TY - JOUR ID - 253902 TI - Mesenchymal stem/stromal (MSCs)-derived exosome inhibits retinoblastoma Y-79 cell line proliferation and induces their apoptosis JO - Nanomedicine Research Journal JA - NMRJ LA - en SN - 2476-3489 AU - Kheradjoo, Hadis AU - Nouralishahi, Alireza AU - Hoseinzade Firozabdi, Mohamad Saeid AU - Mohammadzadehsaliani, Saman AD - Department of Pathology, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran AD - Isfahan Eye Research Center, Isfahan University of Medical Sciences, Isfahan, Iran AD - Master of Science Graduated from Shahid Beheshti Medical University AD - Department of Ophthalmology, Rustaq Hospital, Rustaq, Oman Y1 - 2022 PY - 2022 VL - 7 IS - 3 SP - 264 EP - 269 KW - Exosome KW - Cancer KW - Retinoblastoma KW - Proliferation DO - 10.22034/nmrj.2022.03.006 N2 - Aims: Based on the literature, mesenchymal stem/stromal cell derived exosome has dual role in tumor therapy or progress. We evaluated the possible anti-tumor effect of the MSCs-exosome on retinoblastoma Y-79 cell cells in vitro.Methods: To verify the efficient isolation of exosome, the MSCs-exosome structure and the expression of CD9 and CD81 was examined using transmission electron microscopy (TEM) and western blotting, respectively. Y-79 cell proliferation and apoptosis were evaluated using MTT assay and flow cytometry, respectively. The expression levels of Bcl-2 and microRNA-143 were estimated by the Real-time PCR.Results: MSCs-exosome inhibited Y-79 cells proliferation as shown by MTT assay results. Also, MSCs-exosome induced apoptosis in Y-79 cells within 72 hours of treatment. Real-time PCR results also exhibited that exosome therapy resulted in a decrease in Bcl-2 expression while up-regulating microRNA-143 expression.Conclusion: MSCs-derived exosome could deter retinoblastoma Y-79 cells tumorigenicity, thereby providing novel modality to manage retinoblastoma patients. UR - https://www.nanomedicine-rj.com/article_253902.html L1 - https://www.nanomedicine-rj.com/article_253902_93b292f2eda94674077b17add5d786d1.pdf ER -