@article { author = {Abbasi, Shahla and İlhan, Ahmet and jabbari, Hadi and Javidzade, Parisa and Safari, Maede and Abolhasani Zadeh, Firoozeh}, title = {Cytotoxicity evaluation of synthesized silver nanoparticles by a Green method against ovarian cancer cell lines}, journal = {Nanomedicine Research Journal}, volume = {7}, number = {2}, pages = {156-164}, year = {2022}, publisher = {Tehran University of Medical Sciences}, issn = {2476-3489}, eissn = {2476-7123}, doi = {10.22034/nmrj.2022.02.005}, abstract = {Objective(s): In recent years, the attention of many was focused on the features of the Green Synthesis process, such as its simplicity, cost-effectiveness, and environmental friendliness. This study investigates the feasibility of manufacturing silver nanoparticles from Malva sylvestris L aqueous extract and evaluates its anti-cancer effect on ovarian cancer cell lines OVCAR-3 and SK-OV-3. Methods: The aqueous extract of Malva sylvestris L proved to be capable of synthesizing silver nanoparticles (Ag-NPs) and the success of the procedure was affirmed through the results of characterizing analyzes such as UV-Vis spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). Additionally, we assessed the anti-cancer impacts of synthesized Ag-NPs on OVCAR-3 and SK-OV-3 cell lines by the means of MTT assay. The Bax, Bcl-2, caspase 3, and caspase 8 expression at mRNA levels were also estimated using real-time PCR.Results: The appearance of an absorption peak at 462 nm using a spectrophotometer signified the complete synthesis of silver nanoparticles. The electron microscope images showed that the nanoparticles were spherical and had an average size of about 74.4 nm. Ag-NPs at the concentration (10-200nM) inhibited the proliferation of OVCAR-3 and SK-OV-3 cell lines. Moreover, treatment resulted in down-regulating Bcl-2 while up-regulating Bax and caspase 3 and caspase 8 expression.Conclusions: In this study, synthesized Ag-NPs using Malva sylvestris L could induce a robust anti-cancer effect on ovarian cells in vitro by improving Bax/Bcl-2 ratio and triggering the apoptosis pathway.}, keywords = {Silver nanoparticle (Ag-NPs),Malva sylvestris L,Biosynthesis,Ovarian Cancer}, url = {https://www.nanomedicine-rj.com/article_253219.html}, eprint = {https://www.nanomedicine-rj.com/article_253219_7f17349a783d900d4539e24acc16ff9a.pdf} }