Document Type : Original Research Article
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University Tehran, Iran
Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Objective(s): Carbon dots (C-dots) are an emerging class of engineered nanomaterials with broad applications in medicine, bio-imaging, sensing, electronic devices, and catalysis. The study aimed to synthesize carbon nanoparticles with antibacterial therapeutic properties against clindamycin-resistant Staphylococcus aureus and ciprofloxacin-resistant Klebsiella pneumonia strains.
Methods: The C-dots were prepared by a hydrothermal method. Then the synthesized carbon dot were characterized by UV-visible spectroscopy, dynamic light scattering, Fourier transform infrared spectroscopy and transmission electron microscopy. The minimum inhibitory concentration of C-dots was evaluated by the micro-broth dilution method. Antibiotic susceptibility testing was performed using the disk diffusion method.
Results: The C-dots significantly reduced S. aureus and K. pneumoniae strains growth when compared to untreated bacteria (control; P < 0.05). Therefore, the minimum inhibitory concentration (MIC) of C-dots for clindamycin-resistant S. areus and ciprofloxacin-resistant K. pneumoniae strains were 500 and 250 µg/ml, respectively.
The survival percentage of S. areus and K. pneumoniae decreased to 48.05% and 11.6% respectively after treatment with 250 μg/ml C-dots. However, the viability of bacteria decreased to 3.8% and 2.5% at the concentration of 500 μg/ml.
Conclusions: The results show that by producing antibacterial drugs at the nanoscale, C-dots are a promising new approach to improve the effectiveness of treating infections caused by antibiotic-resistant bacterial strains.