@article { author = {Saeed Kahrizi, Mohammad and Maghsoodi, Sairan and Ghayoumi, Faraz and Etemadi, Hasti and Aminzadeh, Soheila and Safa, Kimia and Alesaeidi, Samira and Saad Ahmed, Omar}, title = {Mesenchymal stem/stroma cell (MSC)-derived nanoscale exosomes inhibit T cell proliferation by negative regulation of PI3K/Akt and MAPK/ERK pathway}, journal = {Nanomedicine Research Journal}, volume = {7}, number = {2}, pages = {189-194}, year = {2022}, publisher = {Tehran University of Medical Sciences}, issn = {2476-3489}, eissn = {2476-7123}, doi = {10.22034/nmrj.2022.02.008}, abstract = {Objective(s): Exosomes, as membrane-enclosed nanovesicles (30–150 nm), transports active biomolecules between various cells. As natural nanoparticles (NP), they serve a key role in the diagnosis, treatment, as well as prevention of diseases. Recently it has been verified that MSC-derived exosomes are capable of adjusting immune cells' biological processes. We investigated the effects of the MSCs-derived exosome on T cell proliferation.Methods: xosomes isolated from the supernatant of bone marrow-MSC. The ultrastructure and shape of exosomes were evaluated via transmission electron microscopy (TEM), and CD9, CD63, and CD81 were detected by Western blotting. Then, we examined the effects of MSC-derived exosome on the proliferation of the T cells by MTT assay. Moreover, the expression levels of the PI3K, Akt, MAPK, and ERK were estimated at mRNA levels by Real-Time PCR. Results: We showed that MSCs-derived exosome inhibited T cell proliferation based on the MTT assay results. Real-time PCR analysis also exhibited that exosome co-culture resulted in down-regulation of PI3K, Akt, MAPK, and ERK expression levels.Conclusions: MSCs-derived exosome inhibits T cell proliferation by negative regulation of the survival- and proliferation-involved PI3K/Akt and MAPK/ERK pathway in vitro.}, keywords = {Stem Cell,nanovesicles,T cell,Proliferation}, url = {https://www.nanomedicine-rj.com/article_253222.html}, eprint = {https://www.nanomedicine-rj.com/article_253222_5351a61812b975cd71607bb159a6827b.pdf} }