TY - JOUR ID - 253224 TI - Fabrication and characterization of chitosan/gelatin scaffold with bioactive glass reinforcement using PRP to regenerate bone tissue JO - Nanomedicine Research Journal JA - NMRJ LA - en SN - 2476-3489 AU - Ghomi, Farhad AU - Asefnejad, Azadeh AU - Daliri, Morteza AU - Godarzi, Vahabeddin AU - Hemati, Mehran AD - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran AD - School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran 3 Applied Biotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran. AD - Dental School, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2022 PY - 2022 VL - 7 IS - 2 SP - 205 EP - 213 KW - Bioglass KW - Chitosan-Gelatin membrane KW - Bone tissue KW - Freeze drying KW - Platelet-rich plasma DO - 10.22034/nmrj.2022.02.010 N2 - In this study, the purpose of this study was to fabricate a porous nanocomposite chitosan-gelatin along with different weight percentages of bioglass using the freeze-drying method. In this study, the Platelet-rich plasma (PRP) technique was used for the first time to strengthen bone grafting. Tensile strength, elastic modulus, and percentage of porosity were evaluated. Also, the samples were examined in simulated body fluid (SBF) and phosphate buffer saline (PBS) to study bone growth and bone dissolution rate.  Also, the porosity percentage was about 54% with 39% bone-like apatite formation for the third sample. The early absorption and late absorption rate of bone substitutes and the main volume of bone substitutes can be used for orthopedic approaches. The specimen were abalysized using X-ray diffraction (XRD) and scanning electron microscope (SEM) technique. Porous membranes were examined under SEM tools to estimate pore size and morphological behavior. The pore diameter can be controlled by adjusting the ethanol percentage in the range of 10-30 micrometers. The formation of needle bioglass crystals on the membrane surface after 7 days of immersion in biological solution was also evaluated using SEM images. In general, the third sample can be used to repair the bone tissue of the knee with the PRP technique. UR - https://www.nanomedicine-rj.com/article_253224.html L1 - https://www.nanomedicine-rj.com/article_253224_8d9b32ee1338b927759f638824e55da4.pdf ER -