Because of their adjustable surface chemistry, high surface-to-volume ratio, and customizable particle size, nanotechnology-based delivery methods offer potent weapons against drug-resistant microbial diseases. In this regard, ionic gelation was used to create chitosan–meropenem nanoparticles (CS–MEM NPs), which were thoroughly described as a viable antibacterial platform against the growing threat of multidrug-resistant (MDR) infections. Successful drug loading within the chitosan matrix was validated through the nanoparticles’ homogeneous nanoscale diameters, positive zeta potential, and great encapsulation efficiency. Their physicochemical and structural attributes were further validated using DLS, SEM, and FTIR analyses. Because of their adjustable surface chemistry, high surface-to-volume ratio, and customizable particle size, nanotechnology-based delivery methods offer potent weapons against drug-resistant microbial diseases. In this regard, ionic gelation was used to create chitosan–meropenem nanoparticles (CS–MEM NPs), which were thoroughly described as a viable antibacterial platform against the growing threat of multidrug-resistant (MDR) infections. Successful drug loading within the chitosan matrix was validated through the nanoparticles’ homogeneous nanoscale diameters, positive zeta potential, and great encapsulation efficiency. DLS, SEM, and FTIR investigations were used to further evaluate their physicochemical and structural characteristics. These results suggest that chitosan has antifungal properties beyond drug delivery. All things considered, CS-MEM nanoparticles are an efficient nano-delivery method that can improve antimicrobial efficacy, lower dosage requirements, and combat growing antimicrobial resistance. To verify their safety, viability, and translational potential, more in vivo and clinical study is necessary.