Saba Talesh; Mohammad Koohi; Ehsan Zayerzadeh; Jalal Hasan; Meisam Shabanian
Abstract
Iron oxide nanoparticles (IONPS) have potential different applications in nanomedicine. These new applications of IONPS have raised exposure risk of this nanomaterials to humans. Up to now, all aspects of IONPS toxicity are not fully clear following animal’s exposure with these novel compounds. ...
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Iron oxide nanoparticles (IONPS) have potential different applications in nanomedicine. These new applications of IONPS have raised exposure risk of this nanomaterials to humans. Up to now, all aspects of IONPS toxicity are not fully clear following animal’s exposure with these novel compounds. This study aimed to investigate the acute toxicity effects of IONPS in laboratory animals regarding pathotoxicological analysis and clinical aspects. Twenty four male Wistar rats were randomly chosen, and divided into four groups of six rats each. The first, second, and the third groups received 50, 500, and 5000 mg/kg of IONPS solution orally for five days through gavage, respectively. Animal mortality, clinical sings and body weight were evaluated during the study. Fourteen days after the last administration, rats were euthanized for further investigation for histopathological evaluation. There were no death observed in all groups. High and middle dose of the IONPS caused symptoms like lethargy, ataxia, anorexia, isolation, and respiratory arrhythmia over the period of the study. The subjects of the low dose group showed no signs of toxicity. Specific histopathological complications, like hyaline cast in the kidneys, hyperemia and interstitial thickening in the lungs, hemorrhage in the heart and hepatic degeneration in the liver were observed in high dose group. Thus, it can be concluded that, toxicity of IONPS in rats is dose-dependent. This particular size of IONPS can induce serious pathological abnormalities and clinical symptoms in high dose.
Mahnaz Kesmati; Mozhgan Torabi; Nahid Pourreza; Mehrnaz Tayebkhah; Fereshteh Asadi
Abstract
Objective(s): Based on our previous studies about nano-ZnO effects on physiological behaviors, in this study we have investigated the effects of anxiolytic doses of nano-ZnO on electrocardiogram (ECG) sings changes in the restraint and non-restraint ovariectomized (OVX) female rats. Methods: Animals ...
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Objective(s): Based on our previous studies about nano-ZnO effects on physiological behaviors, in this study we have investigated the effects of anxiolytic doses of nano-ZnO on electrocardiogram (ECG) sings changes in the restraint and non-restraint ovariectomized (OVX) female rats. Methods: Animals (160-180 g) were divided into: control (non-OVX + saline) and OVX groups that received: saline, nano-ZnO 1, 2.5 and 5 mg/kg, stress of 90 or 180 min + saline and stress of 180 min+ nano-ZnO 2.5 mg/kg. Anxiety-like behaviors were measured by the elevated plus maze (EPM) and hole board apparatus thirty min after intraperitoneally injections or stress induction. ECG parameters and serum zinc level were measured in all groups. Results: Ovariectomy induced anxiety and restraint stress in OVX rats increased it in the hole board test. Nano-ZnO 2.5 and 5 mg/kg improved anxiety-like behaviors in the EPM test. Nano-ZnO 2.5 mg/kg improved anxiety induced by the restraint stress in the hole board test. Nano-ZnO increased zinc level in a dose-dependent manner in the non-restraint OVX groups. Nano-ZnO 1 mg/kg decreased QRS amplitude and all doses decreased QT interval to the level of non-OVX group. Nano-ZnO 5 mg/kg decreased QTc to the level of non-OVX group. Stress of 90 min increased QT interval while stress of 180 min decreased it. Nano-ZnO after stress induction could alleviate heart rate, R-R- interval and QRS interval to the level of non-OVX group. Conclusion: It seems that nano-ZnO rather than could improve anxiety, alleviated ECG parameters in the restraint and non-restraint OVX rats.
Nahid Fakhraei; Reza Hashemibakhsh; Seyed Mahdi Rezayat; Amir Hossein Abdolghafari; Mahmoud Reza Jaafari; Faiza Mumtaz; Seyedeh Ellaheh Mousavi
Abstract
Objectives: Cardiotoxicity is considered the primary cause of death in aluminum phosphide (ALP)-poisoned cases. Curcumin, the active ingredient of turmeric, is a potent protective polyphenol compound against cardiac diseases including cardiotoxicity. This study aimed to examine the probable cardioprotection ...
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Objectives: Cardiotoxicity is considered the primary cause of death in aluminum phosphide (ALP)-poisoned cases. Curcumin, the active ingredient of turmeric, is a potent protective polyphenol compound against cardiac diseases including cardiotoxicity. This study aimed to examine the probable cardioprotection potential of nanocurcumin in a rat model of ALP-induced cardiotoxicity. Methods: The rats were orally intoxicated with ALP (12 mg/kg, p.o.; 1/4 LD50). In treatment groups, curcumin (50 mg/kg, i.p.) and nanocurcumin (10, 20 and 50 mg/kg, i.p.) were administered intraperitoneally 30 min following ALP administration. Twenty four hrs subsequent to ALP intoxication, the hearts were dissected out for evaluation of oxidative stress and lipid peroxidation (LPO) markers such as thiol, reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione (GSH) levels, malondialdehyde (MDA) and the ferric reducing ability of plasma (FRAP). Findings: In fact, ALP increased MDA as well as ROS and SOD levels. On the other hand, ALP significantly lowered thiol, GHS and FRAP markers. In contrast, nanocurcumin successfully could reverse the increases in MDA as well as SOD, ROS and GSH. Simultaneously, it significantly enhanced thiol, GHS and FRAP markers. Moreover, curcumin markedly lowered MDA, ROS and SOD levels while increased thiol and GSH contents. Conclusion: Overall, the present data demonstrated the cardio-protective effects of nanocurcumin in this model of cardiotixicity. Further, it suggested that this cardioprotecton is probably mediated by the ability of nanocurcumin to confront the oxidative stress and LPO resulting from ALP intoxication of the heart tissue. Key words: Nanocurcumin; aluminum phosphide; cardiotoxicity; oxidative stress; rat.
Elham Salami; Manizheh Karami; Ameneh Jafaryan Dehkordi; Mohammadreza Jalali Nadoushan; Abazar Hajnorouzi
Abstract
Background and Objective: Morphine can cause harmful effects in the ovaries. The silver nanoparticles (Ag-NPs) used in health care because of antimicrobial properties, can diffuse into the brain blood barrier. This study investigated the protective effect of Ag-NPs on the induction of polycystic ovary ...
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Background and Objective: Morphine can cause harmful effects in the ovaries. The silver nanoparticles (Ag-NPs) used in health care because of antimicrobial properties, can diffuse into the brain blood barrier. This study investigated the protective effect of Ag-NPs on the induction of polycystic ovary (PCO) due to injection of morphine intra-ventromedial hypothalamus (intra-VMH) of rat compared with the animal receiving the drug (10-100 mg/kg) intraperitoneally (i.p.) twice daily for 7 days to lead to addiction. Materials and Methods: The rats (bought of Pasture Institute of Iran weighing 200-250 g) were housed under standard conditions and fed ad libitum. They were randomly divided to addict to drug or morphine (0.001 to 0.4 μg/rat) receiving into the VMH (AP: -1.92) a week after stereotaxic surgery. Ag-Nps (0.01, 0.001 and 0.0001 μg/rat) were administered intra-VMH alone or prior to morphine effective dose (0.4 μg/rat). Control group was given only saline. By the end of the treatment, the animals’ ovaries and/or brain were dissected and studied histopathologically. The ovaries were also checked by the marker of the NO, NADPH-diaphorase. Results: All experimental rats’ ovaries treated morphine showed polycystic characteristics and the NO activation was evidenced in the ovaries in the comparison with the saline group (p
Zohreh Parang; Davood Moghadamnia
Abstract
Objective(s): Silver nanoparticles show anti-fungal properties, and is widely used in medicine. In this research, the impacts of silver nanoparticles on the hepatic functional tests and changes in liver tissues in adult male rats were investigated.
Methods: In this experimental study, 28 adult ...
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Objective(s): Silver nanoparticles show anti-fungal properties, and is widely used in medicine. In this research, the impacts of silver nanoparticles on the hepatic functional tests and changes in liver tissues in adult male rats were investigated.
Methods: In this experimental study, 28 adult male Wistar rats, each weighing approximately 180-220 g were divided into 4 groups of 7: the control group, and the experimental groups 1 and 2 received silver nanoparticles that were synthesized at 75 seconds interval with doses of 25 and 100 mg/kg intraperitoneally for 14 days, respectively. Experimental group 3 received silver nanoparticles that were synthesized at 300 seconds interval with a dose of 25 mg/kg intraperitoneally for 14 days. At the end of experiment period, blood samples were obtained from their hearts, and serum levels of hepatic enzymes (AST, ALT, ALP), albumen and total protein were measured. In addition, possible histological changes in liver was studied after hematoxylin-eosin staining. The results were statistically analyzed using ANOVA and Duncan test.
Results: The findings reported that the mean serum levels of aspartate aminotransferase (AST), total Protein and albumin in the experimental groups 1 and 3 increased significantly relative to the control group. Similarly, the mean serum levels of alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in the experimental group 3 increased significantly relative to the control group (P < 0.05). Also, necrosis of the liver tissue was observed in the recipients of silver nanoparticles.
Conclusions: The use of silver nanoparticles can boost the serum levels of hepatic enzymes and Increase liver tissue necrosis as well.
