Nanomedicine Research Journal

Nanomedicine Research Journal

Synthesis of C-dot by hydrothermal method and evaluation of its anti-bacterial effect against antibiotic resistant S. areus and K. pneumonea

Document Type : Original Research Article

Authors
Hamid Pajavand 1
Ashraf Mohabati Mobarez 1
Maryam Nikkhah 2
Ramin Abiri 3
Mohammad Raza Delnavazi 4
1 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2 Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University Tehran, Iran
3 Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
4 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
10.22034/nmrj.2023.02.006
Abstract
Objective(s): Carbon dots (C-dots) are an emerging class of engineered nanomaterials with broad applications in medicine, bio-imaging, sensing, electronic devices, and catalysis. The study aimed to synthesize carbon nanoparticles with antibacterial therapeutic properties against clindamycin-resistant Staphylococcus aureus and ciprofloxacin-resistant Klebsiella pneumonia strains.
Methods: The C-dots were prepared by a hydrothermal method. Then the synthesized carbon dot were characterized by UV-visible spectroscopy, dynamic light scattering, Fourier transform infrared spectroscopy and transmission electron microscopy. The minimum inhibitory concentration of C-dots was evaluated by the micro-broth dilution method. Antibiotic susceptibility testing was performed using the disk diffusion method.
Results: The C-dots significantly reduced S. aureus and K. pneumoniae strains growth when compared to untreated bacteria (control; P < 0.05). Therefore, the minimum inhibitory concentration (MIC) of C-dots for clindamycin-resistant S. areus and ciprofloxacin-resistant K. pneumoniae strains were 500 and 250 µg/ml, respectively. 
The survival percentage of S. areus and K. pneumoniae decreased to 48.05% and 11.6% respectively after treatment with 250 μg/ml C-dots. However, the viability of bacteria decreased to 3.8% and 2.5% at the concentration of 500 μg/ml. 
Conclusions: The results show that by producing antibacterial drugs at the nanoscale, C-dots are a promising new approach to improve the effectiveness of treating infections caused by antibiotic-resistant bacterial strains.
Keywords
Carbon dots
Klebsiella pneumonia
Staphylococcus aureus
Antimicrobial resistance
Subjects
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Nanomedicine Research Journal
Volume 8, Issue 2
Spring 2023
Pages 167-176