its formations in the most efficient method. Different phytochemicals with various functional groups assisting the formation of nanoparticles during green synthesis are also summarized here. The synthesized silver nanoparticles show effective antimicrobial properties along with many other potential bioactivities. The mechanisms of antimicrobial properties of silver nanoparticles are also assessed. Various bioactivity studies connected with green synthesized AgNPs comprising of antimicrobial, antifungal, biocompatibility, anti-inflammatory, anticancer, antioxidant, larvicidal, effect on Seed germination and growth are briefly outlined in this article.
