@article { author = {Thawini, Haneen and AL-SHAWI, ALI}, title = {A polysaccharide of Ziziphus spina-Christi L., and it's Silver nanoparticles induced reactive oxygen species and late apoptosis of Liver cancer cells}, journal = {Nanomedicine Research Journal}, volume = {6}, number = {3}, pages = {237-247}, year = {2021}, publisher = {Tehran University of Medical Sciences}, issn = {2476-3489}, eissn = {2476-7123}, doi = {10.22034/nmrj.2021.03.004}, abstract = {Objective:Ziziphus spina-Christi is a Ziziphus genus, and its extracts have been tested for antimicrobial, antifungal, and antitumor properties. A polysaccharide and its silver nanoparticle from the Ziziphus spina-Christi plant have not yet been tested for their ability to fight liver cancer. Methods:Using a microwave-assisted extraction method, we extracted polysaccharide from the leaves and synthesized silver nanoparticles. The polysaccharide amount, availability, and nanoparticle properties were investigated using the phenol-sulfuric acid process, FT-IR, SEM, TEM, and XRD. The anticancer activity of crude polysaccharide and its polysaccharide coated AgNPs were investigated using the HepG2 cancer cells,MTT assay, cell morphology, cell cycle, reactive oxygen species (ROS), and apoptosis. Results:The existence of predominantly spherical shapes and various sizes (24.10, 29.77, 31.63, 47.7, 56, and 71.5) nm polysaccharide coated AgNPs was confirmed by SEM and TEM analysis. Using the Debye-Scherrer formula, XRD analysis revealed six peaks corresponding to the crystalline size (D value) of AgNPs. Polysaccharide-coated AgNPs outperformed raw polysaccharide against HepG2 cancer cells. Polysaccharide had an IC50 of 1.5 mg/ml in the MTT assay, while polysaccharide coated AgNPs had an IC50 of 0.705 mg/ml. Raw polysaccharide arrested cells in the S process, while polysaccharide coated AgNPs arrested cells in the G1 phase, according to flow cytometry study. Furthermore, raw polysaccharide late apoptosis was lower than late apoptosis of nanoparticles induced by reactive oxygen species. Conclusion:The findings indicated that polysaccharide coated AgNPs derived from Ziziphus spina-Christi polysaccharide could be developed as an anticancer agent against liver cancer and that further research for potential applications is warranted}, keywords = {biopolymers,Zizphus spina-christi species,Polysaccharides,Polysaccharide coated AgNPs,Flowcytometric analysis}, url = {https://www.nanomedicine-rj.com/article_247464.html}, eprint = {https://www.nanomedicine-rj.com/article_247464_aee3cb66f8daacacf461167267f14956.pdf} }