Synthesis of 1,5 and 2,5-disubstituted tetrazoles using NiO nanoparticles and their evaluation as antimicrobial agents

Document Type: Original Research Article


1 Department of Organic Chemistry, Faculty of Chemistry, University of Kashan, Iran

2 Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran


Treatments of tetrazolate salts prepared in situ from various aldehyde; malononitrile and sodium azide in the presence of NiO nanoparticles with benzyl bromide gave the corresponding 1,5- and 2,‌5-disubstituted tetrazoles. Reaction of tetrazolate salts with 2,4′-dibromoacetophenone in the presence of NiO nanoparticles provided the corresponding 2,5-disubstituted derivatives as an only isomer. The structures of the prepared tetrazoles were fully characterized by 1H and 13C NMR spectra, IR spectra, MS and elemental analysis. Use of simple and readily available starting materials, excellent yields, short reaction times, reusability of the catalyst, low amount of catalyst are some advantages of this protocol. Their antimicrobial activity has been tested in vitro against Gram negative bacteria; Gram positive bacteria and fungi. Four compounds have only moderate growth inhibitory effects against Gram positive bacteria. The antimicrobial screening suggests that compounds 5h; 6h; 5g and 6g have only moderate growth inhibitory effects against Gram positive bacteria. Among the newly synthesized compounds; good antimicrobial activity was observed for compound 6g against Staphylococcus epidermidis (MIC value 125 µg/ml).



The family of tetrazoles is known as a highly main moiety in organic; organometallic; medicinal chemistry [1-6], and in diverse materials science including propellants [7], and inflammables [8], 1,5- and 2,5-disubstituted tetrazoles are also significant as NAD(P)H oxidase inhibitors [9], potential TNF-α inhibitors [10], hepatitis C virus (HCV) serine protease NS3 inhibitors [11], selective cyclooxygenase-2 (COX-2) inhibitors [12], calcitonin gene-related peptide receptor antagonists, antimigraine [13], antiviral and antiproliferative activity [14, 15]. Several procedures have been developed in literature for the preparation of tetrazoles [16,17]. To prepare these compounds, the alkylation of tetrazole anions are utilized. However; owing to the ambient nature of the anions 1a1b, the metal salt products of 5-substituted tetrazoles undergo to alkylation with electrophiles in a vast range of solvents afforded mixture of 5-substituted 1N- and 2N-alkyl tetrazoles (Scheme 1) [18]. For instance; the reaction of 5-substituted tetrazoles with epoxy compounds [19] dialkyl sulfates [20], benzyl bromide [21], and alkyl halides [22], using base; or with diazomethane, [23] afford a mixture of 1,5 and 2,5-disubstituted tetrazoles and the ratio of the regioisomers are affected by the electronegativity and size of the 5-substituent. Even by obstructing the N(2)-position with tri-n-butyltin before alkylation; the 2,5-isomer was formed about a 10% yield [24].

Nelson et al prepared a series of complexes including of the 1,5- disubstituted tetrazoles individually by obstructing the 2-position with cobalt complexes [25]. Kondo et al obtained 2,­5-diarylsubstituted tetrazoles from phenyl sulfonyl hydrazones of aromatic aldehydes and arenediazonium salts [26]. Yamamoto and co-workers were prepared 2,5-disubstituted tetrazoles by palladium-catalyzed reaction of nitriles; trimethyl-silylazide, and allyl acetates [27].

Multi-component reactions (MCRs) are efficient and rapid methods for the preparation of single reactive intermediate or products from several starting materials [28,29]. Transition metal-catalyzed multi-component systems have recently increased substantial interest [30,31]. Lately, nickel-based nanoparticles and especially NiO nanoparticles have been utilizeed as effective heterogeneous catalysts for organic reactions [32-35]. Our interest on the preparation of heterocyclic compounds and the development of efficient methods for MCRs which were catalyzed by nanoparticles [36-38], led us to the generation of regioselected 2,5-disubstituted tetrazoles 4a-h by Knoevenagel condensation/1,3-dipolar cycloaddition reaction of aldehydes, sodium azide, malononitrile, and 2,4′-dibromoacetophenone using nano-NiO as a catalyst.

The catalyst were prepared according to the procedure presented by Zhang et al from the reaction of NaCl and Ni(OH)2 [39-41].

Recently we reported an efficient method for the synthesis of 2-(1H-tetrazol-5-yl) acrylonitrile derivatives (3) starting from aldehydes (1); malononitrile (2) and sodium azide continued by acidic hydrolysis [35]. The best result was achieved at 70°C with 6 mol % nanocrystalline in DMF.

We continued working to expand the groups of tetrazole heterocyclic compounds by beginning from this effective reagent. The first outcome of this project is the preparation of 1,5-disubstituted and 2,5-disubstituted tetrazoles from benzyl bromide and tetrazolate salts by an expeditious approach. Then we obtained the regioselected compounds of a series of new 2,5-disubstituted tetrazoles through treatment of tetrazolate salts with 2,4′-dibromoacetophenone (Scheme 2). The antimicrobial screening results against Gram negative bacteria; Gram positive bacteria and fungi are presented for compounds 4a4b4f5g5h6g and 6h.


General procedure for the preparation of disubstituted tetrazoles

Nano NiO (6 mol %) is added to a mixture of aromatic aldehyde (1.0 mmol); malononitrile (1.0 mmol) and NaN3 (1.0 mmol) in DMF (5 mL) and stirred at 70 °C. Then, the mixture was cooled to room temperature. Afterwards, benzyl bromide (1.0 mmol) or 2,4′-dibromoacetophenone (1.0 mmol) was added. The contents were further stirred at 70 °C until completion (monitoring by TLC). After completion; the nanocatalyst was separated off by centrifugation and rinsed with acetone (3 times). Water was added to precipitate. The precipitate was filtered and dried. Most of the compounds were obtained in pure form after easy trituration with ethyl acetate and hexane. Other compounds were purified by column chromatography (CHCl3/CH3OH 9.5:0.5). In a representative case (Entry 1; Table 1); the recovered catalyst was reused in three successive runs without any considerable reduce in the product yields.

Determination of Antimicrobial activity

Microbial strains

Products of 4a4b4f5g5h, 6g and 6h were appraised in vitro against for antibacterial activities against Pseudomonas aeruginosa (ATCC 27853); Escherichia Coli (ATCC 10536); Klebsiella pneumonia (ATCC 10031); Shigella dysenteriae (PTCC 1188); Proteus vulgaris (PTCC 1182) and Salmonella paratyphi-A serotype (ATCC 5702) as examples of Gram negative bacteria; Bacillus subtilis (ATCC 6633); Staphylococcus aureus (ATCC 29737) and Staphylococcus epidermidis (ATCC 12228) as examples of Gram positive bacteria. They were appraised in vitro for their antifungal activities against Candida albicans (ATCC 10231); Aspergillus niger (ATCC 16404) and Aspergillus brasiliensis (ATCC 16404) as examples of fungal strains.

Agar diffusion assay

Agar diffusion technique was utilized for determining preparatory antibacterial and antifungal activities [43]. Each of the test compounds was dissolved in DMSO as solvent to final concentration of 30 mg/ml and filtered by 0.45 µm Millipore filters for sterilization. One-hundred microliters of suspension including 10CFU/ml of bacteria; 106 CFU/ml of yeast and 104 spore/ml of fungi spread on the nutritious agar; sabouraud dextrose agar and potato dextrose agar medium; respectively. Uniform wells (6 diameters) were punched on the media plates and filled with 10 µl of the test compounds. Streptomycin (10µg/well) was utilized as positive control for bacteria and Nystatine (100 IU/well) for fungi. DMSO was applied as a negative control. The inseminated plates were incubated for at 37°C for 24 h bacterial strains and 48 h and 72 h at 30°C for yeast and mold isolated; respectively. The results were noted for each tested compound as average diameter of inhibition zones of bacterial and fungal around the wells in mm and each test was repeated twice.

Micro-well dilution assay

Bacterial strains sensitive to the compounds in agar diffusion assay were investigated for their minimum inhibitory concentration (MIC) values using micro-well dilution assay procedure [44]. The inocula of microbial strains were provided from 12 h broth cultures and suspensions were modified to 0.5 McFarland standard turbidity. The compounds were dissolved in 10% DMSO as solvent and diluted to the highest concentration (2000 µg/ml) to be tested and then serial twofold dilutions were prepared in a concentration range from 31.25 to 2000 µg/ml in 10 ml sterile tubes including brain heart infusion (BHI) broth. The 96-well plates were provided by dispensing 95 µl of the cultures media and 5 µl of the inoculums into each well. A 100 µl aliquot from the stock solutions of the compounds was made at the concentration of 2000 µg/ml was added into the first well. Then 100 µl from their serial dilutions was transferred into six successive wells. The last well comprising 195 µl of the cultures media without the test materials and 5 µl of the inoculums on each strip was applied as the negative control. Streptomycin was utilized as standard drug for positive control in conditions similar to tests materials. Turbidity indicated growth of microorganism and the MIC were determined as the lowest concentrations of the compounds that prevented visible growth.



The morphology of nano-NiO was determined by Scanning Electronic Microscopy (SEM). The results from SEM images clearly demonstrate that the average size of nano-NiO is about nanometers (Fig. 1).

Fig. 1

It has been reported formerly that the reaction of tetrazolate salts with halogenoalkanes gave the 1,5-disubstituted and 2,5-disubstituted tetrazoles as mixtures [21,22]. Treatment of tetrazolate salts prepared in situ with benzyl bromide offered the corresponding mixtures of 1,5-disubstituted 5 (minor isomers) and 2,5- disubstituted 6 (major isomers) derivatives (Table 1; entry 9, 10). TLC of this solution indicated them to be a mixture of two products; which was separated by silica gel column chromatography using CHCl3/CH3OH as eluent. In their mass spectra; both of these compounds; the low (5g, 5h) and the high (6g, 6h) moving; showed the same molecular ion peak illustrated them to be the positional isomers. In the 13C NMR spectra the carbon CHattached to tetrazole are very obvious, appearingat ca. 51.6 ppm in isomers 5h and at ca. 56.8 ppm in isomers 6h, also vinylic carbon attached to nitrile group (88.87 ppm for isomer 5h and 93.35 ppm for isomer 6h. In 1H NMR spectra of (E)-2- (2-benzyl- 2H-tetrazol-5-yl)-3-(4-hydroxyphenyl) acrylonitrile (6h) the appearance of resonance signal for CHattached to tetrazole ring at 5.96 is relatively at higher field than the resonance signals at 5.85 for CH2 attached to tetrazole ring in (E)-2-(1-benzyl-1 H-tetrazol-5-yl)-3- (4- hydroxy phenyl) acrylonitrile (5h) further supporting their assigned structures. The ratio of isolated yields of the above two isomers (ratio of 6g/5g is 8.6 and ratio of 6h/5h is10.8) determined by 1H NMR. These results suggested that the 6g;h isomers are the predominant ones (Table 1). Steric factors also have a vital role in the ratio for formation of isomers [40-42].

Treatment of tetrazolate salts with 2,4′-dibromoacetophenone gave the corresponding 2,­5-disubstituted derivative as an only isomer. TLC of the reaction mixture showed the product to be one isomer. The best evidences for the formation of 2,5-disubstituted derivative are its less steric hindrance which is explained in suggested mechanism and the appearance of a deshielded singlet for CH2attached to tetrazole ring (6-7 ppm) in the 1H NMR spectrums of 4a-4h.

Antimicrobial activity

The results of antimicrobial activity of compounds are presented in Table 2 and 3. Our results demonstrated that the synthetic compounds 4a, 4b4f, 5g, 5h, 6g and 6h have no antimicrobial effect against Gram negative bacteria and fungi. The compounds 5h; 6h; 5g and 6g have only moderate growth inhibitory effects against Gram positive bacteria (Bacillus subtilisStaphylococcus aureus andStaphylococcus epidermidis) (Table 2).

As displayed in Table 3; the results of the MIC values of the elected compounds in all cases were more than 500 µg/ml against Bacillus subtilis and Staphylococcus aureus. Compound 5h and 6h showed MIC values 250 µg/ml against Staphylococcus epidermidis and good antimicrobial activity was apperceived for compound 6g against Staphylococcus epidermidis (MIC value 125 µg/ml).


In conclusion, we have improved an efficient procedure for the preparation of disubstituted tetrazoles in the present of NiO nanoparticles. Reaction of tetrazolate salts and 2,4′-dibromoacetophenone in the presence of NiO NPs provided the regioselected products. This procedure offers several advantages; containing facile; excellent yields in short time; ease of experimental method; and environmentally friendly. The antimicrobial screening suggests that compounds 5h6h5g and 6g have only medium growth inhibitory effects against Gram positive bacteria. Among the newly synthesized compounds; good antimicrobial activity was observed for compound 6g against Staphylococcus epidermidis (MIC value 125 µg/ml).

Supporting Information

Experimental method and product characteri-zation data: IR, 1H NMR, 13C NMR and elemental analyses of the selected compounds are presented in Supporting Information.


The authors are grateful to university of Kashan for supporting this work.


The authors declare that there are no conflicts of interest regarding the publication of this manuscript.



1. Singh H, Singh Chawla A, Kapoor VK, Paul D, Malhotra RK. 4 Medicinal Chemistry of Tetrazoles. Progress in Medicinal Chemistry: Elsevier; 1980. p. 151-83.

2. Katritzky A, Cai C, Meher N. Efficient Synthesis of 1,5-Disubstituted Tetrazoles. Synthesis. 2007;2007(8):1204-8.

3. Jursic BS, Leblanc BW. Preparation of tetrazoles from organic nitriles and sodium azide in micellar media. Journal of Heterocyclic Chemistry. 1998;35(2):405-8.

4. Yella R, Khatun N, Rout SK, Patel BK. Tandem regioselective synthesis of tetrazoles and related heterocycles using iodine. Organic & Biomolecular Chemistry. 2011;9(9):3235.

5. Herr RJ. 5-Substituted-1H-tetrazoles as carboxylic acid isosteres: medicinal chemistry and synthetic methods. Bioorganic & Medicinal Chemistry. 2002;10(11):3379-93.

6. Romagnoli R, Baraldi PG, Salvador MK, Preti D, Aghazadeh Tabrizi M, Brancale A, et al. Synthesis and Evaluation of 1,5-Disubstituted Tetrazoles as Rigid Analogues of Combretastatin A-4 with Potent Antiproliferative and Antitumor Activity. Journal of Medicinal Chemistry. 2011;55(1):475-88.

7. Butler RN. Recent Advances in Tetrazole Chemistry. Advances in Heterocyclic Chemistry Volume 21: Elsevier; 1977. p. 323-435.

8. Chavez DE, Hiskey MA, Gilardi RD. 3,3′-Azobis(6-amino-1,2,4,5-tetrazine): A Novel High-Nitrogen Energetic Material. Angewandte Chemie International Edition. 2000;39(10):1791-3.

9. Shi L, Wang R, Yang H, Jiang Y, Fu H. Efficient copper-catalyzed domino synthesis of tetrazoloisoquinolines. RSC Advances. 2013;3(18):6278.

10. Srihari P, Dutta P, Rao RS, Yadav JS, Chandrasekhar S, Thombare P, et al. Solvent free synthesis of 1,5-disubstituted tetrazoles derived from Baylis Hillman acetates as potential TNF-α inhibitors. Bioorganic & Medicinal Chemistry Letters. 2009;19(19):5569-72.

11. Shaabani A, Mofakham H, Mousavifaraz S. A Two-Step Synthesis of 1H-Tetrazolyl-1H-1,4-benzonitriles and 1H-Tetrazolyl-benzo[b][1,4]diazepines. Synlett. 2012;23(05):731-6.

12. Al-Hourani BJ, Sharma SK, Mane JY, Tuszynski J, Baracos V, Kniess T, et al. Synthesis and evaluation of 1,5-diaryl-substituted tetrazoles as novel selective cyclooxygenase-2 (COX-2) inhibitors. Bioorganic & Medicinal Chemistry Letters. 2011;21(6):1823-6.

13. Challa NR, Mamidisetty B, Ghanta MR, Padi PR. Synthesis and pharmacological evaluation of 5-[2′-(1H-tetrazol-5-yl)-biphenyl-4-ylmethyl]-4,5,6,7-tetrahydro-thieno[3,2-c]pyridine derivatives as platelet aggregation inhibitors. Journal of Saudi Chemical Society. 2014;18(5):513-9.

14. Chang C-S, Lin Y-T, Shih S-R, Lee C-C, Lee Y-C, Tai C-L, et al. Design, Synthesis, and Antipicornavirus Activity of 1-[5-(4-Arylphenoxy)alkyl]-3-pyridin-4-ylimidazolidin-2-one Derivatives. Journal of Medicinal Chemistry. 2005;48(10):3522-35.

15. Kaplancıklı ZA, Yurttaş L, Özdemir A, Turan-Zitouni G, Çiftçi GA, Yıldırım ŞU, et al. Synthesis and antiproliferative activity of new 1,5-disubstituted tetrazoles bearing hydrazone moiety. Medicinal Chemistry Research. 2013;23(2):1067-75.

16. Najafi P, Modarresi-Alam A.R, One-Step Synthesis of Sterically Hindered 1, 5-Disubstituted Tetrazoles from Bulky Secondary N-Benzoyl Amides Using Triazidochlorosilane (TACS), Res. J. Chem. Env. Sci. 2013;1:28-33.


18. Jonassen HB, Nelson JH, Schmitt DL, Henry RA, Moore DW. Platinum- and palladium-tetrazole complexes. Inorganic Chemistry. 1970;9(12):2678-81.

19. Upadhayaya RS, Jain S, Sinha N, Kishore N, Chandra R, Arora SK. Synthesis of Novel Substituted Tetrazoles Having Antifungal Activity. ChemInform. 2004;35(45).

20. Henry RA, Finnegan WG. Mono-alkylation of Sodium 5-Aminotetrazole in Aqueous Medium. Journal of the American Chemical Society. 1954;76(3):923-6.

21. Aridoss G, Laali KK. Building Heterocyclic Systems with RC(OR)2+ Carbocations in Recyclable Brønsted Acidic Ionic Liquids: Facile Synthesis of 1-Substituted 1H-1,2,3,4-Tetrazoles, Benzazoles and Other Ring Systems with CH(OEt)3 and EtC(OEt)3 in [EtNH3][NO3] and [PMIM(SO3H)][O. European Journal of Organic Chemistry. 2011;2011(15):2827-35.

22. Romert M, Herbst RM, The structure of alkylated 1-alkyl-5-aminotetrazoles, J. Org. Chem. 1954;19:439-440.

23. Markgraf JH, Bachmann WT, Hollis DP. Proton Magnetic Resonance Spectra of Certain Methyltetrazoles. The Journal of Organic Chemistry. 1965;30(10):3472-4.

24. Gyoung YS, Shim J-G, Yamamoto Y. Regiospecific synthesis of 2-allylated-5-substituted tetrazoles via palladium-catalyzed reaction of nitriles, trimethylsilyl azide, and allyl acetates. Tetrahedron Letters. 2000;41(21):4193-6.

25. Takach NE, Holt EM, Alcock NW, Henry RA, Nelson JH. Regiospecific coordination of ambidentate tetrazoles to cobalt oximes. Journal of the American Chemical Society. 1980;102(9):2968-79.


27. Herr RJ. 5-Substituted-1H-tetrazoles as carboxylic acid isosteres: medicinal chemistry and synthetic methods. Bioorganic & Medicinal Chemistry. 2002;10(11):3379-93.

28. Bazgir A, Ghahremanzadeh R, Shakibaei G. An Efficient One-Pot Synthesis of 1H-Pyrazolo[1,2-b]phthalazine-5,10-dione Derivatives. Synlett. 2008;2008(8):1129-32.

29. Jia X, Chen X, Huo C, Peng F, Qing C, Wang X. Synthsis of 4‐Piperidones via Multicomponent Double Mannich Reaction Catalyzed by I 2. Chinese Journal of Chemistry. 2012;30(7):1504-10.

30. Müller T, Schramm O, Oeser T, Kaiser M, Brun R. Rapid One-Pot Synthesis of Antiparasitic Quinolines Based upon the Microwave-Assisted Coupling-Isomerization Reaction (MACIR). Synlett. 2008;2008(3):359-62.

31. Gong H-Y, Rambo BM, Nelson CA, Cho W, Lynch VM, Zhu X, et al. Multi component self-assembly: supramolecular organic frameworks containing metal–rotaxane subunits (RSOFs). Dalton Trans. 2012;41(4):1134-7.

32. Polshettiwar V, Baruwati B, Varma RS. Nanoparticle-supported and magnetically recoverable nickel catalyst: a robust and economic hydrogenation and transfer hydrogenation protocol. Green Chem. 2009;11(1):127-31.

33. Kalbasi RJ, Mosaddegh N. Suzuki-Miyaura Cross-coupling Reaction Catalyzed by Nickel Nanoparticles Supported on Poly(N-vinyl-2-pyrrolidone)/TiO2-ZrO2Composite. Bulletin of the Korean Chemical Society. 2011;32(8):2584-92.

34. Rahdar A, Aliahmad M, Azizi Y, Keikhah N, Moudi M, Keshavarzi F, CuO-NiO Nano composites: Synthesis, Characterization, and Cytotoxicity evaluation, Nanomed Res J 2017;2:78-86.

35. Safaei-Ghomi J, Paymard-Samani S. Facile and Rapid Synthesis of 5-Substituted 1H-Tetrazoles VIA a Multicomponent Domino Reaction Using Nickel(II) Oxide Nanoparticles as Catalyst. Chemistry of Heterocyclic Compounds. 2015;50(11):1567-74.

36. Safaei-Ghomi J, Shahbazi-Alavi H, Heidari-Baghbahadorani E. ZnFe2O4 Nanoparticles as a Robust and Reusable Magnetically Catalyst in the four Component Synthesis of [(5-hydroxy-3-methyl-1H-pyrazol-4yl) (phenyl) Methyl]propAnedinitriles and Substituted 6-Amino-Pyrano[2,3-c]Pyrazoles. Journal of Chemical Research. 2015;39(7):410-3.

37. Safaei-Ghomi J, Heidari-Baghbahadorani E, Shahbazi-Alavi H. ChemInform Abstract: SnO Nanoparticles: A Robust and Reusable Heterogeneous Catalyst for the Synthesis of 3,4,5-Substituted Furan-2(5H)-ones. ChemInform. 2015;46(23):no-no.

38. Safaei-Ghomi J, Kalhor S, Shahbazi-Alavi H, Asgari-Kheirabadi M. Three-component synthesis of cyclic $beta $-aminoesters using CeO$_{2}$ nanoparticles as an efficient and reusable catalyst. TURKISH JOURNAL OF CHEMISTRY. 2015;39:843-9.

39. Zheng Y-Z, Zhang M-L. Preparation and electrochemical properties of nickel oxide by molten-salt synthesis. Materials Letters. 2007;61(18):3967-9.

40. Henry RA, Finnegan WG. Mono-alkylation of Sodium 5-Aminotetrazole in Aqueous Medium. Journal of the American Chemical Society. 1954;76(3):923-6.

41. Butler RN, Scott FL. Methylation Studies on Arylidene-5-tetrazolylhydrazones1a,b. The Journal of Organic Chemistry. 1966;31(10):3182-7.

42. Kitazaki T, Tamura N, Tasaka A, Matsushita Y, Hayashi R, Okonogi K, et al. Optically Active Antifungal Azoles. VI. Synthesis and Antifungal Activity of N-((1R,2R)-2-(2,4-Difluorophenyl)-2-hydroxy-1-methyl-3-(1H-1,2,4-triazol-1-yl)propyl)-N’-(4-substituted phenyl)-3(2H,4H)-1,2,4-triazolones and 5(1H,4H)-tetrazolones. CHEMICAL & PHARMACEUTICAL BULLETIN. 1996;44(2):314-27.

43. Khalifa NM, Al-Omar MA, Amr AE. Synthesis and characterization of some novel 7-(aryl)-3-phenyl-6-(1H-tetrazol-5-yl)-5H-thiazolo[3,2-a]pyrimidin-5-one derivatives. Russian Journal of General Chemistry. 2017;87(7):1618-20.

44. Güllüce M, Sökmen M, Şahin F, Sökmen A, Adigüzel A, Özer H. Biological activities of the essential oil and methanolic extract ofMicromeria fruticosa(L) Druce sspserpyllifolia(Bieb) PH Davis plants from the eastern Anatolia region of Turkey. Journal of the Science of Food and Agriculture. 2004;84(7):735-41.