Nanomedicine Research Journal

Nanomedicine Research Journal

The study of biocompatibility of electrospun polycaprolactone/gelatin scaffold containing double layer hydroxide nanoparticles on L929 mouse fibroblast cells

Document Type : Original Research Article

Authors
Mahsa Mirzavand 1
Tahereh Foroutan 1
Seyedeh Sara Shafiei 2
1 Department of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
2 Department of Stem Cells and Regenerative Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
10.22034/nmrj.2024.04.009
Abstract
Objective(s): Nanofibrous scaffolds provide an environment similar to the body's extracellular matrix for cells, which affects cell morphology, adhesion, proliferation, and function.  The present study aimed to investigate the biocompatibility properties of electrospun polycaprolactone-gelatin (PCL/Gel) scaffold containing layered double hydroxide (LDH) nanoparticles on mouse L929 fibroblast cells. 
Methods: In this study, LDH nanoparticles were synthesized by co-precipitation method. A scaffold made of polycaprolactone and gelatin with 1% wt of optimized nanoclay was fabricated using electrospinning method. The synthesized nanoparticles were evaluated by XRD method and electron microscopy study. 2×104 L929 fibroblast cells were seeded on the scaffolds for 3 to 5 days. Then, different grades of ethanol were used to dehydrate the scaffolds. After drying the scaffolds, scanning electron microscopy was used to characterize and examine the fixed cells, and MTT test was used to evaluate the toxicity of the scaffolds.
Results: The results of the present study showed that with the addition of LDH reduced the fiber diameter and increased the biocompatibility of the scaffold after culturing mouse L929 fibroblast cells. Also, scanning electron microscope images indicated better interaction and proliferation of L929 cells on PCL/Gel/LDH compared to the PCL/Gel group.
Conclusions: Adding LDH to PCL/Gel nanofibers is effective on the adhesion and viability of cells.
Keywords
polycaprolactone/gelatin
layered double hydroxide nanoparticles
fibroblast cells
L929

Subjects

1.    Elsner JJ, et al. Novel biodegradable composite wound dressings with controlled release of antibiotics: Results in a guinea pig burn model. Burns. 2011;37(5):896-904. https://doi.org/10.1016/j.burns.2011.02.010
2.    Doi Y, Steinbuchel A, Chen G. Biopolymers, Polyesters III - Applications and Commercial Products. Wiley-VCH; 2002. 
3.    Pazyar N, et al. Skin wound healing and phytomedicine: a review. Skin Pharmacol Physiol. 2014;27(6):303-10. https://doi.org/10.1159/000357477 
4.    Ghasemi-Mobarakeh L, et al. Electrospun poly(ε-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials. 2008;29(34):4532-9. https://doi.org/10.1016/j.biomaterials.2008.08.007 
5.    Khandwekar AP, et al. In vivo modulation of foreign body response on polyurethane by surface entrapment technique. J Biomed Mater Res A. 2010;94(2):227-52. https://doi.org/10.1002/jbm.a.32852 
6.    Hajiali F, Tajbakhsh S, Shojaei A. Fabrication and properties of polycaprolactone composites containing calcium phosphate-based ceramics and bioactive glasses in bone tissue engineering: a review. Polym Rev. 2018;58(1):164-207. https://doi.org/10.1080/15583724.2017.1332640 
7.    Liu X, Won Y, Ma PX. Surface modification of interconnected porous scaffolds. J Biomed Mater Res A. 2005;74(1):84-91. https://doi.org/10.1002/jbm.a.30367 
8.    Alihosseini F. Plant-based compounds for antimicrobial textiles. In: Antimicrobial Textiles. Elsevier; 2016:155-95. https://doi.org/10.1016/B978-0-08-100576-7.00010-9 
9.    Ghasemi-Mobarakeh L, et al. Electrospun poly(ε-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials. 2008;29(34):4532-9. https://doi.org/10.1016/j.biomaterials.2008.08.007 
10.    Mattioli-Belmonte M, et al. Tuning polycaprolactone-carbon nanotube composites for bone tissue engineering scaffolds. Mater Sci Eng C. 2012;32(2):152-9. https://doi.org/10.1016/j.msec.2011.10.010 
11.    Jin W, et al. Biocompatible hydrotalcite nanohybrids for medical functions. Minerals. 2020;10:172. https://doi.org/10.3390/min10020172 
12.    Lv F, et al. Layered double hydroxide assemblies with controllable drug loading capacity and release behavior as well as stabilized layer-by-layer polymer multilayers. ACS Appl Mater Interfaces. 2015;7(34):19104-11. https://doi.org/10.1021/acsami.5b04569 
13.    Senapati S, et al. Layered double hydroxides as effective carrier for anticancer drugs and tailoring of release rate through interlayer anions. J Control Release. 2016;224:186-98. https://doi.org/10.1016/j.jconrel.2016.01.016 
14.    Baradaran T, et al. Poly(ε-caprolactone)/layered double hydroxide microspheres-aggregated nanocomposite scaffold for osteogenic differentiation of mesenchymal stem cell. Mater Today Commun. 2020;24:100913. https://doi.org/10.1016/j.mtcomm.2020.100913 
15.    Ahmadi S, et al. Electrospun nanofibrous scaffolds of polycaprolactone/gelatin reinforced with layered double hydroxide nanoclay for nerve tissue engineering applications. ACS Omega. 2022;7(22):18519-31. https://doi.org/10.1021/acsomega.2c02863 
16.    Mohamadi F, et al. Electrospun nerve guide scaffold of poly(ε-caprolactone)/collagen/nanobioglass: an in vitro study in peripheral nerve tissue engineering. J Biomed Mater Res A. 2017;105(7):1960-72. https://doi.org/10.1002/jbm.a.36068
Nanomedicine Research Journal
Volume 9, Issue 4
Autumn 2024
Pages 424-431