Fatemeh Madani; Basil Mujokoro; Shima Mohammadi; Masood Khosravani; Mahdi Adabi
Abstract
In this study, we prepared methotrexate (MTX) loaded chitosan/polyvinyl alcohol (PVA) nanofibers using electrospinning method. The prepared nanofiber mats were soaked into the glutaraldehyde solution for crosslinking. The ratio of chitosan/PVA was 1:9 and 1:7, and the crosslinking time was 24 hrs and ...
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In this study, we prepared methotrexate (MTX) loaded chitosan/polyvinyl alcohol (PVA) nanofibers using electrospinning method. The prepared nanofiber mats were soaked into the glutaraldehyde solution for crosslinking. The ratio of chitosan/PVA was 1:9 and 1:7, and the crosslinking time was 24 hrs and 36 hrs. In vitro release study was performed on four formulations of nanofibers. Determination of contact angle, SEM, and MTT assay on U-87 MG cell line and MCF-7 cell line were carried out by those formulations of nanofibers. SEM images showed that the average fiber diameter was 221 nm with a range of 94–410 nm and also water contact angle was 24.3o. In vitro release profile of nanofibers with the ratio of chitosan/PVA of 1:9 and crosslinking time of 36 hrs was more acceptable compared to other ones. After 24 hrs, Chitosan/PVA nanofibers mat containing MTX had 18% and 20% cytotoxicity on U87MG and MCF7 cell lines, respectively. In conclusion, MTX loaded chitosan/PVA nanofibers would be an appropriate therapeutics implant for cancer therapy; however, more studies are also needed.
samira samadieh; minoo sadri
Abstract
Objective(s): Biodegradable polymers are featured with notable potentials for biotechnology and bioengineering purposes. Still, there are limitations in their applicability so that in many cases composite forms are used. The present study is focused on chitosan (CS), gelatin (GEL), honey (H) and aloe ...
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Objective(s): Biodegradable polymers are featured with notable potentials for biotechnology and bioengineering purposes. Still, there are limitations in their applicability so that in many cases composite forms are used. The present study is focused on chitosan (CS), gelatin (GEL), honey (H) and aloe vera (AV) for preparation of thin films.Methods: To prepare the thin film, CS and GEL with ratio of 8 % was used. Similarly, AV, honey and wheat germ oil (WGO) was prepared with 20:20:2 ratio. Afterward, the solution was poured in petri dishes. The dishes were stored at room temperature for 24h until the film was formed. Then, CS/PEO/H, CS/PEO/AV, CS/PEO/H/AV nanofibers electrospinning was done on CS/GEL/H, CS/GEL/AV, CS/GEL/H/AV thin films, respectively. Results: The results of antibacterial activity, fibroblast cells culture and hemolytic activity were examined. The results of antibacterial tests revealed that thin films containing honey had antibacterial activity against staphylococcus aureus and pseudomonas aeruginosa. The results of fibroblast cells on the prepared samples indicated more than 90% of the cells are alive. Also,hemolytic activity results indicate that the samples are non-hemolytic.Conclusions: Considering the overall physiochemical and biological properties of the thin films, they could be advantageously used as a promising biomaterial for antibacterial coating.
Farnaz Nayeb morad; Abosaeed Rashidi; Ramin Khajavi; Mohammad karim Rahimi; Abbas Bahador
Abstract
Gum Tragacanth (GT) obtained from Astragalus Gossypinus is one of the most widely used natural gums which has found applications in many areas because of its attractive features such as biodegradability, nontoxic nature, natural availability, moisture absorption and creating a network of Hydrocolloid. ...
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Gum Tragacanth (GT) obtained from Astragalus Gossypinus is one of the most widely used natural gums which has found applications in many areas because of its attractive features such as biodegradability, nontoxic nature, natural availability, moisture absorption and creating a network of Hydrocolloid. It also has maintenance and delivery of drugs, higher resistance to microbial attacks and long shelf-life properties.In present study, preparation nanofibers of 50 wt% Bassorin (extracted from Gum Tragacanth) has been mixed by 50 wt% Poly Ethylene Oxide and 0.01 wt% Ofloxacin (Ba/PEO/Ofx) for Electrospinning. Nanofibers coated on cotton gauze. The properties of Bassorin and produced nanofibers were examined via XRD, FTIR and SEM microscopy. The Antibacterial of nanofibers activity against Staphylococcus aureus as gram positive bacteria and Escherichia coli as a gram-negative bacteria also were investigated. Nanofibers are capable of absorbing wound’s exocrine liquid easily due to their high specific area of nanofibers which 4 to 5% more than cotton gauzes without nanofibers. When it is turned to gel by moisture sorption, the release of loaded Ofloxacin would be enhanced. The Antibacterial assay showed the cotton gauze coated with Ba/PEO/Ofx nanofibers could inhibit about 90% growth both bacterial strain on burn wound. Also, the therapeutic effect of nano-bassorin in restoring superficial second-degree burns in rats showed an accelerated effect on wound healing.Based on the results of this study, it is possible to use cotton gauzes coated with bassorin nanofibers as a suitable candidate for the treatment of second-degree superficial burns.
Seyedeh Sara Esnaashari; Majid Naghibzadeh; Mahdi Adabi; Reza Faridi Majidi
Abstract
Objective(s): This paper investigates the validity of Artificial Neural Networks (ANN) model in the prediction of electrospun kefiran nanofibers diameter using 4 effective parameters involved in electrospinning process. Polymer concentration, applied voltage, flow rate and nozzle to collector distance ...
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Objective(s): This paper investigates the validity of Artificial Neural Networks (ANN) model in the prediction of electrospun kefiran nanofibers diameter using 4 effective parameters involved in electrospinning process. Polymer concentration, applied voltage, flow rate and nozzle to collector distance were used as variable parameters to design various sets of electrospinning experiments for production of electrospun kefiran nanofibers. Methods: The Scanning Electron Microscopy (SEM) was used to investigate the morphology and evaluate the size of the nanofiber. Data set was drawn using k fold cross-validation method, which was the most suitable scheme for the volume of the data in this work. Data were partitioned into the five series and trained and tested via ANN method. Results: The Scanning Electron Microscopy (SEM) images of the generated nanofiber samples were confirmed that all of the samples were fine and defect-free. Our results indicated that the network including four input variables, three hidden layers with 10, 18 and 9 nodes in each layer, respectively, and one output layer obtained the highest efficiency in the testing set. The mean squared error (MSE) and linear regression (R) between observed and predicted nanofibers diameter were 0.0452 and 0.950, respectively. Conclusions: The results demonstrated that the proposed neural network was appropriately performed in assessing the input parameters and prediction of nanofibers diameter.
Minoo Sadri; Saede Arab Sorkhi
Abstract
Objective(S): Electrospinning of chitosan/polyethylene oxide (CS/PEO) nanofibers with the addition of cefazolin to create nanofibers with antimicrobial properties were examined. Methods: Polymeric nanofibers including CS/PEO and CS/PEO /cefazolin, were produced by electrospinning method. The range of ...
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Objective(S): Electrospinning of chitosan/polyethylene oxide (CS/PEO) nanofibers with the addition of cefazolin to create nanofibers with antimicrobial properties were examined. Methods: Polymeric nanofibers including CS/PEO and CS/PEO /cefazolin, were produced by electrospinning method. The range of nanofiber was 60-100 nm in diameter and measured with ImageJ software. The morphology of electrospun nanofibers was studied with use of scanning electron microscopy (SEM). Moreover, the chemical structures of the nanofibers were evaluated by FT-IR. The drug release of nanofibers was also investigated by UV-Vis spectrophotometry. The antibacterial activity of scaffolds was tested by two type bacteria including Escherichia coli and Staphylococcus aureus. The healing ability of nanofibers was studied on the rat’s wound. Results: The SEM images indicated that the addition of cefazolin as much as 1wt% brings about the best nanofiber. Also, the morphology of electrospun nanofiber is dependent on the viscosity of the solution and the ratio of CS /PEO/cefazolin. According to the results of cefazolin releasing from nanofibers, the best results were obtained in the presence of CS /PEO/1wt%cefazolin nanofibers as healing sample. In animal studies, the effect of nanofibers was studied in the burn wound healing of rats and improvement of the wound was observed by nanofibers containing 1%wt cefazolin. Conclusions: According to these results, it seems that CS /PEO/1wt% cefazolin nanofiber is a good choice as a wound covering agent and hold more moisture in its structure thus the surface of wound remain wet during the healing process that prevent from nanofiber sticking to the wound surface.
Saman Firoozi; Mohammad Ali Derakhshan; Roya Karimi; Ali Rashti; Babak Negahdari; Reza Faridi Majidi; Samaneh Mashaghi; Hossien Ghanbari
Abstract
Objective(s): Tissue engineering represents a new approach to solve the current complications of the heart valve replacements by offering viable valve prosthesis with growth and remodeling capability. In this project, electrospinning and dip coating techniques were used to fabricate heart valve constructs ...
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Objective(s): Tissue engineering represents a new approach to solve the current complications of the heart valve replacements by offering viable valve prosthesis with growth and remodeling capability. In this project, electrospinning and dip coating techniques were used to fabricate heart valve constructs from medical grade polyurethane (PU). Methods: First, a mold of tricuspid valve was dip coated in a PU solution, except for its valvular parts. Then, PU nanofibers were electrospun on the dip coated mold to form the valves. The morphology and diameter of nanofibers were investigated by SEM and contact angle measurements were done to evaluate the wettability of scaffolds. Thereafter, a tensile tester machine was used to assess mechanical properties of nanofibrous scaffolds. Then, the HUVEC cell line was cultured on the surface of scaffolds. Results: The SEM images showed the proper nanofibrous structure of the prepared scaffolds. Also, the obtained structure demonstrated appropriate tensile properties. Based on direct and indirect MTT, DAPI staining and SEM results, nanofibers were biocompatible and cells attached to the surface of the scaffolds, properly. Conclusions: This study demonstrated polyurethane-based nanofibrous scaffolds for engineering artificial heart valve. The presented scaffold provides temporary support for cells prior to generation of extracellular matrix (ECM).
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