Nanomedicine Research Journal

Nanomedicine Research Journal

MoS2 Improves the Function of Pancreatic β-cells in Type 2 Diabetes Mellitus

Document Type : Original Research Article

Authors
Fatemeh Ghafari 1
Tahereh Foroutan 1
Marzieh Salimi 2
Rambod Norouzi 3
1 Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
2 Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran
3 Department of Molecular Biosciences, Autonomous University of Madrid, Madrid, Spain
10.22034/nmrj.2024.03.002
Abstract
In recent years, anti-cancer and stem cell differentiation properties have been reported for some molybdenum derivatives such as molybdenum disulfide (MoS2). Diabetes mellitus as a chronic metabolic disorder has symptoms such as insufficient insulin secretion or insulin dysfunction due to β cell destruction. Each of the current diabetes treatment methods has limitations. In the present research the effects of MoS2–PEG on survival rate, expression of genes involved in glucose metabolism, and insulin secretion in diabetic RIN-5F β cell was studied. Synthesized MoS2-PEG nanosheets was used for possible effects of MoS2 on RIN-5F cells induced by STZ (40 mM). MTT assay, RT-PCR, and hormonal analyses were used to investigate the anti-toxicity effect of MoS2 and its role in improving the function of diabetic RIN-5F cells. The results showed that MoS2 is biocompatible and non-toxic at the dose used in the present study and significantly increased the expression of GLUT4, GCK, and INS genes involved in glucose metabolism as well as anti- apoptotic gene BCL2 in the diabetic RIN-5F cells. Also, treatment with MoS2 increased insulin secretion in diabetic RIN-5F cells. It could be concluded that MoS2 –PEG represents a protective role in the diabetic cells and significantly improve the treatment of diabetic cells mouse model. These results demonstrate the increased expression genes involved in glucose metabolism in pancreatic damaged cells.
Keywords
MoS2
Diabetes
Glucose metabolism genes
BCL2
Nanomaterial

Subjects

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Nanomedicine Research Journal
Volume 9, Issue 3
Autumn 2024
Pages 243-250