Atefeh Hekmat; Hossein Attar; Ali Akbar Seifkordi; Maryam Iman; Mahmoud Reza Jaafari
Abstract
Poor solubility, low bio-stability, and the high toxicity of docetaxel medicine result in limited consumption, common side effects, and low efficacy. The current commercial form of the product, Taxotere®, with intravenous administration caused hypersensitivity due to hemolysis by separating hemoglobin ...
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Poor solubility, low bio-stability, and the high toxicity of docetaxel medicine result in limited consumption, common side effects, and low efficacy. The current commercial form of the product, Taxotere®, with intravenous administration caused hypersensitivity due to hemolysis by separating hemoglobin and red blood cells. In addition, the patient suffers from a severe treatment regime through prolonging medicine injection. The most important advantages of medicine in cancer treatment are to consider patients' comfort, and acceptance during treatment, as well as to choose the most effective medicine to achieve the highest improvement in cancer cells. Following our previous study, in this study stabilized docetaxel loaded nanomicelles were used for the treatment of mice with C26 colon carcinoma. The synthesized nanomicelles have satisfying results on animal trials and adequate characters such as an oral form of medicine, particle size of less than 15 nm, proper PDI, sufficient zeta potential for physical stability and maintaining particle size, non-toxicity of career, and high efficacy than the commercial product Taxotere®. In addition, lower side effects of synthesized oral form medicine on the treatment of C26 colon carcinoma can be named as the other advantage of this study.
Elham Zendedel; Fatemeh Gheybi; Jamshidkhan Chamani; Mahmoud Reza Jaafari
Abstract
Abstract Curcumin, which is derived from the turmeric rhizomes (curcuma longa) as a natural polyphenol, is a substantially lipophilic molecule. This commonly used substance is employed as a spice and coloring agent in food and contains potent antioxidant, as well as anti-inflammatory, and anti-proliferative ...
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Abstract Curcumin, which is derived from the turmeric rhizomes (curcuma longa) as a natural polyphenol, is a substantially lipophilic molecule. This commonly used substance is employed as a spice and coloring agent in food and contains potent antioxidant, as well as anti-inflammatory, and anti-proliferative tumor activities. The developed nanomicelle formulations of curcumin are used to promote the bio-availability and solubility of the above-mentioned lipophilic molecule. The present investigation aimed to examine the anti-proliferative activity of nanomicelle and free curcuminoids by using different cancer and normal cells using a tetrazolium dye-based assay. To this end, various cell lines were treated with nanomicelle or free curcuminoids at different concentration of 5, 10, 20, 30, and 40 µM for 48 h at 37 ºC. Our results demonstrated that the half maximal inhibitory concentrations of the micellar form of curcuminoids for different cancer cell lines were as high as its levels measured for its free form but in normal cells, the toxicity of nanomicelles is lower than free form of curcuminoids.
Nahid Fakhraei; Reza Hashemibakhsh; Seyed Mahdi Rezayat; Amir Hossein Abdolghafari; Mahmoud Reza Jaafari; Faiza Mumtaz; Seyedeh Ellaheh Mousavi
Abstract
Objectives: Cardiotoxicity is considered the primary cause of death in aluminum phosphide (ALP)-poisoned cases. Curcumin, the active ingredient of turmeric, is a potent protective polyphenol compound against cardiac diseases including cardiotoxicity. This study aimed to examine the probable cardioprotection ...
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Objectives: Cardiotoxicity is considered the primary cause of death in aluminum phosphide (ALP)-poisoned cases. Curcumin, the active ingredient of turmeric, is a potent protective polyphenol compound against cardiac diseases including cardiotoxicity. This study aimed to examine the probable cardioprotection potential of nanocurcumin in a rat model of ALP-induced cardiotoxicity. Methods: The rats were orally intoxicated with ALP (12 mg/kg, p.o.; 1/4 LD50). In treatment groups, curcumin (50 mg/kg, i.p.) and nanocurcumin (10, 20 and 50 mg/kg, i.p.) were administered intraperitoneally 30 min following ALP administration. Twenty four hrs subsequent to ALP intoxication, the hearts were dissected out for evaluation of oxidative stress and lipid peroxidation (LPO) markers such as thiol, reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione (GSH) levels, malondialdehyde (MDA) and the ferric reducing ability of plasma (FRAP). Findings: In fact, ALP increased MDA as well as ROS and SOD levels. On the other hand, ALP significantly lowered thiol, GHS and FRAP markers. In contrast, nanocurcumin successfully could reverse the increases in MDA as well as SOD, ROS and GSH. Simultaneously, it significantly enhanced thiol, GHS and FRAP markers. Moreover, curcumin markedly lowered MDA, ROS and SOD levels while increased thiol and GSH contents. Conclusion: Overall, the present data demonstrated the cardio-protective effects of nanocurcumin in this model of cardiotixicity. Further, it suggested that this cardioprotecton is probably mediated by the ability of nanocurcumin to confront the oxidative stress and LPO resulting from ALP intoxication of the heart tissue. Key words: Nanocurcumin; aluminum phosphide; cardiotoxicity; oxidative stress; rat.
Fatemeh Gheybi; Seyedeh Hoda Alavizadeh; Seyed Mahdi Rezayat; Elham Zendedel; Mahmoud Jaafari
Abstract
Cancer is the second leading cause of death worldwide. Despite great efforts over many years, today cancer treatment is not very effective. The main reasons for cancer chemotherapy failure are high cytotoxicity, low response rates in solid tumors, and development of resistance. Different experimental ...
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Cancer is the second leading cause of death worldwide. Despite great efforts over many years, today cancer treatment is not very effective. The main reasons for cancer chemotherapy failure are high cytotoxicity, low response rates in solid tumors, and development of resistance. Different experimental studies have shown that drug combination using low toxicity natural compounds such as polyphenols can reduce the required dose of cytotoxic drugs for cancer treatment. The polyphenolic compound, Silymarin (SLM), is an active extract from the seeds of the plant milk thistle (Silybum Marianum). It is well known for its hepatoprotective, antioxidant and chemoprotective effects. In present study, we investigated cytotoxic effects of combined liposomal doxorubicin (DXL) and SLM on 4T1 breast cancer cells at different molar ratios of the two drugs and we focused on elucidating whether the combination of the two drugs could dictate antitumor activity in vitro. Results indicated that SLM-DXL combination at 100 and 300 molar ratios, exert synergistic growth-inhibitory effects. These synergistic effects were observed only at lower SLM-DXL concentrations. In conclusion, it is conceivable that in SLM-DXL combination chemotherapy, drug ratios play a key role which determine the final response following treatment. Thus, using liposomes as targeted drug delivery systems, it would be possible to achieve appropriate combination of the two drugs at correct doses and correct administration intervals clinically. Keywords: Silymarin; Doxorubicin; Liposomes; Combination therapy; 4T1
