Nanomedicine Research Journal

Nanomedicine Research Journal

Compression of radio and photo sensitivity of 5-aminolevulinic acid (5-ALA) conjugated hollow gold nanoparticles (HGNs) on KYSE cell line of oesophageal cancer

Document Type : Original Research Article

Authors
Zahra Mohammadi 1
Armin imanparast 2
Hoda Talebian 3
Nafiseh Sobhani 4
Maryam Shabanzadeh 5
Ameneh Sazgarnia 2
1 Radiological Technology Department of Faculty Paramedical Sciences, Babol University of Medical Sciences, Babol, Iran
2 Medical Physics Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Medical Physics Radiobiology and Radiation Protection, School of Medicine, Babol University of Medical Sciences, Babol, Iran
4 PhD Candidate of Neuroimaging at the Tehran University of Medical Sciences, Tehran, Iran
5 Islamic Azad University Science and Research Branch, Tehran, Iran
10.22034/nmrj.2024.03.007
Abstract
Objective(s): Nanoparticles can be used in therapies by increasing drug delivery and radiosensitivity. In this study, we investigated the radio- and photo-sensitivity of the conjugation of 5-aminolevulinic acid (5-ALA) and hollow gold nanoparticles (conjugate) on the KYSE cells. 
Methods: After the determination of the dark toxicity of the therapeutic agent, the experiments were performed at three different radiation doses. Then, the photodynamic effect of each of group was analyzed at three optical doses.
Results: There was no significant difference between the control and treatment groups in X-ray treatment. In the photodynamic therapy (PDT), a significant difference was noted between the control group and the treatment groups, particularly within the 5-ALA and conjugate groups. Notably, the conjugate group induced approximately 90% cell death.
Conclusions: The study found that the conjugate induced a cell death rate more than three times higher than that of free 5-ALA alone. Thus, it can be concluded that the conjugate serves as an effective delivery agent for 5-ALA and enhances tumor cell destruction following PDT.
Keywords
Hollow gold nanoparticles (HGNs)
5-aminolevulinic acid
Radiotherapy
PDT
oesophageal cancer

Subjects

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Nanomedicine Research Journal
Volume 9, Issue 3
Autumn 2024
Pages 298-307