Hair-Regrowing Potential of Minoxidil Nanocrystal Structure versus Rosemary's Hydroethanolic Extract on C57BL/6 Mice

Document Type : Original Research Article


1 Department of Biology, Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran

2 Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

5 Department of Pharmaceutics, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran



This study aimed to enhance the effectiveness and water solubility of Minoxidil (MXD) by producing its nanocrystal structure, which improves its vasodilator properties and promotes hair growth. In the current study, the hair growth-stimulating activity of the MXD nanoparticles (MXD-NPs) was compared with the hydroethanolic rosemary (RSY) extract on the C57BL/6 mice. The MXD-NPs were produced through a bead mill and ultrasonic process and characterized using various techniques. The cytotoxicity of MXD-NPs was studied on human dermal fibroblasts, and their hair growth-stimulating activity was analyzed in C57BL/6 mice. The results showed that MXD-NPs significantly increased the hair growth rate in mice compared to commercial MXD and hydroethanolic rosemary extract as they were delivered safely and specifically to the target pilosebaceous follicles. The follicular uptake of MXD-NPs was also increased compared to commercial MXD, leading to improved pilosebaceous follicle re-growth and hair growth in treated mice. Therefore, MXD-NPs have the potential to be a safe and efficient iso-formulation structure for hair growth promotion.

Graphical Abstract

Hair-Regrowing Potential of Minoxidil Nanocrystal Structure versus Rosemary's Hydroethanolic Extract on C57BL/6 Mice


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