Mahnaz Kesmati; Mozhgan Torabi; Nahid Pourreza; Mehrnaz Tayebkhah; Fereshteh Asadi
Abstract
Objective(s): Based on our previous studies about nano-ZnO effects on physiological behaviors, in this study we have investigated the effects of anxiolytic doses of nano-ZnO on electrocardiogram (ECG) sings changes in the restraint and non-restraint ovariectomized (OVX) female rats. Methods: Animals ...
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Objective(s): Based on our previous studies about nano-ZnO effects on physiological behaviors, in this study we have investigated the effects of anxiolytic doses of nano-ZnO on electrocardiogram (ECG) sings changes in the restraint and non-restraint ovariectomized (OVX) female rats. Methods: Animals (160-180 g) were divided into: control (non-OVX + saline) and OVX groups that received: saline, nano-ZnO 1, 2.5 and 5 mg/kg, stress of 90 or 180 min + saline and stress of 180 min+ nano-ZnO 2.5 mg/kg. Anxiety-like behaviors were measured by the elevated plus maze (EPM) and hole board apparatus thirty min after intraperitoneally injections or stress induction. ECG parameters and serum zinc level were measured in all groups. Results: Ovariectomy induced anxiety and restraint stress in OVX rats increased it in the hole board test. Nano-ZnO 2.5 and 5 mg/kg improved anxiety-like behaviors in the EPM test. Nano-ZnO 2.5 mg/kg improved anxiety induced by the restraint stress in the hole board test. Nano-ZnO increased zinc level in a dose-dependent manner in the non-restraint OVX groups. Nano-ZnO 1 mg/kg decreased QRS amplitude and all doses decreased QT interval to the level of non-OVX group. Nano-ZnO 5 mg/kg decreased QTc to the level of non-OVX group. Stress of 90 min increased QT interval while stress of 180 min decreased it. Nano-ZnO after stress induction could alleviate heart rate, R-R- interval and QRS interval to the level of non-OVX group. Conclusion: It seems that nano-ZnO rather than could improve anxiety, alleviated ECG parameters in the restraint and non-restraint OVX rats.
Mozhgan Torabi; Mahnaz Kesmati; Nahid Pourreza; Hossein Najafzadeh Varzi; Hamid Galehdari
Abstract
Objective(s): Stress is a physiological response that can disrupt body elements homeostasis and lead to neurophysiological abnormality. This study has been investigated the serum and hippocampus Mg2+, Zn2+, Fe2+/3+ and Ca2+ level changes in two times after MgO NPs and ZnO NPs single injection following ...
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Objective(s): Stress is a physiological response that can disrupt body elements homeostasis and lead to neurophysiological abnormality. This study has been investigated the serum and hippocampus Mg2+, Zn2+, Fe2+/3+ and Ca2+ level changes in two times after MgO NPs and ZnO NPs single injection following restraint stress in the male rat. Methods: Animals were divided into two main groups that each of them includes: control, restraint of 90, 180 and 360 min+ saline, MgO NPs and ZnO NPs 5 mg/kg alone and with a restraint of 90 min. In one group, 30 min and in another 120 min after intraperitoneally injections of components or stress induction elements levels were measured in the serum and hippocampus. Results: MgO NPs and ZnO NPs could change elements level in the serum and hippocampus depend on acute time after injections and there was a significant positive correlation between serums Fe2+/3+ following two different acute times after ZnO NPs administration. Different times of stress induction have different effects on elements level changes in the serum and hippocampus, 30 and 120 min after induction and nanoparticles could alleviate these changes depend on the time. In restraint groups, there were positive and negative significant correlations between two different times measurements of Fe2+/3+ or Ca2+ in the serum and hippocampus. Conclusion: it seems that time is an important factor in ameliorative MgO NPs and ZnO NPs effects on elements disruption induced by stress, but their exact interaction with stress systems containing ions level changes needs to more investigation.
