Saman Firoozi; Mohammad Ali Derakhshan; Roya Karimi; Ali Rashti; Babak Negahdari; Reza Faridi Majidi; Samaneh Mashaghi; Hossien Ghanbari
Abstract
Objective(s): Tissue engineering represents a new approach to solve the current complications of the heart valve replacements by offering viable valve prosthesis with growth and remodeling capability. In this project, electrospinning and dip coating techniques were used to fabricate heart valve constructs ...
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Objective(s): Tissue engineering represents a new approach to solve the current complications of the heart valve replacements by offering viable valve prosthesis with growth and remodeling capability. In this project, electrospinning and dip coating techniques were used to fabricate heart valve constructs from medical grade polyurethane (PU). Methods: First, a mold of tricuspid valve was dip coated in a PU solution, except for its valvular parts. Then, PU nanofibers were electrospun on the dip coated mold to form the valves. The morphology and diameter of nanofibers were investigated by SEM and contact angle measurements were done to evaluate the wettability of scaffolds. Thereafter, a tensile tester machine was used to assess mechanical properties of nanofibrous scaffolds. Then, the HUVEC cell line was cultured on the surface of scaffolds. Results: The SEM images showed the proper nanofibrous structure of the prepared scaffolds. Also, the obtained structure demonstrated appropriate tensile properties. Based on direct and indirect MTT, DAPI staining and SEM results, nanofibers were biocompatible and cells attached to the surface of the scaffolds, properly. Conclusions: This study demonstrated polyurethane-based nanofibrous scaffolds for engineering artificial heart valve. The presented scaffold provides temporary support for cells prior to generation of extracellular matrix (ECM).
Ali Gheibi; Kamyar Khoshnevisan; Najmeh Ketabchi; Mohammad Ali Derakhshan; Arman Amani Babadi
Abstract
Among the synthetic polymers, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) microbial polyester is one of the biocompatible and biodegradable copolymers in the nanomedicine scope. PHBV has key points and suitable properties to support cellular adhesion, proliferation and differentiation of nanofibers. ...
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Among the synthetic polymers, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) microbial polyester is one of the biocompatible and biodegradable copolymers in the nanomedicine scope. PHBV has key points and suitable properties to support cellular adhesion, proliferation and differentiation of nanofibers. Nanofibers are noticeably employed in order to enhance the performance of biomaterials, and could be effectively considered in this scope. Electrospinning is one of the well-known and practical methods that extremely employed in the construction of nanofibrous scaffolds for biomedical application and recently PHBV has exploited in nerve graft and regenerative medicine. PHBV composites nanofibrous scaffolds are able to be applied as promising materials in many fields, such as; wound healing and dressing, tissue engineering, targeted drug delivery systems, functional carries, biosensors or nano-biosensors and so on. In this mini-review, we attempt to provide a more detailed overview of the recent advances of PHBV electrospun nanofibers application in neural graft and regeneration.
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