Elham Zendedel; Fatemeh Gheybi; Jamshidkhan Chamani; Mahmoud Reza Jaafari
Abstract
Abstract Curcumin, which is derived from the turmeric rhizomes (curcuma longa) as a natural polyphenol, is a substantially lipophilic molecule. This commonly used substance is employed as a spice and coloring agent in food and contains potent antioxidant, as well as anti-inflammatory, and anti-proliferative ...
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Abstract Curcumin, which is derived from the turmeric rhizomes (curcuma longa) as a natural polyphenol, is a substantially lipophilic molecule. This commonly used substance is employed as a spice and coloring agent in food and contains potent antioxidant, as well as anti-inflammatory, and anti-proliferative tumor activities. The developed nanomicelle formulations of curcumin are used to promote the bio-availability and solubility of the above-mentioned lipophilic molecule. The present investigation aimed to examine the anti-proliferative activity of nanomicelle and free curcuminoids by using different cancer and normal cells using a tetrazolium dye-based assay. To this end, various cell lines were treated with nanomicelle or free curcuminoids at different concentration of 5, 10, 20, 30, and 40 µM for 48 h at 37 ºC. Our results demonstrated that the half maximal inhibitory concentrations of the micellar form of curcuminoids for different cancer cell lines were as high as its levels measured for its free form but in normal cells, the toxicity of nanomicelles is lower than free form of curcuminoids.
Fatemeh Gheybi; Seyedeh Hoda Alavizadeh; Seyed Mahdi Rezayat; Elham Zendedel; Mahmoud Jaafari
Abstract
Cancer is the second leading cause of death worldwide. Despite great efforts over many years, today cancer treatment is not very effective. The main reasons for cancer chemotherapy failure are high cytotoxicity, low response rates in solid tumors, and development of resistance. Different experimental ...
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Cancer is the second leading cause of death worldwide. Despite great efforts over many years, today cancer treatment is not very effective. The main reasons for cancer chemotherapy failure are high cytotoxicity, low response rates in solid tumors, and development of resistance. Different experimental studies have shown that drug combination using low toxicity natural compounds such as polyphenols can reduce the required dose of cytotoxic drugs for cancer treatment. The polyphenolic compound, Silymarin (SLM), is an active extract from the seeds of the plant milk thistle (Silybum Marianum). It is well known for its hepatoprotective, antioxidant and chemoprotective effects. In present study, we investigated cytotoxic effects of combined liposomal doxorubicin (DXL) and SLM on 4T1 breast cancer cells at different molar ratios of the two drugs and we focused on elucidating whether the combination of the two drugs could dictate antitumor activity in vitro. Results indicated that SLM-DXL combination at 100 and 300 molar ratios, exert synergistic growth-inhibitory effects. These synergistic effects were observed only at lower SLM-DXL concentrations. In conclusion, it is conceivable that in SLM-DXL combination chemotherapy, drug ratios play a key role which determine the final response following treatment. Thus, using liposomes as targeted drug delivery systems, it would be possible to achieve appropriate combination of the two drugs at correct doses and correct administration intervals clinically. Keywords: Silymarin; Doxorubicin; Liposomes; Combination therapy; 4T1
