The green-synthesized metal and metal oxide nanoparticles in dental implant applications

Document Type : Review Paper


1 School of Dentistry_ International Campus Tehran University of Medical Sciences Tehran Iran

2 Department of Orthodontics, School of Dentistry, Alborz University of Medical Sciences, Karaj, Iran

3 Assistant Professor,Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran

4 School of Dentistry, Qazvin university of medical sciences, Qazvin, Iran

5 Resident of Orthodontics at Department of Orthodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

6 Department of periodontics, School of Dentistry,Tabriz University of Medical Sciences,Tabriz,Iran


Surface-modifying biomaterials have the potential to improve both the performance and durability of dental implantable products that are currently in use. Dental implants' surfaces may be modified to improve their biocompatibility and other biologically significant characteristics using metal and metal oxide nanoparticle coatings. The toxicity of the materials used in the synthesis, the requirement for high temperature and energy, and the high cost are just a few of the factors that restrict the use of the various physical and chemical methods for the synthesis of metal nanoparticles. Though, these restrictions can be overcome by developing substitute synthetic approaches that are similar to Green Synthesis and have proven to be more eco-friendly and less toxic, including the use of algae, microorganisms, and plants. Metal ions can be readily reduced into nanoparticles by plants' biomolecules, secondary metabolites, and coenzymes. Although still in its infancy, the use of metal nanoparticles produced through green synthesis in dental implants has the potential to open up new avenues for enhancing the caliber of these goods.


Main Subjects

  1. Abdollahii, S., et al., Adverse Effects of some of the Most Widely used Metal Nanoparticles on the Reproductive System. Journal of Infertility and Reproductive Biology, 2020. 8(3): p. 22-32.
  2. Huston, M., et al., Green synthesis of nanomaterials. Nanomaterials, 2021. 11(8): p. 2130.  
  3. Prasad, R.D., et al., A review on concept of nanotechnology in veterinary medicine. ES Food & Agroforestry, 2021. 4: p. 28-60.  
  4. Salem, S.S. and A. Fouda, Green synthesis of metallic nanoparticles and their prospective biotechnological applications: an overview. Biological Trace Element Research, 2021. 199(1): p. 344-370.  
  5. Steigenga, J.T., et al., Dental implant design and its relationship to long-term implant success. Implant dentistry, 2003. 12(4): p. 306-317.  
  6. Thakral, G., et al., Nanosurface-the future of implants. Journal of Clinical and Diagnostic Research: JCDR, 2014. 8(5): p. ZE07.  
  7. Anil, S., et al., Dental implant surface enhancement and osseointegration. Implant dentistry-a rapidly evolving practice, 2011: p. 83-108.  
  8. Di Spirito, F., et al., Inflammatory, Reactive, and Hypersensitivity Lesions Potentially Due to Metal Nanoparticles from Dental Implants and Supported Restorations: An Umbrella Review. Applied Sciences, 2022. 12(21): p. 11208.  
  9. Parekh, R.B., O. Shetty, and R. Tabassum, Surface modifications for endosseous dental implants. Int J Oral Implantol Clin Res, 2012. 3(3): p. 116-121.  
  10. Krueger, A. and D. Lang, Functionality is key: recent progress in the surface modification of nanodiamond. Advanced Functional Materials, 2012. 22(5): p. 890-906.  
  11. Yin, C., et al., Effects of the micro-nano surface topography of titanium alloy on the biological responses of osteoblast. Journal of Biomedical Materials Research Part A, 2017. 105(3): p. 757-769.  
  12. Yazdani, J., et al., A short view on nanohydroxyapatite as coating of dental implants. Biomedicine & Pharmacotherapy, 2018. 105: p. 553-557.  
  13. Fernández-Lizárraga, M., et al., Evaluation of the biocompatibility and osteogenic properties of metal oxide coatings applied by magnetron sputtering as potential biofunctional surface modifications for orthopedic implants. Materials, 2022. 15(15): p. 5240.  
  14. Mahajan, A. and S. Sidhu, Surface modification of metallic biomaterials for enhanced functionality: a review. Materials technology, 2018. 33(2): p. 93-105.  
  15. Anu, K., et al., Wet biochemical synthesis of copper oxide nanoparticles coated on titanium dental implants. Int. J. Adv. Res. Sci. Eng. Technol, 2016. 3: p. 1191-1194. 
  16. Maleki Dizaj, S., et al., Calcium carbonate nanoparticles as cancer drug delivery system. Expert opinion on drug delivery, 2015. 12(10): p. 1649-1660.  
  17. Xu, V.W., et al., Application of copper nanoparticles in dentistry. Nanomaterials, 2022. 12(5): p. 805.  
  18. El-Morsy, M., et al., Optimizing the mechanical and surface topography of hydroxyapatite/Gd2O3/graphene oxide nanocomposites for medical applications. Journal of Saudi Chemical Society, 2022. 26(3): p. 101463.  
  19. Barkalina, N., et al., Nanotechnology in reproductive medicine: emerging applications of nanomaterials. Nanomedicine: Nanotechnology, Biology and Medicine, 2014. 10(5): p. e921-e938.  
  20. León-Silva, S., F. Fernández-Luqueño, and F. López-Valdez, Silver nanoparticles (AgNP) in the environment: a review of potential risks on human and environmental health. Water, Air, & Soil Pollution, 2016. 227(9): p. 1-20.  
  21. Sarani, M., et al., Study of in vitro cytotoxic performance of biosynthesized α-Bi2O3 NPs, Mn-doped and Zn-doped Bi2O3 NPs against MCF-7 and HUVEC cell lines. journal of materials research and technology, 2022. 19: p. 140-150.  
  22. Cauerhff, A. and G.R. Castro, Bionanoparticles, a green nanochemistry approach Electronic Journal of Biotechnology, vol. 16, núm. 3, 2013, pp. 1-10 Pontificia Universidad Católica de Valparaíso Valparaíso, Chile. Electronic Journal Of Biotechnology, 2013. 16(3): p. 1-10.  
  23. Duan, H., D. Wang, and Y. Li, Green chemistry for nanoparticle synthesis. Chemical Society Reviews, 2015. 44(16): p. 5778-5792.  
  24. Pal, S.L., et al., Nanoparticle: An overview of preparation and characterization. Journal of applied pharmaceutical science, 2011(Issue): p. 228-234.  
  25. Abdelhameed, R.M., M. El-Shahat, and H.E. Emam, Employable metal (Ag & Pd)@ MIL-125-NH2@ cellulose acetate film for visible-light driven photocatalysis for reduction of nitro-aromatics. Carbohydrate polymers, 2020. 247: p. 116695.  
  26. Ahmed, H.B. and H.E. Emam, Seeded growth core-shell (Ag-Au-Pd) ternary nanostructure at room temperature for potential water treatment. Polymer Testing, 2020. 89: p. 106720.  
  27. Souza, J.A., et al., Green synthesis of silver nanoparticles combined to calcium glycerophosphate: antimicrobial and antibiofilm activities. Future microbiology, 2018. 13(3): p. 345-357.  
  28. Ali, Z., et al., Oral health-related quality of life after prosthodontic treatment for patients with partial edentulism: A systematic review and meta-analysis. The Journal of prosthetic dentistry, 2019. 121(1): p. 59-68. e3.  
  29. Castner, D.G. and B.D. Ratner, Biomedical surface science: Foundations to frontiers. Surface Science, 2002. 500(1-3): p. 28-60.  
  30. Abrahamsson, I., et al., Early bone formation adjacent to rough and turned endosseous implant surfaces: an experimental study in the dog. Clinical oral implants research, 2004. 15(4): p. 381-392.  
  31. Shibli, J.A., et al., Influence of implant surface topography on early osseointegration: a histological study in human jaws. Journal of Biomedical Materials Research Part B: Applied Biomaterials: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 2007. 80(2): p. 377-385.  
  32. Espinar-Escalona, E., et al., Roughness and wettability effect on histological and mechanical response of self-drilling orthodontic mini-implants. Clinical oral investigations, 2016. 20(5): p. 1115-1120.  
  33. Gaviria, L., et al., Current trends in dental implants. Journal of the Korean Association of Oral and Maxillofacial Surgeons, 2014. 40(2): p. 50.  
  34. Pachauri, P., L.R. Bathala, and R. Sangur, Techniques for dental implant nanosurface modifications. The journal of advanced prosthodontics, 2014. 6(6): p. 498-504.  
  35. Mendonça, G., et al., Advancing dental implant surface technology-from micron-to nanotopography. Biomaterials, 2008. 29(28): p. 3822-3835.  
  36. Nayar, S., S. Bhuminathan, and J. Muthuvignesh, Upsurge of nanotechnology in dentistry and dental implants. Indian journal of multidisciplinary dentistry, 2011. 1(5).  
  37. Lavenus, S., et al., Cell interaction with nanopatterned surface of implants. Nanomedicine, 2010. 5(6): p. 937-947.  
  38. Le Guéhennec, L., et al., Surface treatments of titanium dental implants for rapid osseointegration. Dental materials, 2007. 23(7): p. 844-854.  
  39. Lavenus, S., G. Louarn, and P. Layrolle, Nanotechnology and dental implants. International journal of biomaterials, 2010. 2010.  
  40. Christenson, E.M., et al., Nanobiomaterial applications in orthopedics. Journal of orthopaedic research, 2007. 25(1): p. 11-22.  
  41. Geesink, R.G., K. de Groot, and C.P. Klein, Chemical implant fixation using hydroxyl-apatite coatings: the development of a human total hip prosthesis for chemical fixation to bone using hydroxyl-apatite coatings on titanium substrates. Clinical Orthopaedics and Related Research®, 1987. 225: p. 147-170.  
  42. Zhao, G., et al., Osteoblast‐like cells are sensitive to submicron‐scale surface structure. Clinical oral implants research, 2006. 17(3): p. 258-264.  
  43. Balasundaram, G., M. Sato, and T.J. Webster, Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD. Biomaterials, 2006. 27(14): p. 2798-2805.  
  44. Elias, C.N. and L. Meirelles, Improving osseointegration of dental implants. Expert review of medical devices, 2010. 7(2): p. 241-256.  
  45. Khandaker, M., et al., Peen treatment on a titanium implant: effect of roughness, osteoblast cell functions, and bonding with bone cement. International Journal of Nanomedicine, 2016. 11: p. 585.  
  46. Sharifianjazi, F., et al., Hydroxyapatite consolidated by zirconia: applications for dental implant. Journal of Composites and Compounds, 2020. 2(2): p. 26-34.  
  47. Tosan, F., et al., Effects of doping metal nanoparticles in hydroxyapatite in Improving the physical and chemical properties of dental implants. Nanomedicine Research Journal, 2021. 6(4): p. 327-336.  
  48. Variola, F., et al., Nanoscale surface modifications of medically relevant metals: state-of-the art and perspectives. Nanoscale, 2011. 3(2): p. 335-353.  
  49. Xuereb, M., J. Camilleri, and N.J. Attard, Systematic review of current dental implant coating materials and novel coating techniques. International Journal of Prosthodontics, 2015. 28(1).  
  50. Dizaj, S.M., et al., Box-Behnken experimental design for preparation and optimization of ciprofloxacin hydrochloride-loaded CaCO3 nanoparticles. Journal of drug delivery science and technology, 2015. 29: p. 125-131.  
  51. Maleki Dizaj, S., Preparation and study of vitamin A palmitate microemulsion drug delivery system and investigation of co-surfactant effect. Journal of nanostructure in chemistry, 2013. 3: p. 1-6.  
  52. Maleki, S., et al., Calcium carbonate nanoparticles; potential applications in bone and tooth disorders. Pharmaceutical Sciences, 2015. 20(4): p. 175-182.  
  53. Chidambaranathan, A.S., K. Mohandoss, and M.K. Balasubramaniam, Comparative evaluation of antifungal effect of titanium, zirconium and aluminium nanoparticles coated titanium plates against C. albicans. Journal of clinical and diagnostic research: JCDR, 2016. 10(1): p. ZC56.  
  54. Shokuhfar, T., et al., Biophysical evaluation of cells on nanotubular surfaces: the effects of atomic ordering and chemistry. International journal of nanomedicine, 2014. 9: p. 3737.  
  55. Estrela, C., et al., Silver nanoparticles in resin luting cements: Antibacterial and physiochemical properties. Journal of Clinical and Experimental Dentistry, 2016. 8(4): p. e415.  
  56. Jain, S., et al., Nanotechnology: An emerging area in the field of dentistry. J Dent Sci, 2013. 10: p. 1-9.  
  57. Herter, P., et al., Silver‐enhanced colloidal‐gold labelling of rabbit kidney collecting‐duct cell surfaces imaged by scanning electron microscopy. Journal of microscopy, 1993. 171(2): p. 107-115.  
  58. Parnia, F., et al., Overview of nanoparticle coating of dental implants for enhanced osseointegration and antimicrobial purposes. Journal of Pharmacy & Pharmaceutical Sciences, 2017. 20: p. 148-160.  
  59. Javadhesari, S.M., S. Alipour, and M. Akbarpour, Biocompatibility, osseointegration, antibacterial and mechanical properties of nanocrystalline Ti-Cu alloy as a new orthopedic material. Colloids and Surfaces B: Biointerfaces, 2020. 189: p. 110889.  
  60. Vickers, N.J., Animal communication: when i'm calling you, will you answer too? Current biology, 2017. 27(14): p. R713-R715.  
  61. Kulshrestha, S., et al., A graphene/zinc oxide nanocomposite film protects dental implant surfaces against cariogenic Streptococcus mutans. Biofouling, 2014. 30(10): p. 1281-1294.  
  62. TK, C.M., Sau, AM Gole, CJ Orendorff, J, Gao. L. Gou, SE Hunyadi, T Li. J. Phys. Chem. B, 2005. 109: p. 13857.  
  63. Lee, J.-W., Manganese intoxication. Archives of neurology, 2000. 57(4): p. 597-599.  
  64. Gericke, M. and A. Pinches, Biological synthesis of metal nanoparticles. Hydrometallurgy, 2006. 83(1-4): p. 132-140.  
  65. Lenardão, E.J., et al., Green chemistry: the 12 principles of green chemistry and it insertion in the teach and research activities. Química Nova, 2003. 26: p. 123-129.  
  66. Tang, S.Y., et al., The 24 principles of green engineering and green chemistry:"IMPROVEMENTS PRODUCTIVELY". Green Chemistry, 2008. 10(3): p. 268-269.  
  67. Ahmad, A., et al., Extra-/intracellular biosynthesis of gold nanoparticles by an alkalotolerant fungus, Trichothecium sp. Journal of Biomedical Nanotechnology, 2005. 1(1): p. 47-53.  
  68. El‐Said, W.A., et al., Synthesis of metal nanoparticles inside living human cells based on the intracellular formation process. Advanced materials, 2014. 26(6): p. 910-918.  
  69. Kouhbanani, M.A.J., et al., The inhibitory role of synthesized Nickel oxide nanoparticles against Hep-G2, MCF-7, and HT-29 cell lines: the inhibitory role of NiO NPs against Hep-G2, MCF-7, and HT-29 cell lines. Green Chemistry Letters and Reviews, 2021. 14(3): p. 444-454.  
  70. Srivastava, P., et al., Synthesis of silver nanoparticles using haloarchaeal isolate Halococcus salifodinae BK3. Extremophiles, 2013. 17(5): p. 821-831.  
  71. Iravani, S., Green synthesis of metal nanoparticles using plants. Green Chemistry, 2011. 13(10): p. 2638-2650.  
  72. Mosleh-Shirazi, S., et al., Biosynthesis, simulation, and characterization of Ag/AgFeO2 core-shell nanocomposites for antimicrobial applications. Applied Physics A, 2021. 127(11): p. 1-8.  
  73. Bonatto, C.C. and L.P. Silva, Higher temperatures speed up the growth and control the size and optoelectrical properties of silver nanoparticles greenly synthesized by cashew nutshells. Industrial Crops and Products, 2014. 58: p. 46-54.  
  74. Silva, L.P., I.G. Reis, and C.C. Bonatto, Green synthesis of metal nanoparticles by plants: current trends and challenges. Green processes for nanotechnology, 2015: p. 259-275.  
  75. Corrêa, J.M., et al., Silver nanoparticles in dental biomaterials. International journal of biomaterials, 2015. 2015.  
  76. Kim, K.-J., et al., Antifungal effect of silver nanoparticles on dermatophytes. Journal of microbiology and biotechnology, 2008. 18(8): p. 1482-1484.  
  77. Panáček, A., et al., Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. The Journal of Physical Chemistry B, 2006. 110(33): p. 16248-16253.  
  78. Lok, C.-N., et al., Proteomic analysis of the mode of antibacterial action of silver nanoparticles. Journal of proteome research, 2006. 5(4): p. 916-924.  
  79. Morones, J.R., et al., The bactericidal effect of silver nanoparticles. Nanotechnology, 2005. 16(10): p. 2346.  
  80. Rai, M., A. Yadav, and A. Gade, Silver nanoparticles as a new generation of antimicrobials. Biotechnology advances, 2009. 27(1): p. 76-83.  
  81. Stickler, D.J., Biomaterials to prevent nosocomial infections: is silver the gold standard? Current Opinion in Infectious Diseases, 2000. 13(4): p. 389-393.  
  82. Durner, J., et al., Influence of silver nano-particles on monomer elution from light-cured composites. Dental Materials, 2011. 27(7): p. 631-636.  
  83. Flores, C., et al., Spontaneous adsorption of silver nanoparticles on Ti/TiO2 surfaces. Antibacterial effect on Pseudomonas aeruginosa. Journal of Colloid and Interface Science, 2010. 350(2): p. 402-408.  
  84. Lotfi, M., et al., Antimicrobial efficacy of nanosilver, sodium hypochlorite and chlorhexidine gluconate against Enterococcus faecalis. African Journal of Biotechnology, 2011. 10(35): p. 6799-6803.  
  85. Nam, K.-Y., In vitro antimicrobial effect of the tissue conditioner containing silver nanoparticles. The journal of advanced prosthodontics, 2011. 3(1): p. 20-24.  
  86. Gunputh, U.F., et al., Antibacterial properties of silver nanoparticles grown in situ and anchored to titanium dioxide nanotubes on titanium implant against Staphylococcus aureus. Nanotoxicology, 2020. 14(1): p. 97-110.  
  87. Pokrowiecki, R., et al., In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery. International journal of nanomedicine, 2017. 12: p. 4285.  
  88. Chladek, G., et al., Antifungal activity of denture soft lining material modified by silver nanoparticles-a pilot study. International Journal of Molecular Sciences, 2011. 12(7): p. 4735-4744.  
  89. Singh, P., et al., The development of a green approach for the biosynthesis of silver and gold nanoparticles by using Panax ginseng root extract, and their biological applications. Artificial cells, nanomedicine, and biotechnology, 2016. 44(4): p. 1150-1157.  
  90. Sreenivasagan, S., A.K. Subramanian, and S. Rajeshkumar, Assessment of antimicrobial activity and cytotoxic effect of green mediated silver nanoparticles and its coating onto mini-implants. Annals of Phytomedicine, 2020. 9(1): p. 207-212.  
  91. Rodrigues, M.C., et al., Biogenic synthesis and antimicrobial activity of silica-coated silver nanoparticles for esthetic dental applications. Journal of Dentistry, 2020. 96: p. 103327.  
  92. Sundeep, D., et al., Green synthesis and characterization of Ag nanoparticles from Mangifera indica leaves for dental restoration and antibacterial applications. Progress in biomaterials, 2017. 6(1): p. 57-66.  
  93. Majeed, S. and M. Khanday, Green synthesis of silver nanoparticles using bark extract of Salix alba and its antimicrobial effect against bacteria isolated from dental plaque. Oriental Journal of Chemistry, 2016. 32(3): p. 1611-1618.  
  94. De Giglio, E., et al., An innovative, easily fabricated, silver nanoparticle-based titanium implant coating: development and analytical characterization. Analytical and bioanalytical chemistry, 2013. 405(2): p. 805-816.  
  95. Narayanan, K.B. and N. Sakthivel, Green synthesis of biogenic metal nanoparticles by terrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatible agents. Advances in colloid and interface science, 2011. 169(2): p. 59-79.  
  96. Devi, L., et al., Synthesis, characterization and in vitro assessment of colloidal gold nanoparticles of Gemcitabine with natural polysaccharides for treatment of breast cancer. Journal of Drug Delivery Science and Technology, 2020. 56: p. 101565.  
  97. Elgamily, H.M., H.S. El-Sayed, and A. Abdelnabi, The antibacterial effect of two cavity disinfectants against one of cariogenic pathogen: An In vitro comparative study. Contemporary Clinical Dentistry, 2018. 9(3): p. 457.  
  98. Kesharwani, P., et al., Dendrimer-entrapped gold nanoparticles as promising nanocarriers for anticancer therapeutics and imaging. Progress in Materials Science, 2019. 103: p. 484-508.  
  99. Kesharwani, P., K. Jain, and N.K. Jain, Dendrimer as nanocarrier for drug delivery. Progress in Polymer Science, 2014. 39(2): p. 268-307.  
  100. Menon, S., S. Rajeshkumar, and S. Kumar, A review on biogenic synthesis of gold nanoparticles, characterization, and its applications. Resource-Effic Technol 3: 516-527. 2017.  
  101. Jadhav, K., et al., Phytosynthesis of gold nanoparticles: characterization, biocompatibility, and evaluation of its osteoinductive potential for application in implant dentistry. Materials Science and Engineering: C, 2018. 93: p. 664-670.  
  102. Wang, M. and L. Wang, Plant polyphenols mediated synthesis of gold nanoparticles for pain management in nursing care for dental tissue implantation applications. Journal of Drug Delivery Science and Technology, 2020. 58: p. 101753.  
  103. Dharman, S. and K. Rajeshkumar, Synthesis and Characterisation of Novel Turmeric Gold Nanoparticles and Evaluation of Its Anti-oxidant, Anti-Inflammatory, Antibacterial Activity for Application in Oral Mucositis-An In vitro Study. Int. J. Dentistry Oral Sci, 2021. 8(5): p. 2525-2532.  
  104. Emmanuel, R., et al., Antimicrobial efficacy of drug blended biosynthesized colloidal gold nanoparticles from Justicia glauca against oral pathogens: a nanoantibiotic approach. Microbial Pathogenesis, 2017. 113: p. 295-302.  
  105. Sharma, K. and C. Chauhan, Role of magnetic nanoparticle (MNPs) in cancer treatment: a review. Materials Today: Proceedings, 2021.  
  106. Suciu, M., et al., Applications of superparamagnetic iron oxide nanoparticles in drug and therapeutic delivery, and biotechnological advancements. Beilstein Journal of Nanotechnology, 2020. 11(1): p. 1092-1109.  
  107. Ahmadi, M., Iron oxide nanoparticles for delivery purposes, in Nanoengineered Biomaterials for Advanced Drug Delivery. 2020, Elsevier. p. 373-393.  
  108. Sathyanarayanan, M.B., et al., The effect of gold and iron-oxide nanoparticles on biofilm-forming pathogens. International Scholarly Research Notices, 2013. 2013.  
  109. Thakur, A., et al., Recent advancements in surface modification, characterization and functionalization for enhancing the biocompatibility and corrosion resistance of biomedical implants. Coatings, 2022. 12(10): p. 1459.  
  110. Kasthuri, G., A.N. Reddy, and P.M. Roopa, Application of green synthesized iron nanoparticles for enhanced antimicrobial activity of selected traditional and commonly exploited drug amoxicillin against Streptococcus mutans. Biosciences Biotechnology Research Asia, 2017. 14(3): p. 1135-1141.  
  111. Li, Z., et al., Facilely green synthesis of silver nanoparticles into bacterial cellulose. Cellulose, 2015. 22: p. 373-383.  
  112. Sharma, P., N. Bhardwaj, and V. Kumar, Swertia chirata extract mediated synthesis of iron oxide nanoparticles and its use as corrosion inhibitor for stainless steel 316 L in Ringer's solution. Advances in Natural Sciences: Nanoscience and Nanotechnology, 2021. 12(3): p. 035012.  
  113. Ramalingam, V., et al., Green fabrication of iron oxide nanoparticles using grey mangrove Avicennia marina for antibiofilm activity and in vitro toxicity. Surfaces and Interfaces, 2019. 15: p. 70-77.  
  114. Kaliamurthi, S., et al., The relationship between Chlorella sp. and zinc oxide nanoparticles: Changes in biochemical, oxygen evolution, and lipid production ability. Process Biochemistry, 2019. 85: p. 43-50.  
  115. Moradpoor, H., et al., An overview of recent progress in dental applications of zinc oxide nanoparticles. RSC advances, 2021. 11(34): p. 21189-21206.  
  116. Li, Y., et al., Enhancing ZnO-NP antibacterial and osteogenesis properties in orthopedic applications: A review. International journal of nanomedicine, 2020: p. 6247-6262.  
  117. Qu, J., et al., Zinc accumulation and synthesis of ZnO nanoparticles using Physalis alkekengi L. Environmental pollution, 2011. 159(7): p. 1783-1788.  
  118. Ting, B.Y.S., et al., Biosynthesis and Response of Zinc Oxide Nanoparticles against Periimplantitis Triggering Pathogens. Materials, 2022. 15(9): p. 3170.  
  119. Abdulkareem, E.H., et al., Anti-biofilm activity of zinc oxide and hydroxyapatite nanoparticles as dental implant coating materials. Journal of dentistry, 2015. 43(12): p. 1462-1469.  
  120. Nadeem, M., et al., The current trends in the green syntheses of titanium oxide nanoparticles and their applications. Green chemistry letters and reviews, 2018. 11(4): p. 492-502.  
  121. Dobrucka, R., Synthesis of titanium dioxide nanoparticles using Echinacea purpurea herba. Iranian journal of pharmaceutical research: IJPR, 2017. 16(2): p. 756.  
  122. Rajkumari, J., et al., Synthesis of titanium oxide nanoparticles using Aloe barbadensis mill and evaluation of its antibiofilm potential against Pseudomonas aeruginosa PAO1. Journal of Photochemistry and Photobiology B: Biology, 2019. 201: p. 111667.  
  123. Chowdhury, M.A., et al., Development of SiC-TiO2-Graphene neem extracted antimicrobial nano membrane for enhancement of multiphysical properties and future prospect in dental implant applications. Heliyon, 2022. 8(9): p. e10603.