Nanomedicine Research Journal

Nanomedicine Research Journal

Investigation of Effective Parameters on Aptamer-Based Electrochemical Sensor for Rapid Detection of Tetracycline Residues in Chicken Ham

Document Type : Original Research Article

Authors
Somayeh Mohammadi 1
Rezvan Mousavi Nadushan 1
Kamran Pooshang Bagheri 2
1 Department of Food Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Venom and Biotherapeutics Molecules Lab, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
10.22034/nmrj.2024.04.010
Abstract
This study investigates the impact of key parameters, including aptamer concentration, aptamer incubation time, tetracycline (Tet) incubation time, pH, and temperature, on the peak current of an aptasensor designed for Tet detection. The aptasensor was developed by immobilizing a Tet aptamer onto gold nanoparticles (AuNPs) electrodeposited on an electrospun carbon nanofibers (CNFs). The integration of CNFs and AuNPs enhances the sensor's performance by increasing the surface area and improving conductivity. The results show that aptamer concentration, aptamer incubation time, incubation time of Tet, pH, and temperature affect the peak current of the aptasensor, demonstrating the importance as effective parameters in the preparation of biosensors.
Keywords
Tetracycline
Electrochemical aptasensor
Gold nanoparticles

Subjects

1.    Mohammadi S, et al. Nanoarchitectonics of electrochemical aptasensor based on electrospun carbon nanofibers and gold nanoparticles for tetracycline detection in chicken ham. Appl Phys A. 2023;129(3):176. https://doi.org/10.1007/s00339-023-06416-4 
2.    Alfredsson G, et al. Simple and rapid screening and confirmation of tetracyclines in honey and egg by a dipstick test and LC-MS/MS. Anal Chim Acta. 2005;529(1-2):47-51. https://doi.org/10.1016/j.aca.2004.08.050
3.    Liu F, et al. Simultaneous screening and determination of eight tetracycline antibiotics illegally adulterated in herbal preparations using HPLC-DAD combined with LC-MS-MS. Chromatographia. 2018;81:303-14. https://doi.org/10.1007/s10337-017-3450-8
4.    Jadhav MR, et al. A unified approach for high-throughput quantitative analysis of the residues of multi-class veterinary drugs and pesticides in bovine milk using LC-MS/MS and GC-MS/MS. Food Chem. 2019;272:292-305. https://doi.org/10.1016/j.foodchem.2018.08.033
5.    Ebrahimi Vafaye S, et al. Investigation of effective parameters on electrochemical aptasensor for detection of Penicillin antibiotic. Nanomed Res J. 2021;6(1):73-8.
6.    Geravand M, et al. Nanoarchitectonics of aptamer-based electrochemical biosensor utilizing electrospun carbon nanofibers and gold nanoparticles for Acinetobacter baumannii detection. Microchem J. 2024;200:110437. https://doi.org/10.1016/j.microc.2024.110437
7.    Seifi N, et al. Anti-cancerous effect and biological evaluation of green synthesized Selenium nanoparticles on MCF-7 breast cancer and HUVEC cell lines. Nanomed Res J. 2023;8(4):373-82.
8.    Niknam S, et al. Tungsten disulfide nanomaterials (WS2 NM) application in biosensors and nanomedicine: a review. Nanomed Res J. 2022;7(3):214-26.
9.    Mansoori R, et al. Green Synthesis of Gold Nanoparticles using Melissa Officinalis L. Aqueous Extract: Optimization, Antioxidant Activity and In Vitro Cell Viability. Nanomed Res J. 2023;8(4):383-92. https://doi.org/10.2139/ssrn.4504359
10.    Salimi M, et al. Electrochemical immunosensor based on carbon nanofibers and gold nanoparticles for detecting anti-Toxoplasma gondii IgG antibodies. Microchim Acta. 2023;190(9):367. https://doi.org/10.1007/s00604-023-05928-3
11.    He X, et al. Sensitization Strategies of Lateral Flow Immunochromatography for Gold Modified Nanomaterials in Biosensor Development. Int J Nanomedicine. 2023;18:7847-63. https://doi.org/10.2147/IJN.S436379
12.    Behzad F, et al. Recent advances in gold nanoparticle-based colorimetric aptasensors for chemical and biological analyses. Bioanalysis. 2022;14(23):1509-24. https://doi.org/10.4155/bio-2022-0209
13.    Huang J, Liu Y, You T. Carbon nanofiber based electrochemical biosensors: A review. Anal Methods. 2010;2(3):202-11. https://doi.org/10.1039/b9ay00312f
14.    Banan K, et al. Molecularly imprinted electrochemical sensor based on carbon nanofibers for Amiodarone determination. Microchem J. 2024;200:110365. https://doi.org/10.1016/j.microc.2024.110365
15.    Adabi M, Esnaashari SS, Adabi M. An electrochemical immunosensor based on electrospun carbon nanofiber mat decorated with gold nanoparticles and carbon nanotubes for the detection of breast cancer. J Porous Mater. 2021;28(2):415-21. https://doi.org/10.1007/s10934-020-01004-w
16.    Iftikhar FJ, et al. Advancements in nanofiber-based electrochemical biosensors for diagnostic applications. Biosensors. 2023;13(4):416. https://doi.org/10.3390/bios13040416
17.    Qin R, et al. Gold Nanoparticle-Modified Electrodes for Electrochemical Sensing of Ammonia Nitrogen in Water. ACS Appl Nano Mater. 2023;7(1):577-93. https://doi.org/10.1021/acsanm.3c04757
18.    Narayan C, Veeramani S, Thiel WH. Optimization of RNA aptamer SELEX methods: improved aptamer transcript 3’-end homogeneity, PAGE purification yield, and target-bound aptamer RNA recovery. Nucleic Acid Ther. 2022;32(1):74-80. https://doi.org/10.1089/nat.2021.0060
19.    Kohlberger M, Gadermaier G. SELEX: Critical factors and optimization strategies for successful aptamer selection. Biotechnol Appl Biochem. 2022;69(5):1771-92. https://doi.org/10.1002/bab.2244
20.    Malecka-Baturo K, et al. Electrochemical Biosensor Designed to Distinguish Tetracyclines Derivatives by ssDNA Aptamer Labelled with Ferrocene. Int J Mol Sci. 2022;23(22):13785. https://doi.org/10.3390/ijms232213785
21.    Zhang Z, Karimi-Maleh H. Label-free electrochemical aptasensor based on gold nanoparticles/titanium carbide MXene for lead detection with its reduction peak as index signal. Adv Compos Hybrid Mater. 2023;6(2):68. https://doi.org/10.1007/s42114-023-00652-1
Nanomedicine Research Journal
Volume 9, Issue 4
Autumn 2024
Pages 432-436