nanomedicine
Aminatun - -; Ilham Alif Furqon; Dyah Hikmawati; Che Azurahanim Che Abdullah
Abstract
Objective(s) : The probability of contracting an infection when implanting an Stainless Steel 316L (SS316L) implant has been increasing. Infection due to implant placement is called osteomyelitis which is bone inflammation caused by biofilms formed by pyogenic bacteria. Biofilms can be prevented by giving ...
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Objective(s) : The probability of contracting an infection when implanting an Stainless Steel 316L (SS316L) implant has been increasing. Infection due to implant placement is called osteomyelitis which is bone inflammation caused by biofilms formed by pyogenic bacteria. Biofilms can be prevented by giving antibacterial agents. This study aims to examine the potential of silver nanoparticles (AgNPs) as an antibacterial agent in SS316L implants. Methods : AgNPs are made through a chemical synthesis process using the Gallic acid reduction method. AgNPs solution with 5 variations of precursor concentration, namely 0.1 mM, 1 mM, 10 mM, 100 mM each added with gelatin was sprayed on SS316L by the airbrush spray coating method with a distance between nozzle and substrate of 20 cm at a pressure of 40 psi. Result : AgNPs solutions produced from various concentrations of AgNO3 precursors have a range of λmax = 401.5 nm- 424.5 nm and a particle size distribution of 0.97 - 4.88 nm. The AgNPs layer on SS316L was characterized by its crystalline phase, crystal size, and anti-bacterial activity. It has a cubic structure with a phase fraction of 6.5-19%. Based on the antibacterial activity test, all AgNPs layer samples had inhibitory zone diameters in the range of 12-16 mm. AgNPs (10mM) + Gelatin layer showed the best antibacterial ability with an inhibitory zone diameter of 16.63 mm.Conclusion : The variation in the concentration of the 10 mM AgNPs precursor-Gelatin can be developed into a coating on the surface of the SS316L implant material.
Anoushe Raesian; Sepideh Arbabi Bidgoli; Seyed Mahdi Rezayat Sorkhabadi
Abstract
Objective(s): Silver nanoparticles have been widely used as new potent antimicrobial agents in cosmetic and hygienic products, as well as in new medical devices. Serious concerns have been expressed on the potential health risks of dermal applications of nanosilver containing consumer products (AgNPs), ...
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Objective(s): Silver nanoparticles have been widely used as new potent antimicrobial agents in cosmetic and hygienic products, as well as in new medical devices. Serious concerns have been expressed on the potential health risks of dermal applications of nanosilver containing consumer products (AgNPs), therefore regulatory health risk assessment has become necessary for the safe usage of AgNPs in biomedical products with special emphasis to their dermal toxicity potentials. We aimed in the present study to compare the dermal toxicity of three different AgNP containing disinfectantsin an albino rabbit model and tried to determine the role of size and other physicochemical properties on their possible dermal toxicity. Methods: After the characterization of all three samples by transmission electron microscopy (TEM), X-Ray Diffraction (XRD) and Dynamic Light Scattering (DLS) , corrosive and irritant potentials of AgNPs in three different sizes of three colloidal AgNPs were scored by the OECD 404 guideline with necessary modifications and were applied under the specified concentrations via nanosilver skin patches on the shaved skin of young female albino rabbits. All skin reactions were recorded in 3 min as well as in 1, 4, 24, 48 and 72 hours from the application and compared with the control group and followed up for 14 days. Results: Although short-term observations didn’t show any significant changes in the weight of animals and macroscopic variables, long-term histopathological abnormalities were seen in the skin of all test groups, which was not associated with the size and other physicochemical properties of AgNP samples. The toxicity manifestations were dry skin, scaling in doses lower than 100 ppm and erythema in higher doses up to 4000 ppm which was reversed. Conclusions: This finding creates a new issue in the possible dermal effects of all colloidal AgNPs, containing nano health products, which should be considered in future studies by focusing on other physicochemical properties of AgNPs and possible underlying mechanisms of toxicity by conducting cellular models.
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