%0 Journal Article %T Silver nanoparticles: biosynthesis and cytotoxic performance against breast cancer MCF-7 and MDA-MB-231 cell lines %J Nanomedicine Research Journal %I Tehran University of Medical Sciences %Z 2476-3489 %A Darvish, Soodabeh %A saeed Kahrizi, Mohammad %A Özbolat, Gülüzar %A Khaleghi, Fatemeh %A Mortezania, Zohreh %A Sakhaei, Delaram %D 2022 %\ 01/01/2022 %V 7 %N 1 %P 83-92 %! Silver nanoparticles: biosynthesis and cytotoxic performance against breast cancer MCF-7 and MDA-MB-231 cell lines %K Silver Nanoparticle %K Ducrosia Anethifolia %K Biosynthesis %K Breast cancer %K Viability %R 10.22034/nmrj.2022.01.008 %X Objective(s): A study was performed on the possibility of synthesizing silver nanoparticles (Ag-NPs) from Ducrosia Anethifolia Aqueous Extract for treating breast cancer MCF-7 and MDA-MB-231 cell lines.Methods: The application of Ducrosia Anethifolia Aqueous Extract caused a reduction in silver ions and facilitated the production of silver nanoparticles. Different analyzing procedures were exerted to evaluate the qualities of synthesized NPs by Ducrosia Anethifolia Aqueous Extract including UV-Vis spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). In the following, subsequent to assessing the viability of MCF-7 and MDA-MB-231 cell lines, their exposure to AgNPs was evaluated by the usage of MTT assay. Moreover, the Bax, Bad, Bcl-2, and c-FLIP expression levels of treated cells were examined by real-time PCR analysis.Results: The observance of an absorption peak at 460 nm through the obtained data from a spectrophotometer affirmed the production of silver NPs. The images of electron microscope displayed the spherical shape of NPs in an average size of around 9.41 nm. Moreover, this product caused a robust reduction in the viability of both MCF-7 and MDA-MB-231 cell lines that was mainly pointed out by the improved expressions of Bax and Bad gene, while minimizing the gene expressions of Bcl-2 and c-FLIP.Conclusions: This experiment approved the applicability of Ducrosia Anethifolia Aqueous Extract for performing the green synthesis of NPs. In addition, the synthesized NPs exhibited anti-tumor effects on the applied breast cancer cell lines through the up-regulation of pro-apoptotic proteins concomitant along with the down-regulation of anti-apoptotic proteins expression. %U https://www.nanomedicine-rj.com/article_251673_5c8fd729d8bb70284f981e48cbab8746.pdf