Nanomedicine Research Journal

Nanomedicine Research Journal

Mesenchymal stem cell derived exosome promotes the viability of ameloblast-like cells

Document Type : Original Research Article

Authors
Bardia Mortezagholi 1
Esmaeel Dadgar 2
Sadaf Saeidi 3
Leila Rajaei Behbahani 4
Alireza Firouzi 5
Omolbanin Rezayee Mehr 6
Emad Movahed 7
1 Dental Research Center, Faculty of Dentistry, Islamic Azad University of Medical Sciences, Tehran, Iran.
2 Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran
3 School of Dentistry, Centro Escolar University, Manila, Philippines
4 Dentist and Oral medicine specialist , Ahvaz, Iran.
5 Faculty of Dentistry, Master (Periodontology) of Medical Sciences, Kuala Lumpur University, Malaysia.
6 Health Center of Zabol University of Medical Sciences. Zabol .Iran
7 Dental Materials Research Center, Dental School, Islamic Azad University of Medical Sciences, Tehran, Iran.
10.22034/nmrj.2023.02.008
Abstract
Objective(s): To assess the effects of the exosome produced by mesenchymal stem cells (MSCs) on ameloblast-like cells' capacity to proliferate.
Methods: The exosomes were isolated from the human BM-MSCs and characterized by TEM images and western blotting. Then ALC cells were exposed with the increasing concentration of the exosome within 12, 24, 48 and 72 hours of treatment. Then, the cell viability was assessed by MTT assay. Also, the expression levels of the cyclin A, cyclin B, PI3K/AKT and FOXO3 were measured by real-time PCR upon cell exposure with 40 ng/ml exosome within 24-72 hours of treatment.
Results: BM-MSCs-exosome could to promote the viability of ALC cells, in particular, at higher concentrations. Also, therapy resulted in an increased level of cyclin A/B, PI3K, AKT and FOXO3 in treated cell, more evidently within 72 hours of treatment.
Conclusions: We showed that MSCs-derived exosome as natural nanoparticles could improve the viability of ameloblast-like cells by promoting cell cycle arrest and activating P3K/AKT pathway.
Keywords
Mesenchymal stem cell
Viability
PI3K/AKT
Cyclin A and Cyclin B
Subjects
  1. Relaño-Ginés A, Lehmann S, Deville de Périère D, Hirtz C. Dental stem cells as a promising source for cell therapies in neurological diseases. Critical Reviews in Clinical Laboratory Sciences. 2019;56(3):170-81. https://doi.org/10.1080/10408363.2019.1571478
  2. Yamada Y, Nakamura-Yamada S, Kusano K, Baba S. Clinical potential and current progress of dental pulp stem cells for various systemic diseases in regenerative medicine: a concise review. International Journal of Molecular Sciences. 2019;20(5):1132. https://doi.org/10.3390/ijms20051132  
  3. Yamagata K, Onizawa K, Yanagawa T, Hasegawa Y, Kojima H, Nagasawa T, Yoshida H. A prospective study to evaluate a new dental management protocol before hematopoietic stem cell transplantation. Bone Marrow Transplantation. 2006;38(3):237-42. https://doi.org/10.1038/sj.bmt.1705429  
  4. Alinejad Y, Adoungotchodo A, Hui E, Zehtabi F, Lerouge S. An injectable chitosan/chondroitin sulfate hydrogel with tunable mechanical properties for cell therapy/tissue engineering. International journal of biological macromolecules. 2018;113:132-41. https://doi.org/10.1016/j.ijbiomac.2018.02.069  
  5. Haraguchi Y, Shimizu T, Yamato M, Okano T. Concise review: cell therapy and tissue engineering for cardiovascular disease. Stem Cells Translational Medicine. 2012;1(2):136-41. https://doi.org/10.5966/sctm.2012-0030  
  6. Senni K, Pereira J, Gueniche F, Delbarre-Ladrat C, Sinquin C, Ratiskol J, Godeau G, et al. Marine polysaccharides: a source of bioactive molecules for cell therapy and tissue engineering. Marine Drugs. 2011;9(9):1664-81. https://doi.org/10.3390/md9091664  
  7. Habib M, Shapira-Schweitzer K, Caspi O, Gepstein A, Arbel G, Aronson D, Seliktar D, et al. A combined cell therapy and in-situ tissue-engineering approach for myocardial repair. Biomaterials. 2011;32(30):7514-23. https://doi.org/10.1016/j.biomaterials.2011.06.049  
  8. Guo B, Qu J, Zhao X, Zhang M. Degradable conductive self-healing hydrogels based on dextran-graft-tetraaniline and N-carboxyethyl chitosan as injectable carriers for myoblast cell therapy and muscle regeneration. Acta Biomaterialia. 2019;84:180-93. https://doi.org/10.1016/j.actbio.2018.12.008  
  9. Marofi F, Hassanzadeh A, Solali S, Vahedi G, Mousavi Ardehaie R, Salarinasab S, Aliparasti MR, et al. Epigenetic mechanisms are behind the regulation of the key genes associated with the osteoblastic differentiation of the mesenchymal stem cells: The role of zoledronic acid on tuning the epigenetic changes. Journal of cellular physiology. 2019;234(9):15108-22. https://doi.org/10.1002/jcp.28152  
  10. Wang L-T, Liu K-J, Sytwu H-K, Yen M-L, Yen BL. Advances in mesenchymal stem cell therapy for immune and inflammatory diseases: Use of cell-free products and human pluripotent stem cell-derived mesenchymal stem cells. Stem cells translational medicine. 2021;10(9):1288-303. https://doi.org/10.1002/sctm.21-0021  
  11. Zhang K, Jiang Y, Wang B, Li T, Shang D, Zhang X. Mesenchymal stem cell therapy: A potential treatment targeting pathological manifestations of traumatic brain injury. Oxidative Medicine and Cellular Longevity. 2022;2022. https://doi.org/10.1155/2022/4645021  
  12. Meza G, Urrejola D, Saint Jean N, Inostroza C, López V, Khoury M, Brizuela C. Personalized cell therapy for pulpitis using autologous dental pulp stem cells and leukocyte platelet-rich fibrin: a case report. Journal of Endodontics. 2019;45(2):144-9. https://doi.org/10.1016/j.joen.2018.11.009  
  13. Nasirmoghadas P, Mousakhani A, Behzad F, Beheshtkhoo N, Hassanzadeh A, Nikoo M, Mehrabi M, et al. Nanoparticles in cancer immunotherapies: An innovative strategy. Biotechnology progress. 2021;37(2):e3070. https://doi.org/10.1002/btpr.3070  
  14. Bronckers A, Lyaruu D, DenBesten P. The impact of fluoride on ameloblasts and the mechanisms of enamel fluorosis. Journal of dental research. 2009;88(10):877-93. https://doi.org/10.1177/0022034509343280  
  15. Lacruz R, Smith C, Kurtz I, Hubbard M, Paine M. New paradigms on the transport functions of maturation-stage ameloblasts. Journal of dental research. 2013;92(2):122-9. https://doi.org/10.1177/0022034512470954  
  16. Fukumoto S, Kiba T, Hall B, Iehara N, Nakamura T, Longenecker G, Krebsbach PH, et al. Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts. The Journal of cell biology. 2004;167(5):973-83. https://doi.org/10.1083/jcb.200409077  
  17. Raposo G, Stahl PD. Extracellular vesicles: a new communication paradigm? Nature Reviews Molecular Cell Biology. 2019;20(9):509-10. https://doi.org/10.1038/s41580-019-0158-7  
  18. Hassanzadeh A, Rahman HS, Markov A, Endjun JJ, Zekiy AO, Chartrand MS, Beheshtkhoo N, et al. Mesenchymal stem/stromal cell-derived exosomes in regenerative medicine and cancer; overview of development, challenges, and opportunities. Stem Cell Research & Therapy. 2021;12(1):1-22. https://doi.org/10.1186/s13287-021-02378-7  
  19. Brunello G, Zanotti F, Trentini M, Zanolla I, Pishavar E, Favero V, Favero R, et al. Exosomes Derived from Dental Pulp Stem Cells Show Different Angiogenic and Osteogenic Properties in Relation to the Age of the Donor. Pharmaceutics. 2022;14(5). https://doi.org/10.3390/pharmaceutics14050908  
  20. Zhang S, Chuah SJ, Lai RC, Hui JHP, Lim SK, Toh WS. MSC exosomes mediate cartilage repair by enhancing proliferation, attenuating apoptosis and modulating immune reactivity. Biomaterials. 2018;156:16-27. https://doi.org/10.1016/j.biomaterials.2017.11.028  
  21. Teotia AK, Qayoom I, Singh P, Mishra A, Jaiman D, Seppälä J, Lidgren L, et al. Exosome-functionalized ceramic bone substitute promotes critical-sized bone defect repair in rats. ACS Applied Bio Materials. 2021;4(4):3716-26. https://doi.org/10.1021/acsabm.1c00311  
  22. Liu X, Li Q, Niu X, Hu B, Chen S, Song W, Ding J, et al. Exosomes secreted from human-induced pluripotent stem cell-derived mesenchymal stem cells prevent osteonecrosis of the femoral head by promoting angiogenesis. International journal of biological sciences. 2017;13(2):232. https://doi.org/10.7150/ijbs.16951
Nanomedicine Research Journal
Volume 8, Issue 2
Spring 2023
Pages 186-192