@article { author = {Mahmoudi, Hamed and Nouralishahi, Alireza and Mohammadzadehsaliani, Saman}, title = {Stem cell-derived nano-scale vesicles promotes the proliferation of retinal ganglion cells (RGCs) by activation PI3K/Akt and ERK pathway}, journal = {Nanomedicine Research Journal}, volume = {7}, number = {3}, pages = {288-293}, year = {2022}, publisher = {Tehran University of Medical Sciences}, issn = {2476-3489}, eissn = {2476-7123}, doi = {10.22034/nmrj.2022.03.008}, abstract = {Objective(s): To evaluate the effects of the mesenchymal stem cell-derived exosome on the proliferation of retinal ganglion cells (RGCs)-5 cell line.Methods: Exosomes were isolated from the human bone marrow (BM)-derived MSCs. Exosomes characteristic were verified by western blotting and transmission electron microscopy (TEM) image. The proliferation of the RGC-5 cell line was estimated in co-culture conditions with MSCs-exosome by MTT assay within 24-96 hours of exposure. The expression levels of the AKT, PI3K, and ERK pathways were measured in RGC-5 cells within 24-96 hours of exposure.Results: Based on the MTT assay results, exosomes at concentrations of 50-200 ng/ml enhanced the proliferation of the RGC-5 cell line in vitro. Also, this co-culture resulted in the up-regulating of the expression of AKT, PI3K, and ERK pathways in the RGC-5 cell line.Conclusions: Accordingly, we concluded that the exosome could stimulate the proliferation of the RGC-5 cell line by inducing PI3K/AKT and ERK axes.}, keywords = {MSCs,Exosome,RGCs,Proliferation}, url = {https://www.nanomedicine-rj.com/article_253904.html}, eprint = {https://www.nanomedicine-rj.com/article_253904_2d41ec6072c951f11945399fa1d826a2.pdf} }