Tehran University of Medical Sciences Nanomedicine Research Journal 2476-3489 11 1 2026 03 01 Hybrid Aptamer/AgNps Modified Electrode for Target Detection of Methamphetamine 73 83 737696 10.22034/nmrj.2026.01.008 EN Rana Eftekhar Nahli Department of Chemistry, Ah. C., Islamic Azad University, Ahar, Iran Saeideh Ebrahimiasl Department of Toxicology, Ah. C., Islamic Azad University, Ahar, Iran 0000-0002-6465-7605 Mahdi Zamanfar Department of Medical Science, Kazan University, Kazan, Russia Journal Article 2025 10 11 A novel method for the determination of methamphetamine was investigated based on a glassy carbon electrode (GCE) treated by aptamer/ silver nanoparticles (AgNPs) hybrid layer. Methamphetamine specific aptamer and AgNPs was immobolized on the electrode surface. For this purpose, the functionalized sequence of the methamphetamine binding aptamer (MBA) was immobilized by the amine group and the covalent bond on the GCE. The binding of methamphetamine to its specific aptamer sequence results in a change in the aptamer spatial structure desired and, as a result, changes the electron transfer kinetic and oxidation peak position. Cyclic voltammetric (CV), differential pulse voltammetric (DPV) and electrochemical impedance spectroscopy (EIS) methods were used to study occurred changes in electrochemical behavior. Presence of silver nanoparticles in the assembly of the electrode improved response time and sensitivity. A linear response to methamphetamine in the 10 nM to 630 nM concentration range was obtained with the detection limit of 1.03 nM (at an S/N ratio of 3) and quantitation limit of 3.126 nM. The proposed sensor represented a promising platform for the selective and sensitive determination of methamphetamine. https://www.nanomedicine-rj.com/article_737696_9fe3c2cd68054dc8f709b62ade4f2e79.pdf