TY - JOUR ID - 253904 TI - Stem cell-derived nano-scale vesicles promotes the proliferation of retinal ganglion cells (RGCs) by activation PI3K/Akt and ERK pathway JO - Nanomedicine Research Journal JA - NMRJ LA - en SN - 2476-3489 AU - Mahmoudi, Hamed AU - Nouralishahi, Alireza AU - Mohammadzadehsaliani, Saman AD - Resident of Oral &Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Science, Tehran, Iran AD - Isfahan Eye Research Center, Isfahan University of Medica AD - Department of Ophthalmology, Rustaq Hospital, Rustaq, Oman Y1 - 2022 PY - 2022 VL - 7 IS - 3 SP - 288 EP - 293 KW - MSCs KW - Exosome KW - RGCs KW - Proliferation DO - 10.22034/nmrj.2022.03.008 N2 - Objective(s): To evaluate the effects of the mesenchymal stem cell-derived exosome on the proliferation of retinal ganglion cells (RGCs)-5 cell line.Methods: Exosomes were isolated from the human bone marrow (BM)-derived MSCs. Exosomes characteristic were verified by western blotting and transmission electron microscopy (TEM) image. The proliferation of the RGC-5 cell line was estimated in co-culture conditions with MSCs-exosome by MTT assay within 24-96 hours of exposure. The expression levels of the AKT, PI3K, and ERK pathways were measured in RGC-5 cells within 24-96 hours of exposure.Results: Based on the MTT assay results, exosomes at concentrations of 50-200 ng/ml enhanced the proliferation of the RGC-5 cell line in vitro. Also, this co-culture resulted in the up-regulating of the expression of AKT, PI3K, and ERK pathways in the RGC-5 cell line.Conclusions: Accordingly, we concluded that the exosome could stimulate the proliferation of the RGC-5 cell line by inducing PI3K/AKT and ERK axes. UR - https://www.nanomedicine-rj.com/article_253904.html L1 - https://www.nanomedicine-rj.com/article_253904_2d41ec6072c951f11945399fa1d826a2.pdf ER -