Synthesis of Silver Oxide Nanoparticles using Different Precursor and Study Cytotoxicity Against MCF-7 Breast Cancer Cell line

Document Type : Original Research Article

Authors

1 Department of Chemistry, College of Science, University of Mustansiriyah, Baghdad, Iraq

2 Department of Chemistry, College of Education for Pure Science, University of Diyala, Baqubah, Diyala, Iraq

Abstract

In this work, the eco-friendly synthesis of silveroxidenanoparticles (Ag2O-NPs) was successfully performed using orange leaf extract with silver nitrate and silver sulfate as precursors. Several techniques were used to characterize the surfaces of the silver oxide nanoparticles (Ag2O-NPs), including FT-IR spectra, which showed the functional groups present in the synthesis of silver oxide nanoparticles, FE-SEM images showed that the green synthesis of Ag2O-NO3-NPs and Ag2O-SO4-NPs has a spherical shape. EDX analysis spectrum confirmed the presence of elemental silver signals in the Ag2O-NPs, proving that the Ag2O-NPs were prepared within the nanoscale range and that there is a clear and tangible effect of negative ions bonded (NO3- and SO4-2) with Ag metal on the size, shape, and distribution of Ag2O-NPs. Also, the cytoxicity of the established Ag NPs at 0-200 µg/ml was assessed in breast cancer MCF-7 cell lines in vitro using MTT assay, and verified these strong cytotoxicity. As a result, the use of orange leaf extract is considered an eco-friendly and cost-effective method.

Keywords

Main Subjects

References

  1. Shume, W. M., Murthy, H., & Zereffa, E. A. (2020). A review on synthesis and characterization of Ag2O nanoparticles for photocatalytic applications. Journal of Chemistry. https://doi.org/10.1155/2020/5039479
  2. Danish, M.S.S., et al., Photocatalytic applications of metal oxides for sustainable environmental remediation. Metals, 2021. 11(1): p. 80. https://doi.org/10.3390/met11010080  
  3. Torabi, S., et al., Synthesis, medical and photocatalyst applications of nano-Ag2O. Journal of Coordination Chemistry, 2020. 73(13): p. 1861-1880. https://doi.org/10.1080/00958972.2020.1806252  
  4. Huang, K., et al., Recent advances on silver-based photocatalysis: Photocorrosion inhibition, visible-light responsivity enhancement, and charges separation acceleration. Separation and Purification Technology, 2022. 283: p. 120194. https://doi.org/10.1016/j.seppur.2021.120194  
  5. Kiani, F.A., U. Shamraiz, and A. Badshah, Enhanced photo catalytic activity of Ag2O nanostructures through strontium doping. Materials Research Express, 2020. 7(1): p. 015035. https://doi.org/10.1088/2053-1591/ab608c  
  6. Al-Sarray, A.J., I.M. H. Al-Mousawi, and T. H. Al-Noor, Acid Activation of Iraqi Bentonite Clay: Its Structural, Dielectric and Electrical Behavior at Various Temperatures. Chemical Methodologies, 2022. 6(4): p. 331-338.  
  7. Reddy, P.N., et al., Characterization of silver oxide films formed by reactive RF sputtering at different substrate temperatures. International Scholarly Research Notices, 2014. 2014. https://doi.org/10.1155/2014/684317  
  8. Barhoum, A., et al., Recent trends in nanostructured particles: synthesis, functionalization, and applications, in Fundamentals of Nanoparticles. 2018, Elsevier. p. 605-639. https://doi.org/10.1016/B978-0-323-51255-8.00024-0  
  9. Yousaf, H., et al., Green synthesis of silver nanoparticles and their applications as an alternative antibacterial and antioxidant agents. Materials Science and Engineering: C, 2020. 112: p. 110901. https://doi.org/10.1016/j.msec.2020.110901  
  10. Rafique, M., et al., A review on green synthesis of silver nanoparticles and their applications. Artificial cells, nanomedicine, and biotechnology, 2017. 45(7): p. 1272-1291. https://doi.org/10.1080/21691401.2016.1241792  
  11. Castillo-Henríquez, L., et al., Green synthesis of gold and silver nanoparticles from plant extracts and their possible applications as antimicrobial agents in the agricultural area. Nanomaterials, 2020. 10(9): p. 1763. https://doi.org/10.3390/nano10091763  
  12. Ahmed, R.H. and D.E. Mustafa, Green synthesis of silver nanoparticles mediated by traditionally used medicinal plants in Sudan. International Nano Letters, 2020. 10(1): p. 1-14. https://doi.org/10.1007/s40089-019-00291-9  
  13. Rastogi, A., et al., Biological synthesis of nanoparticles: An environmentally benign approach, in Fundamentals of Nanoparticles. 2018, Elsevier. p. 571-604. https://doi.org/10.1016/B978-0-323-51255-8.00023-9  
  14. Wibowo, A., et al., Green Synthesis of Silver Nanoparticles Using Extract of Cilembu Sweet Potatoes (Ipomoea batatas L var. Rancing) as Potential Filler for 3D Printed Electroactive and Anti-Infection Scaffolds. Molecules, 2021. 26(7): p. 2042. https://doi.org/10.3390/molecules26072042  
  15. Al-Sarray, A.J., et al., Organo-Clay Composites of Intercalated 4-Methylaniline and Its Schiff Base Derivative: Preparation and Characterization. Journal of Medicinal and Chemical Sciences, 2022. 5(6): p. 1094-1101.  
  16. Said, M.M., et al., Multifunctional hydroxyapatite/silver nanoparticles/cotton gauze for antimicrobial and biomedical applications. Nanomaterials, 2021. 11(2): p. 429. https://doi.org/10.3390/nano11020429  
  17. Sharma, S.N. and R. Srivastava, Silver oxide nanoparticles synthesized by green method from Artocarpus Hetrophyllus for antibacterial and antimicrobial applications. Materials Today: Proceedings, 2020. 28: p. 332-336. https://doi.org/10.1016/j.matpr.2020.02.233  
  18. Al-Sarray, A.J.A., et al., Dielectric and Electrical Properties of Intercalated 1-(4-nitrophenyl)-N-(p-tolyl) methanimine into the Interlayers of Bentonite Clay. Journal of Medicinal and Chemical Sciences, 2022. 5(7 (Special Issue)): p. 1321-1330.  
  19. Rolim, W.R., et al., Green tea extract mediated biogenic synthesis of silver nanoparticles: Characterization, cytotoxicity evaluation and antibacterial activity. Applied Surface Science, 2019. 463: p. 66-74. https://doi.org/10.1016/j.apsusc.2018.08.203  
  20. Zhang, W. and W. Jiang, Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method. International Journal of Biological Macromolecules, 2020. 155: p. 1252-1261. https://doi.org/10.1016/j.ijbiomac.2019.11.093  
  21. Selvan, D.A., et al., Garlic, green tea and turmeric extracts-mediated green synthesis of silver nanoparticles: Phytochemical, antioxidant and in vitro cytotoxicity studies. Journal of Photochemistry and Photobiology B: Biology, 2018. 180: p. 243-252. https://doi.org/10.1016/j.jphotobiol.2018.02.014  
  22. Sweet, M.J. and I. Singleton, Soil contamination with silver nanoparticles reduces Bishop pine growth and ectomycorrhizal diversity on pine roots. Journal of Nanoparticle Research, 2015. 17(11): p. 1-8. https://doi.org/10.1007/s11051-015-3246-4  
  23. Ramachandraiah, K., N.T.B. Gnoc, and K.B. Chin, Biosynthesis of silver nanoparticles from persimmon byproducts and incorporation in biodegradable sodium alginate thin film. Journal of food science, 2017. 82(10): p. 2329-2336. https://doi.org/10.1111/1750-3841.13865  
  24. Wang, F., et al., Extract of Ginkgo biloba leaves mediated biosynthesis of catalytically active and recyclable silver nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2019. 563: p. 31-36. https://doi.org/10.1016/j.colsurfa.2018.11.054  
  25. Chung, I.-M., et al., Inhibition of mild steel corrosion using Magnolia kobus extract in sulphuric acid medium. Materials Today Communications, 2020. 25: p. 101687. https://doi.org/10.1016/j.mtcomm.2020.101687  
  26. Shende, S., et al., Platanus orientalis leaf mediated rapid synthesis of catalytic gold and silver nanoparticles. J Nanomed Nanotechnol, 2018. 9(494): p. 2. https://doi.org/10.4172/2157-7439.1000494  
  27. Haq, S., et al., Green synthesis of silver oxide nanostructures and investigation of their synergistic effect with moxifloxacin against selected microorganisms. Journal of Inorganic and Organometallic Polymers and Materials, 2021. 31(3): p. 1134-1142. https://doi.org/10.1007/s10904-020-01763-8  
  28. El-Ghmari, B., H. Farah, and A. Ech-Chahad, A new approach for the green biosynthesis of Silver Oxide nanoparticles Ag2O, characterization and catalytic application. Bulletin of Chemical Reaction Engineering & Catalysis, 2021. 16(3): p. 651-660. https://doi.org/10.9767/bcrec.16.3.11577.651-660  
  29. Alaallah, N.J., et al., Eco-friendly approach for silver nanoparticles synthesis from lemon extract and their anti-oxidant, anti-bacterial, and anti-cancer activities. 2023. 10(1): p. 205-216. https://doi.org/10.18596/jotcsa.1159851  
  30. Hemath Naveen, K., et al., Extracellular biosynthesis of silver nanoparticles using the filamentous fungus Penicillium sp. Arch. Appl. Sci. Res, 2010. 2(6): p. 161-167.  
  31. Stoehr, L.C., et al., Shape matters: effects of silver nanospheres and wires on human alveolar epithelial cells. Particle and fibre toxicology, 2011. 8(1): p. 1-15. https://doi.org/10.1186/1743-8977-8-36  
  32. Elemike, E.E., et al., Green synthesis of Ag/Ag2O nanoparticles using aqueous leaf extract of Eupatorium odoratum and its antimicrobial and mosquito larvicidal activities. Molecules, 2017. 22(5): p. 674. https://doi.org/10.3390/molecules22050674  
  33. Al-Noor, T.H., et al., A Short Review: Chemistry of Curcumin and Its Metal Complex Derivatives. Journal of University of Anbar for Pure Science, 2022. 16(1): p. 20-26. https://doi.org/10.37652/juaps.2022.174832  
  34. Li, R., et al., Biosynthesis of silver oxide nanoparticles and their photocatalytic and antimicrobial activity evaluation for wound healing applications in nursing care. Journal of Photochemistry and Photobiology B: Biology, 2019. 199: p. 111593. https://doi.org/10.1016/j.jphotobiol.2019.111593  
  35. Rashmi, B., et al., Facile green synthesis of silver oxide nanoparticles and their electrochemical, photocatalytic and biological studies. Inorganic Chemistry Communications, 2020. 111: p. 107580. https://doi.org/10.1016/j.inoche.2019.107580  
  36. Verkhovskii, R., et al., Physical properties and cytotoxicity of silver nanoparticles under different polymeric stabilizers. Heliyon, 2019. 5(3): p. e01305. https://doi.org/10.1016/j.heliyon.2019.e01305  
  37. Gurunathan, S., et al., Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy. International journal of nanomedicine, 2015. 10: p. 4203. https://doi.org/10.2147/IJN.S83953  
  38. Al-Sarray, A.J.A., Molecular and electronic properties of Schiff bases derived from different aniline derivatives: density functional theory study. Eurasian Chemical Communications, 2023. 5(4): p. 317-326.  
  39. Kovács, D., et al., Silver nanoparticles defeat p53-positive and p53-negative osteosarcoma cells by triggering mitochondrial stress and apoptosis. Sci Rep, 2016. 6: p. 27902. https://doi.org/10.1038/srep27902  
  40. Gao, M., et al., Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells. Biomaterials, 2017. 130: p. 14-27. https://doi.org/10.1016/j.biomaterials.2017.03.027
Nanomedicine Research Journal
Volume 8, Issue 3
July 2023
Pages 259-267

Files

Share

How to cite

Statistics