Document Type : Original Research Article
1 Nanotechnology Research Center, Azad University- Tehran South Branch, Tehran, Iran. and Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2 Nanotechnology Research Center, Azad University- Tehran South Branch, Tehran, Iran. and Department of Biomedical Engineering, Islamic Azad University, South Tehran Branch, Tehran Iran
3 Nanotechnology Research Center, Azad University- Tehran South Branch, Tehran, Iran. and Department of Chemistry, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
4 Nanotechnology Research Center, Azad University- Tehran South Branch, Tehran, Iran.
5 Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
The treatment of bacterial infections has been dramatically compromised by the persistent emergence of gram-positive and gram-negative antibiotic resistant strains (1,2). The Gram-negative bacterium Pseudomonas aeruginosa is an opportunistic pathogen that normally inhabits the soil and surfaces in aqueous environments such as water and soil ecosystems and infective to plants, animals and humans (3-5). Pseudomonas aeruginosa is considered the most medically important among the genus Pseudomonas that frequently causes hospital-acquired and opportunistically infections for example pneumonia, urinary tract infections and wound infections (6,7). Infections caused by P. aeruginosa are often severe and life threatening and are difficult to treat because of the limited susceptibility to antimicrobial agents. (8). Pseudomonas aeruginosa is a notoriously difficult organism to control with antibiotics or disinfectants. (9). On the other hand, Nanoantibiotics have recently developed as a hopeful technology to improve outcomes of infections. moreover, attention has been focused on novel attractive nano-based materials with antibacterial activity. (10,11). In the last few years, one-dimensional nanomaterials such as nanofibers, nanowires and nanorods have been attracting a great interest of researchers due to their unique optical, thermal, electrical, magnetic, and mechanical properties and they have been used in many applications such as catalysis, energy storage, nanoreactors, sensors, drug delivery and biomedical engineering [12,13]. The main advantage of one-dimensional structures is their high surface area, which provides many special properties and functionalities .
Electrospinning technique is a simple, convenient, low cost and versatile method which has been used to produce one-dimensional nanostructure materials . Most soluble polymers with appropriate molecular weight can be electrospun. Polymeric nanofibers have been used in many applications including filters, reinforcing agents, biomedical materials, tissue engineering, catalysis, sensing, fabrication of composite materials, supercapacitors, fiber templates to prepare nanotubes and wound dressing [16-18]. Also, this technique has the ability to produce a three-dimensional nanofibrous membrane with a large surface area .
Poly (diallyldimethylammonium chloride) (PDDA) is a linear cationic polyelectrolyte which is widely used in industrial applications such as flocculants, dewatering agents, emulsifiers, papermaking, mining and petrol industry . Also, it is used as a model for studies in different areas of polyelectrolyte research and functionalizing nanomaterials . The functionalized graphene nanosheets with PDDA were synthesized and used to combine with room temperature ionic liquid (RTIL) . Formation of unique micro- and nanostructures from the combination of the anionic surfactant sodium dodecyl sulfate (SDS) with cationic PDDA polyelectrolyte was evaluated . In another research, PDDA and Sodium Silicate Multilayers are coated on silica-sphere to prepare a superhydrophobic surface .
On the other hand, the polyelectrolyte PDDA has been considered as a safe compound for human health. Also, it has a biocompatible, biodegradable and nontoxic nature. This homopolymer bears permanently charged quaternary ammonium groups in its cyclic unities which enable it to be classified as antimicrobial agents . Cationic bilayer fragment/ carboxymethylcellulose (CMC)/ PDDA nanoparticle with high antibacterial property is synthesized by researchers . Also, the antimicrobial activity of PDDA/ poly (methylmethacrylate) nanoparticles is investigated . The cationic bilayer fragments/amphotericin B/carboxymethyl cellulose/ PDDA nanoparticles with the releasing ability of amphotericin B are prepared .
Moreover, Honey is a natural high nutritional and prophylactic viscous food which is generally composed of water, glucose, sucrose, fructose, and many other substances. Honey is shown medicinal properties and it has been used in wound care due to its antimicrobial property . Also, the fabrication of the electrospun honey-based fibrous matrices could be possible. In this regard, the poly (vinyl alcohol) (PVA)/honey electrospun fibers with a uniform and bead-free morphology are prepared . Also, the polyethylene terephthalate (PET)/ honey/chitosan nanofibers aiming to produce a wound dressing are produced . However, a literature review showed that there is no study on preparation of PDDA or PDDA/honey nanofibers. In this research, the ability of the PDDA nanofibers producing with two molecular weights and antibacterial activity of novel antibiotic free antibacterial PDDA/honey nanofiber was investigated. The amount of honey in the spinning solution and the electrospinning parameters were optimized aiming to produce a uniform and bead-free morphology. Finally, the obtained nanofibers were crosslinked by glutaraldehyde to decrease the solubility and improve the water absorption property. Antibacterial results showed that the PDDA/honey nanofibers have been high antibacterial activity against gram-positive and gram-negative bacteria. Moreover, investigation of antibacterial activity of antibiotic free nanofiber against Pseudomonas aeruginosa (P. aeruginosa) demonstrated the novel PDDA/honey nanofiber is suitable agent for the treatment of pathogenic P. aeruginosa infection.
Poly (diallyldimethylammonium chloride) (PDDA) with the medium molecular weight (Mw=200,000-350,000), 20 wt% in H2O and high molecular weight (Mw=450,000), 40 wt% in H2O were purchased from Sigma Aldrich (Germany). Raw Persian Honey in name of Thyme was purchased from a local producer (Polour, Iran). Glutaraldehyde (GA) (50% in water) was as received from Merck (Germany).
Preparation of nanofibers
The PDDA solution was used as received without any change in its concentration. The produce ability of PDDA nanofibers was investigated at different conditions including wide ranges of high voltage (14-23 kV) and feeding rate (0.35-1mL/h). In order to produce the PDDA/honey nanofibers, different spinning solutions was prepared by adding the various amount of honey (30-70%w/w, respect to the PDDA) to the PDDA solution. Then, 1 mL ethanol was added to the solutions (the volume of mixed solution was 5 mL) under stirring at room temperature for 1h. The prepared solutions were then electrospun under a wide range of high voltage (14-23 kV) when the distance between tip of needle and collector was 16cm. The electrospinning apparatus was from Fanavaran nano-meghyas Co. (Iran) and nanofibers were collected onto aluminum (Al) sheet. The feeding rate of the polymer solution was changed in range of 0.35-1 mL/h.
Crosslinking of nanofibers
The nanofiber mats (15 cm×١٥ cm) were attached to polyethylene frames and placed into a sealed chamber saturated with the vapor of 40 mL of GA solution. The nanofibers were exposed to GA vapor for different times (2, 3, 4 and 5) and then placed in a vacuum oven at 70 °C for 12 h to remove residual glutaraldehyde.
The surface morphology of nanofibers was characterized by Scanning Electron Microscope (SEM, LEO1455VP, ENGLAND). The FTIR spectra of nanofibers were examined by the FTIR spectroscopy (ThermoNicolet NEXUS 870 FTIR from Nicolet Instrument Corp., USA).
The antibacterial activity of nanofibers against Escherichia coli (ATCC 25922) a Gram-negative bacteria and S. aureus (ATCC 25923) a Gram-positive bacterium was measured by using the viable cell counting method. 100 µL E. coli and S. aureus were cultivated in 100 mL of a nutrient broth solution individually. Then, 1 mL of the bacteria/nutrient solution was added to 9 mL of sterilized nutrient broth solution (0.8%). Bacteria concentration of 5×١٠5 CFU/mL was selected for the test. The weight and size of samples were 100 mg as disks of 2.8 cm diameter. The antibacterial test was performed by putting the nanofibers into 10 mL of the bacteria/nutrient solution. After the exposure of the bacteria to the nanofibers, the bacterial solution (100 µL) was taken out and quickly spread on a plate containing nutrient agar. Plates containing bacteria were incubated at 37 °C for 24 h, and then the numbers of the surviving colonies were counted.
Water absorbency measurement was performed via gravimetric analysis. 0.10 g of nanofibers was immersed in 50 mL of distilled water at room temperature (25 °C) for 120 min and pH=7.4. Then, the nanofibers were separated and weighed. The water absorbency (Q; g.g−1) was calculated as follows.
where mb (g) and ma (g) are the weights of the samples in the dry state and the swollen state at a certain time, respectively .
The solubility of nanofibers was investigated by immersing the nanofibers in a batch containing distilled water for 120 min at 25°C and pH=7.4. After that, the nanofibers were separated and dried at 60°C for 6h. They were weighed and the solubility was calculated using the following equation.
where mx and my are the weight of nanofibers before and after the immersing in distilled water, respectively.
RESULTS AND DISCUSSION
In order to control the fiber characteristics, the electrospinning parameters can be adjusted. Viscosity, molecular weight of the polymer, applied voltage, charge density and distance from needle to collector are of important parameters affecting directly on the nanofiber morphology .
In this study two molecular weights of PDDA polymer were used to produce nanofibers. The nanofibers could not be obtained with lower molecular weight of PDDA polymer. Fig. 1 showed the SEM images of PDDA nanofibers prepared by the polymer with high molecular weight at different magnifications. A wide range of high voltage (14-23 kV) was applied to the polymer droplet to produce fibrous structure. As can be seen, many defects such as bead and droplets existed in the nanofiber morphology. Also, there were a lot of unspun droplets during the process which was resulted from the influence of the gravitational force . However, the reproducibility of spinning procedure was not feasible and the electrospinning conditions including flow rate and voltage were varied for each test. Reproducibility and stability are the important factors of the electrospinning process . The formation of droplets among the nanofibers can be due to the capillary breakup of the spinning jet by surface tension. In this case, the distance of polymer chains in the solvent were relatively far and the effective entanglement could not be occurred.
In order to enhance the spinning conditions and obtain a uniform morphology, honey with different concentrations (30, 40, 50, 60 and 70%w/w respect to PDDA concentration) was added to the PDDA solution. Nanofibers with the concentration ratio of 70/30, 60/40 and 30/70%w/w (PDDA/honey) were not obtained. At low concentration of honey (30 and 40%w/w), the viscosity of solution was low and a long continuous liquid jet was not formed. On the other hand, raising the honey concentration to 70%w/w caused to a significant increase in the viscosity value of spinning solution. Higher viscosity values result to higher entanglement of the polymer chains . Fig. 2 showed the changes of the viscosity as a function of the shear rate for various solutions at 25°C. It was clear that the shear thinning effect occurred for the PDDA solution (the viscosity decreases with raising the shear rate). In this case, at low shear rates, the polymer chains are not aligned with the direction of flow. This caused to appearance of a relatively high viscosity value. With increasing the shear rates, the number of aligned polymeric chains to the direction of flow increased resulted to a lower viscosity value.
Honey with different origins showed various rheological behaviors. Most of the honeys are reported to have Newtonian fluid like characteristics, whereas, some honey showed non-Newtonian fluids properties . Some others also exhibited a thixotropic behavior . The thyme Persian honey, which was used in this study, showed a Newtonian fluid like characteristics. The apparent viscosity remained nearly constant with increasing shear rates. Such a result is reported by other researchers . However, the PDDA solution with concentration ratio of 30/70, 40/60 and 50/50 showed a Newtonian fluid like behavior which can be due to the high concentrations of honey in the mixed solution. With increasing the PDDA concentration the changes in the viscosity values increased. For the solutions with the PDDA concentration of 60 and 70%w/w, the viscosity decreased with raising the shear rate (shear thinning effect). The viscosity value for the PDDA, honey, and the 70/30, 60/40, 50/50, 40/60, 30/70 PDDA/honey solutions at the shear rate of 100 was 0.791, 22.1, 2.28, 4.17, 5.91, 7.01 and 9.18 Pa.s., respectively. It was clear that solution viscosity increased with raising the honey concentration.
Fig. 3 showed the SEM images of PDDA/honey nanofibers with the concentration ratio of 50/50%w/w at different applied voltages and the flow rate of 0.8 mL/h. From the figure, all the nanofibers presented a beaded structure. Also, the diameter of nanofibers was not uniform. The increase in the high voltage values (18-20 kV) caused to increase the charge density in the liquid jet and polymer jet velocity. An increment in the velocity of jet increased the applied elongation forces resulted to more uniform morphology of nanofibers and decreasing the amount of beads. However, the shape of droplet on the tip of needle was not uniform during the spinning process. Also, receded jets, which were not stable jets, were replaced by cone jets and caused to increase the un-uniformity .
At low high voltage values (14-17 kV), the applied electric field was not strong enough to provide a balance between the electrostatic repulsion, the surface tension and viscoelastic force resulted to formation of a beaded structure. In the electrospinning process, providing a balance between the surface tension, electrostatic repulsion, and viscoelastic forces results a uniform morphology of nanofibers . In this study, the effects of viscoelasticity and charge density on fiber morphology were evaluated by varying the concentration ratio of polymer and voltage, respectively. With increasing the high voltage values (18-20 kV), the fibrous structure was obvious and the amount of bead was decreased. However, the dividing of polymer droplet on the tip of needle to small droplets was occurred which can be due to the low viscosity of the spinning solution. An investigation on the average nanofiber diameter revealed that increasing the voltage value from 18 to 19 kV caused to decrease the nanofiber diameter. However, the diameter was increased when the voltage rose to 20 kV (Fig. 3C). Increasing the nanofiber diameter with increasing the high voltage value is reported by researchers . Further increase in the voltage value (22 kV) resulted to raising the amount of bead probably due to the unbalance conditions of electrospinning process.
The effect of flow rate on nanofiber morphology was also investigated at the voltage of 19 kV and the result is shown in the Fig. 4. It was found that the beaded structure was obtained with the flow rate of 0.6 mL/h. Increasing the flow rate to 0.8 mL/h resulted to more uniform morphology with lower amount of bead compared to 0.7 mL/h probably because of the existence of a balance between the amounts of injection and feeding of polymer in the spinning process.
Fig. 5 presented the SEM images of nanofibers produced with the honey concentration of 60%w/w with the flow rate of 0.8 mL/h. It was clear that increasing the honey concentration to 60%w/w was enhanced significantly the uniformity of the produced nanofibers. An increment in the voltage value from 14.5 to 16.5 kV caused to decrease the nanofiber diameter due to the stretching of the polymer droplet along with charge repulsion within the polymer jet . Raising the voltage value (14.5 to 16.5 kV) resulted in enhancing the uniformity of nanofiber diameter because of the stability of the jet due to increased charge density on the surface of jet, the jet velocity and the applied elongation forces . The average diameter of nanofibers obtained at the voltage of 16.5 kV was 94.1nm. The average diameter of PDDA/honey nanofibers was nearly constant in the voltage range of 17-19 kV. Previously, it is reported that for certain polymers, increasing the applied voltage did not effect on the diameter and morphology of nanofibers . The nanofibers with smooth surface, uniform morphology and the average diameter of 91.8 nm were obtained at the high voltage of 19 kV. Further increase in the voltage value changed the morphology of nanofibers from round to ribbon-like shape. Increasing the high voltage caused to apply high electrostatic and elongation forces to the droplet and liquid jet. Hence, the polymeric jet moves faster toward the collector and the solvent may not dry completely before reaching the collector. These reasons led to the formation of ribbon-like structure. Moreover, the width of nanofibers increased with raising the voltage values.
Fig. 6 showed the distribution of nanofiber diameter with the high voltage range of 14.5-16.5 and 17-19 kV. It was clear that the nanofibers produced with the voltage of 14.5 kV presented a relatively wide distribution with the center of 124 nm compared to the other samples. The diameter distribution became narrow when the applied voltage rose to 16.5 kV. The average diameter of nanofibers produced at the high voltage of 15.5 and 16.5 kV was 108.4 and 94.1 nm, respectively. This can be probably due to the more stable condition of electrospinning process resulted to produce bead-free nanofibers with more uniform diameter. The diameter distribution of nanofibers obtained at the high voltage range of 17-19 kV exhibited a narrow structure with the center of 93.5, 92.7 and 91.8 nm for the voltage of 17, 18 and 19 kV, respectively.
The effect of flow rate on the nanofiber morphology was investigated for the PDDA/honey nanofibers with the honey concentration of 60%w/w at the high voltage of 19 kV and the result is shown in Fig. 7. It was found that the nanofibers were not obtained when the flow rate was lower than 0.35 mL/h. This can be due to insufficient amount of solution in the spinneret. In this case, the Taylor cone at the tip of needle could not be formed. With increasing the flow rate to 0.6 mL/h, uniform nanofibers with round cross section were produced. According to the Fig. 7, the uniform morphology was maintained when the flow rate was increased from 0.6 to 0.8 mL/h. On the other hand, an increment in the flow rate from 0.6 to 0.8 mL/h increased the nanofiber diameter from 79 to 91.8 nm, respectively. Increasing the flow rate to 1 mL/h caused to form the ribbon-like structure because of incomplete evaporation of solvent resulted from high amount of injected polymer .
An increment in the content of honey caused to increase the spinning viscosity. At the low viscosity (PDDA/honey with the concentration ratio of 50/50%w/w), the mobility of the PDDA polymer chains was high and the strong instabilities of the jets occurred during the spinning process. These were resulted to formation of beads in the nanofiber morphology. Increasing the viscosity resulted from the increment of honey content in the spinning solution increased the entanglement of polymer chains. Hence, the liquid jet was more stable and fibrous morphology was obvious. Such a result is reported by researchers [42,44]. According to the results of the microscopic analysis, the PDDA/honey nanofibers with the concentration ratio of 40/60%w/w obtained at the high voltage of 17kV and the flow rate of 0.8mL/h were selected as the optimum and used for crosslinking procedure and further studies. The crosslinking of nanofibers was performed by glutaraldehyde. The SEM images of the crosslinked nanofibers with different crosslinking time are shown in Fig. 8. According to the images, some conglutinations of nanofibers especially at their touching points and some cracks on the nanofiber surface were observed. Increasing the time of crosslinking changed the morphology from fibrous to film like structure.
The FTIR spectra of PDDA, PDDA/honey (40/60%w/w) and crosslinked PDDA/honey samples are shown in Fig. 9. In the PDDA spectrum, the band at 3434 and 1631 cm-1 were attributed to the stretching and bending vibrations of absorbed water molecules, respectively. Also, the appeared band at 1205 cm-1 was related to the stretching vibration of C-N bond . The peak observed at 1460 cm−1is due to CH2 bending vibration. The bands at 2931 and 2898 cm–1 assigned to the methylene C-H asymmetric and symmetric stretching vibrations, respectively. The honey composed of mainly hydrated glucose, fructose and carbohydrates. The skeletal vibrations carbohydrates were appeared in the range of 600-1500 cm-1 . Also, the band at 3443 cm-1 was related to the stretching vibration of OH groups of carbohydrate. The appeared band at 2994 and 2841 cm-1 were assigned to the stretching vibrations of CH bonds. The band at 1513 cm-1 is attributed to the vibration of C=C bond in the aromatic ring  of polyphenols in the honey. Amino acids, existed in the honey, generally contain amide groups with several characteristic bands as amide A (about 3500 cm-1), amide B (about 3100 cm-1), amide I (1600–1700 cm-1), amide II (1550 cm-1) and amide III (1300–1350 cm-1) depending on the type and amount of amino acids. The characteristic peaks of PDDA overlapped with the honey characteristic peaks. By comparing the curves B and C, it was found that the intensity of bands at 1059, 2936, 2848 and 1731 cm-1 increased which were related to the acetal band , resulted from the reaction of glutaraldehyde with the hydroxyl group of honey, the stretching vibrations of CH bonds and the stretching vibration of C=O groups, existing in the glutaraldehyde , respectively. Also, the intensity of the band at 3446 was cm-1 decreased due to the reaction of crosslinking agent with OH groups of honey. A new absorption peak at 1690 cm-1 was related to the imine group , resulted from the reaction of glutaraldehyde and amine group of honey contents.
The wound moist environment is an ideal growth medium for bacteria. Infection has been identified as a major factor in impaired healing. Wound infections are largely treated with antibiotics in conventional medicine however, it is often inappropriate in cases such as burns and chronic wounds. In this regard, dressing technology is known as alternative options. Among the various materials used in wound dressing, honey containing dressing materials is highly desired due to the ability of control the level of wound hydration and its antimicrobial property .
The amount of water uptake of nanofibers was measured as 0.4, 4.9, 3.6 and 0.2 g/g for the crosslinked nanofibers with the crosslinking time of 2, 3, 4 and 5 h. Increasing the time of crosslinking changed the morphology of nanofibers. Also, the crosslinked nanofibers with the time of 4 and 5 h presented a rigid structure with no flexibility which is not favor for wound dressing. Increasing the time of crosslinking caused to change the hydrophilic nature of the nanofiber surface, hindering the penetration of water molecules in to the nanofiber mats. The result of nanofibers solubility revealed that the samples crosslinked with the time of 2 h showed a weight loss of 39.3% whereas there was not any weight loss for the other samples. In this regard, the crosslinking time of 3 h was selected as optimum.
The antibacterial activity of PDDA and PDDA/honey nanofiber (40/60%w/w) samples were studied by using the viable cell-counting method. The effects of nanofibers on the growth of the gram-positive and gram-negative bacteria (S. aureus and E. coli), also antibiotic resistant bacteria (P. aeruginosa) were investigated and the results are shown in Fig. 10 and 11. Fig. 10 as shown in the plates, the number of bacteria colonies decreased after the addition of honey to the nanofibers. For the PDDA/honey nanofiber sample, of E. coli and S. aureus with initial number concentration of 5×١٠5 CFU/mL were completely eradicated whereas there were some bacterial colonies for the PDDA sample. Also, the antibacterial efficiency of PDDA sample for E. coli was slightly higher than S. aureus. The antibacterial efficiency of PDDA sample was 77.8 and 81.2% for S. aureus and E. coli, respectively. Additionally, PDDA/honey nanofiber (40/60%w/w) antibacterial activity against P. aeruginosa was studied using same study condition of S. aureus and E. coli, results indicated antibacterial efficiency of antibiotic free PDDA/honey nanofiber was suitable. PDDA/honey nanofiber (40/60%w/w) determined 98.89% antibacterial efficiency for P. aeruginosa as important medically the genus Pseudomonas. The antibacterial activity of PDDA as a polycation polymer is related to permanently charge quaternary ammonium groups in its cyclic unities which interact with the negatively charged surfaces of bacteria, resulting in loss of membrane permeability, cell leakage, and finally cell death . The antibacterial property of honey was related to the H2O2 and antioxidant polyphenols existing among its compounds. Polyphenols in the presence of transition metal ions play a powerful pro-oxidants role, stimulating the generation of hydroxyl radicals from H2O2 via the Fenton reaction . The antibacterial efficiency of PDDA/honey antibiotic free nanofiber was 99.9 % for S. aureus and E. coli. The results showed synergistic antibacterial effect of the PDDA/honey blend composite, compared to honey or PDDA only. Moreover, antibacterial PDDA/honey nanofiber dilution did not show a significant effect on the antibacterial response (Fig. 11).
In this research, the ability of producing the PDDA nanofibers was investigated for first time. The result showed that the PDDA nanofibers with the beaded morphology can be obtained with high molecular weight of polymer and producing the PDDA nanofiber with low molecular weight of polymer is impossible. However, the reproducibility of nanofibers was not feasible at the same condition. In order to obtain a uniform morphology, different concentrations of honey were added to the spinning solution. The addition of honey increased the solution viscosity and entanglement of polymer chains. The bead-free nanofibers with uniform diameter were obtained with concentration ratio of 40/60 (PDDA/honey). Increasing the high voltage values caused to decrease the nanofibers average diameter. However, the average diameter of nanofibers was nearly constant when the high voltage ranged from 17 to 19 kV. Increasing the flow rate of spinning solution increased the average diameter of nanofibers. An increment of flow rate to 1 mL/h and voltage value to 21 kV resulted in formation of ribbon-like structure due to incomplete evaporation of solvent and high applied electrostatic and elongation forces to the droplet and liquid jet. The use of the same concentration of PDDA and honey resulted to formation of beaded structure. The density of beads was reduced with varying the electrospinning parameters (high voltage and flow rate values). However, nanofibers with uniform diameter and bead-free morphology were not obtained due to the low viscosity of spinning solution. Crosslinked nanofibers showed a high water absorption capacity and antibacterial property. Moreover, PDDA/honey antibiotic free nanofibers showed great antibacterial activity (99.9 %) against S. aureus and E. coli and suitable activity (98.9) against pathogenic bacteria (P. aeruginosa). Finally, PDDA/honey antibiotic free nanofibers with suitable antibacterial activity can be used as a nanoantibiotic against gram-positive and gram-negative bacteria, such as pathogenic P. aeruginosa as medically the genus Pseudomonas. As well as, it can be suggested that the prepared novel antibiotic free nanofibers can be a good candidate for wound dressing.
CONFLICT OF INTEREST
The authors declare that there are no conflicts of interest.