Nanomedicine Research Journal

Nanomedicine Research Journal

Magnetic loaded compritol ATO based lipid carriers as a targeted anti-cancer drug delivery system

Document Type : Original Research Article

Author
Elham Rostami
Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
10.22034/nmrj.2024.03.004
Abstract
In this study, we prepared Epirubicin-loaded magnetic solid lipid nanoparticles for intravenous drug dosing. Magnetic lipid carriers were fabricated using a hot microemulsion approach using stearic acid and Compritol ATO 888 as a core of particles. Prepared nanoparticles are characterized using transtition electon microscopy, photon correlation spectroscopy, Fourier transforms infrared spectroscopy, and vibrating sample magnetometer. The size of nanoparticles was about 130 nm after drug loading. Also, entrapment efficiency, drug loading, in vitro drug release, and release kinetic were investigated in detail. In vitro cell cytotoxicity and biocompatibility of particles evaluated by MCF-7 cell lines. The entrapment efficiency of 86±4.5% and 51.7±3.5% were obtained for solid lipid and magnetic solid lipid nanoparticles respectively. Size investigation showed that prepared NPs have an increase in particle size with magnetic loading. In vitro cytotoxicity of the formulations on the MCF-7 cell line demonstrated the greater toxicity of drug-loaded nanoparticles compared to the free drug. This study proved the efficiency of lipid-based carriers in drug dosing and targeting.  These studies showed that the Magnetic Lipid Nanoparticle (mSLN) has a very remarkable anticancer effect on the MCF-7 cell line in comparison with pure drug. 
Keywords
Magnetic Lipid Nanoparticle
Target drug delivery
Stearic Acid
Compritol ATO 888

Subjects

1.    Gottesman, M.M., O. Lavi, M.D. Hall and J.-P. Gillet, Toward a better understanding of the complexity of cancer drug resistance. Annual review of pharmacology and toxicology, 2016. 56: p. 85-102. https://doi.org/10.1146/annurev-pharmtox-010715-103111
2.    Wong, H.L., R. Bendayan, A.M. Rauth, Y. Li and X.Y. Wu, Chemotherapy with anticancer drugs encapsulated in solid lipid nanoparticles. Advanced drug delivery reviews, 2007. 59(6): p. 491-504. https://doi.org/10.1016/j.addr.2007.04.008
3.    Ying, X.-Y., D. Cui, L. Yu and Y.-Z. Du, Solid lipid nanoparticles modified with chitosan oligosaccharides for the controlled release of doxorubicin. Carbohydrate Polymers, 2011. 84(4): p. 1357-1364. https://doi.org/10.1016/j.carbpol.2011.01.037
4.    Rostami, E., Recent achievements in sodium alginatebased nanoparticles for targeted drug delivery, Polymer Bulletin, 2022. 79(2): p. 6885-6904. https://doi.org/10.1007/s00289-021-03781-z
5.    Rostami, E., S. Kashanian and A. Azandaryani, Preparation of solid lipid nanoparticles as drug carriers for levothyroxine sodium with in vitro drug delivery kinetic characterization. Molecular biology reports, 2014. 41(5): p. 3521-3527. https://doi.org/10.1007/s11033-014-3216-4
6.    Gasco, M.R., A. Mauro and G.P. Zara, 13 In Vivo Evaluations of Solid Lipid Nanoparticles and Microemulsions. Drug Delivery Nanoparticles Formulation and Characterization, 2016. 191: p. 219.
7.    Martins, S.M., B. Sarmento, C. Nunes, M. Lúcio, S. Reis and D.C. Ferreira, Brain targeting effect of camptothecin-loaded solid lipid nanoparticles in rat after intravenous administration. European Journal of Pharmaceutics and Biopharmaceutics, 2013. 85(3): p. 488-502. https://doi.org/10.1016/j.ejpb.2013.08.011
8.    Rostami, E. Progresses in targeted drug delivery systems using chitosan nanoparticles in cancer therapy: A mini-review, Journal of Drug Delivery Science and Technology, 2020 (58): p.101813. https://doi.org/10.1016/j.jddst.2020.101813
9.    Yang, S.C., L.F. Lu, Y. Cai, J.B. Zhu, B.W. Liang and C.Z. Yang, Body distribution in mice of intravenously injected camptothecin solid lipid nanoparticles and targeting effect on brain. Journal of controlled release, 1999. 59(3): p. 299-307. https://doi.org/10.1016/S0168-3659(99)00007-3
10.    Arduino, I., N. Depalo, F. Re, R. Dal Magro, A. Panniello, N. Margiotta, E. Fanizza, A. Lopalco, V. Laquintana and A. Cutrignelli, PEGylated solid lipid nanoparticles for brain delivery of lipophilic kiteplatin Pt (IV) prodrugs: An in vitro study. International journal of pharmaceutics, 2020. 583: p. 119351. https://doi.org/10.1016/j.ijpharm.2020.119351
11.    Zhu, L., Z. Zhou, H. Mao and L. Yang, Magnetic nanoparticles for precision oncology: theranostic magnetic iron oxide nanoparticles for image-guided and targeted cancer therapy. Nanomedicine, 2016(0). https://doi.org/10.2217/nnm-2016-0316
12.    Berry, C.C., Progress in functionalization of magnetic nanoparticles for applications in biomedicine. Journal of physics D: Applied physics, 2009. 42(22): p. 224003. https://doi.org/10.1088/0022-3727/42/22/224003
13.    Wang, W., Y. Jing, S. He, J.-P. Wang and J.-P. Zhai, Surface modification and bioconjugation of FeCo magnetic nanoparticles with proteins. Colloids and Surfaces B: Biointerfaces, 2014. 117: p. 449-456. https://doi.org/10.1016/j.colsurfb.2013.11.050
14.    Zhang, X., L. Xue, J. Wang, Q. Liu, J. Liu, Z. Gao and W. Yang, Effects of surface modification on the properties of magnetic nanoparticles/PLA composite drug carriers and in vitro controlled release study. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2013. 431: p. 80-86. https://doi.org/10.1016/j.colsurfa.2013.04.021
15.    Chomoucka, J., J. Drbohlavova, D. Huska, V. Adam, R. Kizek and J. Hubalek, Magnetic nanoparticles and targeted drug delivering. Pharmacological Research, 2010. 62(2): p. 144-149. https://doi.org/10.1016/j.phrs.2010.01.014
16.    Wu, W., Z. Wu, T. Yu, C. Jiang and W.-S. Kim, Recent progress on magnetic iron oxide nanoparticles: synthesis, surface functional strategies and biomedical applications. Science and Technology of Advanced Materials, 2016. https://doi.org/10.1088/1468-6996/16/2/023501
17.    Matuszak, J., J. Baumgartner, J. Zaloga, M. Juenet, A.E. Da Silva, D. Franke, G. Almer, I. Texier, D. Faivre and J.M. Metselaar, Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing. Nanomedicine, 2016. 11(6): p. 597-616. https://doi.org/10.2217/nnm.15.216
18.    Oumzil, K., M.A. Ramin, C. Lorenzato, A. Hémadou, J. Laroche, M.J. Jacobin-Valat, S. Mornet, C.-E. Roy, T. Kauss and K. Gaudin, Solid Lipid Nanoparticles for imageguided therapy of atherosclerosis. Bioconjugate chemistry, 2016. 27(3): p. 569-575. https://doi.org/10.1021/acs.bioconjchem.5b00590
19.    Zhao, S., Y. Zhang, Y. Han, J. Wang and J. Yang, Preparation and Characterization of cisplatin magnetic solid lipid nanoparticles (MSLNs): effects of loading procedures of Fe3O4 nanoparticles. Pharmaceutical research, 2015. 32(2): p. 482-491. https://doi.org/10.1007/s11095-014-1476-2
20.    Lodhia, J., G. Mandarano, N. Ferris, P. Eu and S. Cowell, Development and use of iron oxide nanoparticles (Part 1): Synthesis of iron oxide nanoparticles for MRI. Biomed Imaging Interv J, 2010. 6(2): p. e12. https://doi.org/10.2349/biij.6.2.e12
21.    Afshari, M., K. Derakhshandeh and L. Hosseinzadeh, Characterisation, cytotoxicity and apoptosis studies of methotrexate-loaded PLGA and PLGA-PEG nanoparticles. Journal of microencapsulation, 2014. 31(3): p. 239-245. https://doi.org/10.3109/02652048.2013.834991
22.    Derakhshandeh, K., M. Soheili, S. Dadashzadeh and R. Saghiri, Preparation and in vitro characterization of 9-nitrocamptothecin-loaded long circulating nanoparticles for delivery in cancer patients. Int J Nanomedicine, 2010. 5(1): p. 1-9. https://doi.org/10.2147/IJN.S11586
23.    Sahoo, J., P. Murthy and S. Biswal, Formulation of sustained-release dosage form of verapamil hydrochloride by solid dispersion technique using Eudragit RLPO or kollidon® SR. AAPS PharmSciTech, 2009. 10(1): p. 27-33. https://doi.org/10.1208/s12249-008-9175-0
24.    Lokhandwala, H., A. Deshpande and S. Deshpande, Kinetic modeling and dissolution profiles comparison: an overview. Int. J. Pharm. Bio. Sci, 2013. 4(1): p. 728-73.
25.    Ding, J., Chen, G., ,Guofang Chen, G., and  Gu, M.    One-Pot Synthesis of Epirubicin-Capped Silver Nanoparticles and Their Anticancer Activity against Hep G2 Cells, Pharmaceutics. 2019. 11(3): p.123 https://doi.org/10.3390/pharmaceutics11030123
26.    Mikhaylova, M., D.K. Kim, N. Bobrysheva, M. Osmolowsky, V. Semenov, T. Tsakalakos and M. Muhammed, Superparamagnetism of magnetite nanoparticles: dependence on surface modification. Langmuir, 2004. 20(6): p. 2472-2477. https://doi.org/10.1021/la035648e
27.    Chen, H., L.Q. Xie, J. Qin, Y. Jia, X. Cai, W. Nan, W. Yang, F. Lv and Q.Q. Zhang, Surface modification of PLGA nanoparticles with biotinylated chitosan for the sustained in vitro release and the enhanced cytotoxicity of epirubicin. Colloids and Surfaces B: Biointerfaces, 2016. 138: p. 1-9. https://doi.org/10.1016/j.colsurfb.2015.11.033
28.    Markides, H., M. Rotherham and A. El Haj, Biocompatibility and toxicity of magnetic nanoparticles in regenerative medicine. Journal of Nanomaterials, 2012. 2012. https://doi.org/10.1155/2012/614094
29.    Jain, T.K., M.K. Reddy, M.A. Morales, D.L. Leslie-Pelecky and V. Labhasetwar, Biodistribution, clearance, and biocompatibility of iron oxide magnetic nanoparticles in rats. Molecular pharmaceutics, 2008. 5(2): p. 316-327. https://doi.org/10.1021/mp7001285
30.    Pang, X., Cui, F., Tian, J., Chen, J., Zhou, J., Zhou, W. Preparation and characterization of magnetic solid lipid nanoparticles loaded with ibuprofen. Asian Journal of Pharmaceutical Science, 2009, 4, p.132-137.
31.    Oliveira, R.R., Carrião, M., Pacheco, M.T., Branquinho, L.C., de Souza, A.L.R., Bakuzis, A., Lima, E.M. Triggered release of paclitaxel from magnetic solid lipid nanoparticles by magnetic hyperthermia. Materials Science and Engineering: A , 2018, 92, p.547-553. https://doi.org/10.1016/j.msec.2018.07.011
32.    Abidi, H., Ghaedi, M., Rafiei, A., Jelowdar, A., Salimi, A., Asfaram, A., Ostovan, A. Magnetic solid lipid nanoparticles co-loaded with albendazole as an anti-parasitic drug: Sonochemical preparation, characterization, and in vitro drug release. Journal of Molecular Liquid, 2018, 268, p.11-18. https://doi.org/10.1016/j.molliq.2018.06.116
33.    Ahmadifard, Z., Ahmeda, A., Rasekhian, M., Moradi, S., Arkan, E. Chitosan-coated magnetic solid lipid nanoparticles for controlled release of letrozole. Journal of Drug Delivery Science and Technology. 2020, 57, p.101621. https://doi.org/10.1016/j.jddst.2020.101621
34.    Grillone, A., Battaglini, M., Moscato, S., Mattii, L., Fernández, C.D.J., Scarpellini, A., Giorgi, M., Sinibaldi, E., Ciofani, G. Nutlin-loaded magnetic solid lipid nanoparticles for targeted glioblastoma treatment. Nanomedicine 2019, 14, p. 727-752. https://doi.org/10.2217/nnm-2018-0436
35.    Rocha, C.V., da Silva, M.C., Bañobre-López, M., Gallo, J. (Para) magnetic hybrid nanocomposites for dual MRI detection and treatment of solid tumours. Chemical Communication. 2020, 56, p. 8695-8698. https://doi.org/10.1039/D0CC03020A
36.    Abakumov, M.A., Semkina, A.S., Skorikov, A.S., Vishnevskiy, D.A., Ivanova, A.V., Mironova, E., Davydova, G.A., Majouga, A.G., Chekhonin, V.P. Toxicity of iron oxide nanoparticles: Size and coating effects. Journal of Biochemistry Molecular Toxicology. 2018, 32, p.e22225. https://doi.org/10.1002/jbt.22225
37.    Hsu, M.-H., Su, Y.-C. Iron-oxide embedded solid lipid nanoparticles for magnetically controlled heating and drug delivery. biomedical microdevices journal, 2008, 10, p.785-793. https://doi.org/10.1007/s10544-008-9192-5
 
Nanomedicine Research Journal
Volume 9, Issue 3
Autumn 2024
Pages 264-273