Tehran University of Medical Sciences Nanomedicine Research Journal 2476-3489 6 3 2021 09 01 In-vitro Antioxidant and Cytotoxicity (sk-mel-3 cell) Activity of Green Synthesised Copper Nanoparticle using P. pellucida Plant Aqueous Extract 279 286 247469 10.22034/nmrj.2021.03.008 EN Shilapa V P Department of Pharmaceutics, Karuna College of Pharmacy Iringuttoor, Palakkad, Kerala, India Wilson Vilgeena Department of Pharmaceutics, Sanjo College of Pharmaceutical Studies, Vellapara, Palakkad, Kerala, India Bhagyasree S Department of Pharmaceutics, KVM College of Pharmacy, cherthala, Kerala, India, 688527 E Alby Babu Department of Pharmaceutics, Sanjo College of Pharmaceutical Studies, Vellapara, Palakkad, Kerala, India Davis Nidhina Department of Pharmaceutics, Sanjo College of Pharmaceutical Studies, Vellapara, Palakkad, Kerala, India Kurup Meena Vinayaka Mission College of Pharmacy, Salem, Tamil Nadu Muddukrishnaiah K Department of Phaemaceutical Technology, Anna University, Chennai 0000-0002-5411-368X Journal Article 2021 05 15 Green synthesis of metallic nanoparticles using medicinal plant extract treated to environmentally friendly, stable, cost-effective. Present work to study In-vitro antioxidant and cytotoxicity of green synthesised CuNPs using P. Pellucida plant extract. Copper nanoparticles were synthesised biogenically using P. Pellucida plant aqueous extract. CuNPs Characterised by using FE-SEM, U.V., FT-IR, EDAX. Biogenically synthesised copper nanoparticles conducted in-vitro Antioxidant (DPPH) and cytotoxicity (SK-MEL-3 cell). The results CuNPs were characterised by using U.V. spectroscopy absorbance 575 nm. Scanning electronic microscope showing the distribution and shape (5-20 nm) of the nanoparticles. X-ray spectrum showed different peaks for CuNPs detected at 2θ values 35.45°, 44.32°, and 65.25°. EDAX elemental spectroscopy conformed to the copper metal inside the nanoparticle (69.7%). The synthesised CuNPs showed good free radical scavenger activity than the ascorbic acid, and it also showed significant cytotoxicity (16μg/mL) against human skin cell lines (SK-MEL-3 cell). The current study conclusion recommended that green synthesised (P. Pellucida) CuNPs be used for therapeutic application. https://www.nanomedicine-rj.com/article_247469_2625b995c008a2f5f62cb40be9bf60cd.pdf