TY - JOUR ID - 253899 TI - Sunlight-Assisted Fabrication of Silver Nanoparticles using Terminalia neotaliala Capuron Aqueous Bark Extract: Evaluation of In vitro Antioxidant and Anti-inflammatory Activities JO - Nanomedicine Research Journal JA - NMRJ LA - en SN - 2476-3489 AU - Vernekar, Nirupa Shivanand AU - Taranath, Tarikere Chandrashekarappa AD - Environmental Biology and Green Nanotechnology Laboratory, P. G. Department of Studies in Botany, Karnatak University, Dharwad-580003, Karnataka, India Y1 - 2022 PY - 2022 VL - 7 IS - 3 SP - 235 EP - 244 KW - Green synthesis KW - Terminalia neotaliala KW - Silver nanoparticles KW - HR-TEM KW - Surface Plasmon Resonance KW - Polyphenols DO - 10.22034/nmrj.2022.03.003 N2 - Objective(s): Sunlight-assisted rapid fabrication of silver nanoparticles (AgNPs) using aqueous bark extract of Terminalia neotaliala Capuron (TnB-AgNPs) and evaluation of in vitro antioxidant and anti-inflammatory activities. Methods: Aqueous bark extract of T. neotaliala was used as a reducing agent in AgNPs formation under direct sunlight. Techniques like UV-Vis Spectroscopy, FTIR, XRD, DLS, HR-TEM with SAED were employed for the characterization studies. Further, antioxidant activity was determined by DPPH radical scavenging assay and anti-inflammatory activity by BSA anti-denaturation assay.Results: The TnB-AgNPs formation was observed as a colour change from light-yellow to dark-brown with a λmax value of 414nm. FTIR spectra revealed different functional groups such as -OH, -CH, -C=O, -NH, -C-N groups associated with the presence of polyphenolic compounds in the bark extract. XRD confirmed the Face Centered Cubic crystalline nature with an average crystallite size of 26 nm. Z-average particle size was found to be 57nm with a zeta potential value of -40.9mV indicating excellent stability. HR-TEM studies depict spherical shape with average particle size of 34.81nm and the lattice planar spacing of 0.24nm. The TnB-AgNPs and bark extract showed % scavenging of 70.66±0.27% and 80.78±0.39 % (100 µg/mL) for antioxidant activity and % inhibition of 79.49±1.22% and 67.92±1.00% (500µg/mL) for anti-inflammatory activity at their highest concentration. Conclusion: The present study demonstrates a rapid approach in producing small, spherical, stable, crystalline AgNPs using T. neotaliala bark extract under the influence of direct sunlight. The synthesized TnB-AgNPs showed moderate antioxidant and excellent anti-inflammatory activity indicating its potentiality in biomedical applications.  UR - https://www.nanomedicine-rj.com/article_253899.html L1 - https://www.nanomedicine-rj.com/article_253899_31cc8a7351643e85dbde890b9097ca56.pdf ER -