Tehran University of Medical Sciences Nanomedicine Research Journal 2476-3489 7 1 2022 01 01 In-vitro investigation of curcumin coated gold nanoparticles effect on human colorectal adenocarcinoma cell line 66 72 251671 10.22034/nmrj.2022.01.006 EN Abolfazl Akbari Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran 0000-0002-2151-4639 Zahra Shokati Eshkiki Alimentary Tract Research Center, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Sara Mayahi Radiation Biology Research center, Iran University of Medical Sciences, Tehran, Iran Seyed Mohammad Amini Radiation Biology Research center, Iran University of Medical Sciences, Tehran, Iran 0000-0001-6447-9872 Journal Article 2022 01 03 Curcumin is a herbal supplement that has been mentioned for many biomedical applications. Several pieces of research demonstrated that curcumin could improve cancer chemotherapy, lagging the metastasis progress, and prevent healthy cells from radiation therapy damage. Through a simple and green synthesis procedure, stable gold nanoparticles were synthesized by natural phytomedicine curcumin. The curcumin-coated gold nanoparticles (Cur@AuNPs) are red and represent the distinct gold nanoparticles' plasmonic peak. The average diameter of the synthesized nanoparticles is 21.7. However, the hydrodynamic diameter was 45.1 nm. The cytotoxicity of Cur@AuNPs has been investigated through an MTT assay. 24-hour treatment of Cur@AuNPs could eradicate more than 30% of HT29 cancerous cells. The real-time polymerase chain reaction (PCR) technique was used to study the molecular change in apoptotic protein changes. The nanoparticle treatment increases the level of the pro-apoptotic protein (Bax, P53, and P21) and decreases the anti-apoptotic protein level (Bcl-2) in the treated cell population. https://www.nanomedicine-rj.com/article_251671_6007d2817a1b33b71c4031a66392c4d5.pdf