Gold and Silver Nanoparticles with Modified Chitosan /PVA : Synthesis, Study The Toxicity and Anticancer Activity

Document Type : Original Research Article


Department of Chemistry, College of Education for Pure Science(Ibn Al-Haitham)/University of Baghdad, Iraq


The new novel polymers nanocomposites based modified chitosan (CS) blending with polyvinyl alcohol (PVA) and coated gold or silver nanoparticles (AuNPs), (AgNPs) were synthesized from many sequence reactions as presented in (Scheme1, 2 and 3). By utilizing 1H-NMR spectroscopy, FTIR, and Field Emission Scanning electron microscope , the synthesized compounds have been identified. Molecular docking is studied, where operations are used to predict the binding status of compounds with the enzyme and to calculate the free energy (ΔG) of the compounds prepared. Also, the antibacterial activity regarding the synthesized compounds against two resistant pathogenic bacteria (G+) S. aureus and E. coli (G-) was examined in vitro compare with standard antibiotic (Amoxicillin). The cytotoxic effect of novel polymers nanocomposites against Human Lung Adenocarcinoma Cells line (A549) using MTT assay was used to estimate and compare with normal cell line Rat Embryonic Fibroblasts (REF), the (modified chitosan/PVA/Au) exhibited very excellent inhibition rate. Finally, the Acute Toxicity Test of these nanocomposities were examined showed non-toxicity of these nanocomposities and histological examination of internal organs: liver, lung and kidney related to treated group showed no changes but similar those of control group.


Main Subjects

  1. 1.MicaelaTriunfo, ElenaTaf1, AnnaGuarnieri, Rosanna Salvia, Carmen Scieuzo, Thomas Hahn , Susanne Zibek, AlessandroGagliardini, Luca Panariello, Maria Beatrice Coltelli, Angela De Bonis and Patrizia Falabella, Characterization of chitin and chitosan derived from Hermetia illucens, a further step in a circular economy process, Scientifc Reports, 2022; 12(6613),

    1. Paula Sartori, Ana Paula Longaray Delamare , Giovanna Machado , Declan M. Devine , Janaina S. Crespo and Marcelo Giovanela, Synthesis and Characterization of Silver Nanoparticles for the Preparation of Chitosan Pellets and Their Application in Industrial Wastewater Disinfection,Water, 2023; 15(1), 190,  

    3.Amna H. Faid, Samia A. Shouman , Yehia A. Badr, Marwa Sharaky , Elham M. Mostafa and Mahmoud A. Sliem, Gold nanoparticles loaded chitosan encapsulate 6-mercaptopurine as a novel nanocomposite for chemo-photothermal therapy on breast cancer, BMC Chemistry, 2022; 16(94),  

    4.Mohaddeseh Kariminejad, Rezvan Zibaei, Azin Kolahdouz-Nasiri, Reza Mohammadi, Amir M. Mortazavian, Sara Sohrabvandi, Elham Khanniri, Nasim Khorshidian , Chitosan/Polyvinyl Alcohol/SiO2 Nanocomposite Films: Physicochemical and Structural Characterization, Biointerface Res. Appl. Chem, 2022; 12(3), 3725-3734.  

    1. Alexander Pestov, Yuliya Privar, Arseny Slobodyuk, Andrey Boroda and Svetlana Bratskaya, Chitosan Cross-Linking with Acetaldehyde Acetals, Biomimetics, 2022; 7(1),1-10.  
    2. Ali. A.Taha, Nahida.J.Hameed and Farah.H. Rashid, Preparation and Characterization of (Hyacinth plant / Chitosan) Composite as a Heavy Metal Removal, Baghdad Sci. J, 2019; 16(4),865-870.  
    3. Hitesh Chopra, Shabana Bibi, Sandeep Kumar, Muhammad Saad Khan, Pradeep Kumar and Inderbir Singh , Preparation and Evaluation of Chitosan/PVA Based Hydrogel Films Loaded with Honey for Wound Healing Application, Gels, 2022; 8(2),111.  
    4. Ahmed A.Ounab, Gye HwaShinc, Jun TaeKima , Antimicrobial, antioxidant, and pH-sensitive polyvinyl alcohol/chitosan-based composite films with aronia extract, cellulose nanocrystals, and grapefruit seed extract, Int J Biol Macromol., 2022; 213, 381-393.  
    5. Ali A. Taha, Nahida J. Hameed and Farah H. Rashid, Decolorization of Phenol Red Dye by Immobilized Laccase in Chitosan Beads Using Laccase -Mediator -System Model, Baghdad Sci. J., 2020; 17(3), 720-725.  
    6. Bianca-Elena-Beatrice Cretu, Loredana Elena Nita, Alexandru-Mihail S, erban, Alina Gabriela Rusu, Florica Doroftei and Aurica P. Chiriac, New Cryogels Based on Poly(vinyl alcohol) and a Copolymacrolactone System: I-Synthesis and Characterization, Nanomaterials 2022, 12, 2420.  

    11.Diana Serbezeanu , Tăchit,ă Vlad-Bubulac, Mihaela Dorina Onofrei , Florica Doroftei , Corneliu Hamciuc , Alina-Mirela Ipate , Alexandru Anisiei , Gabriela Lisa, Ion Anghel , Ioana-Emilia ¸Sofran and Vasilica Popescu, Phosphorylated Poly(vinyl alcohol) Electrospun Mats for Protective Equipment Applications, Nanomaterials 2022, 12, 2685.  

    12.. Hanaa J. Alesa, Balqees M. Aldabbag and Rana M. Salih, Natural Pigment -Poly Vinyl Alcohol Nano composites Thin Films for Solar Cell, Baghdad Sci. J., 2020; 17(3):832-840  

    1. Duaa Akram, Nahida J. Hameed, Preparation and Characterization of PANI/PVA Blends as Electrolyte Materials, Journal of Applied Sciences and Nanotechnology, 2022; 2(2).  
    2. Baodong Liu , Jianhua Zhang and Hongge Gu, Research Progress of Polyvinyl Alcohol Water-Resistant Film Materials, Membranes 2022;12, 347.  

    15.XU, Ying SHI, Li GAO, Nan SHI, Junyan YANG, Rui HAO , Preparation and characterization of PH-responsive polyvinyl alcohol/chitosan/ anthocyanin films Hongyu Food Sci. Technol, Campinas, 2023; 43, e98022,  

    1. Haliza Katas, Noor Zianah Moden, Chei Sin Lim, Terence Celesistinus, Jie Yee Chan, Pavitra Ganasan, and Sundos Suleman Ismail Abdalla, Biosynthesis and Potential Applications of Silver and Gold Nanoparticles and Their Chitosan-Based Nanocomposites in Nanomedicine, Hindawi Journal of Nanotechnology, 2018; Article ID 4290705, 13 pages  
    2. Marco Laurence M. Budlayan, Jeanne Phyre L. Oracion , Lyka B. De La Rosa, Mikee Joy D. Rodriguez, Jonathan N. Patricio , Ser John Lynon P. Perez , Susan D. Arco, Jonathan P. Manigo, Eleanor S. Austria, Arnold C. Alguno, Custer C. Deocaris, Rey Y. Capangpangan, Preparation of Spin-Coated Poly(vinyl alcohol)/ chitosan/ Gold Nanoparticles Composite and Its Potential for Colorimetric Detection of Cyanide in Water, Pol. J. Environ. Stud. 2022;31(2), 1569-1576,  
    3. Francisco Javier Flórez -Barajas,Zayda Constanza Sánchez -Acevedo , Heriberto Peña -Pedraza, Synthesis and characterization of gold nanoparticles in solution using chitosan as reducing agent, Respuestas,2019; 24 (2), 49-55.  
    4. Leandro A. Almeida , Bruno V. M. Rodrigues, Debora T. Balogh , Rafaela. Sanfelice , Luiza A. Mercante , Amanda F. Frade-Barros and Adriana Pavinatto, Chitosan/Gold Nanoparticles Nanocomposite Film for Bisphenol A Electrochemical Sensing, Electrochem 2022; 3, 239-247.  
    5. Zhijun Guo, Dan Sun , Xian Zhou , Huan Xu , Yizhou Huang , Chenglin Chu and Baolong Shen, AuNP/Chitosan Nanocomposites Synthesized through Plasma Induced Liquid Chemistry and Their Applications in Photothermal Induced Bacteria Eradication, Pharmaceutics 2022; 14, 2147.  
    6. Amr H. Hashem , Amr M. Shehabeldine , Omar M. Ali and Salem S. Salem, Synthesis of Chitosan-Based Gold Nanoparticles: Antimicrobial and Wound-Healing Activities, Polymers 2022; 14, 2293.  
    7. Danmin Yang, Qun Liu, Yahui Gao, Shoumei Wan, Fanrong Meng, Wuyin Weng, Yucang Zhang, Characterization of silver nanoparticles loaded chitosan/polyvinyl alcohol antibacterial films for food packaging, Food Hydrocolloids, 2023;136, Part B, 108305,  
    8. Ali H. S., Khalid F. A., Ruwaidah S. S., Ibn Al-Haitham Jour. for Pure & Appl. Sci., 2014; 27 (3). 350-364.  
    9. RUWAIDAH S. SAEED, MUNA S. AL-RAWI , Synthesis, Characterization, Study the Toxicity and Anticancer Activity of N,O-Chitosan Derivatives, International Journal of Pharmaceutical Research, 2020; 12(2).  
    10. Shrba H.A., Hassan A.H., Ali F.K., Inter J. for Sci. and Technology, 2013; 8(2), 55-61.  

    26-Fouad M.S, Redha I., Al-Bagat, Araa Al -Juboori, Al- Mustansiriya J. Sci., 2006;17(3),15-26.  

    27.Zhimei Z., Bayaer A., Hui X. and Shuang Z., Structure and antimicrobial activities of benzoyl phenyl-thiosemicarbazone chitosan. International Journal of Biological Macromolecules, 2012; 50,1169-1174.  

    1. Li X., Goh S. H., Lai Y. H., & Wee A. T. S., Miscibility of carboxyl-containing polysiloxane/poly(vinylpyridine) blends, Polymer, 2000; 41(17), 6563-6571.  
    2. Hafeez H. Y., Lakhera S. K., Ashokkumar M., and Neppolian B., Ultrasound assisted synthesis of reduced graphene oxide (rGO) supported InVO4-TiO2 nanocomposite for efficient hydrogen production, Ultrason Sonochem. , 2019; 53, 1-10.  
    3. Badawy M.E.I.and Rabea E.I., African Journal of Microbiology Research, 2013;7(20), 2259- 2268.  
    4. Hiba, S.M; Sana,H.A. Synthesis And Characterization Of New Derivatives of Curcumin and Studying Their Biological Activities. IJDDT 2021, 11,(3)  
    5. Mohammed A. Almualla, Mazin N. Mousa, Mohammed Sattar, Chemical Modification and Characterization of Chitosan for Pharmaceutical Applications, Egypt. J. Chem., 2021; 64(7), 3635-3649.  
    6. Parul Sharma, Garima Mathur, Navendu Goswami, Sanjeev K. Sharma, Sanjay R. Dhakate, Subhash, et al., Evaluating the potential of chitosan/poly(vinyl alcohol) membranes as alternative carrier material for proliferation of Vero cells, e-Polymers, 2015; 15(4), 237-243  
    7. Holly Heaslet, Melissa Harris, Kelly Fahnoe, Ronald Sarver, Henry Putz, Jeanne Chang, Chakrapani Subramanyam, Gabriela BarreiroM and J. Richard Miller, Structural comparison of chromosomal and exogenous dihydrofolate reductase from Staphylococcus aureus in complex with the potent inhibitor trimethoprim, Proteins, 2009; 76, 706-717,  
    8. Omnia M. Abdallah, Khaled Z. EL-Baghdady, Mostafa M. H. Khalil, Mervat I. El Borhamy and Gamal A. Meligi, Antibacterial, antibiofilm and cytotoxic activities of biogenic polyvinyl alcohol-silver and chitosan-silver nanocomposites, Journal of Polymer Research 2020; 27(74),  
    9. Md. Amdadul Huq, Md. Ashrafudoulla , Md. Anowar Khasru Parvez , Sri Renukadevi Balusamy , Md. Mizanur Rahman , Ji Hyung Kim and Shahina Akter, Chitosan-Coated Polymeric Silver and Gold Nanoparticles: Biosynthesis, Characterization and Potential Antibacterial Applications: A Review, Polymers,2022; 14, 5302.  
    10. Kokila Thirupathi, Chaitany Jayaprakash Raorane , Vanaraj Ramkumar, Selvakumari Ulagesan , Madhappan Santhamoorthy , Vinit Raj, Gopal Shankar Krishnakumar, Thi Tuong Vy Phan and Seong-Cheol Kim , Update on Chitosan-Based Hydrogels: Preparation, Characterization, and Its Antimicrobial and Antibiofilm Applications, Gels,2023; 9, 35.  
    11. Sana H. Awad, Fatimah A. Hussein, Seenaa A. Mustafa,Synthesis, Characterization of Poly (chitosan nano-particles-co-pyruvic acid) and Substitution with Different Amino Acids, IJDDT, 2021 ; 11 (4).  
    12. Muna S. Al-Rawi, Synthesis of Some New Heterocyclic Compounds Via Chalcone Derivatives, Ibn Al-Haitham J. for Pure & Appl. Sci. 2015;28 (1).  
    13. Fayyadh Abed Nashaan, Muna Sameer Al-Rawi, Synthesis and Antimicrobial Activity of New 4-Fromyl Pyrazole Derivatives Drived from Galloyl Hydrazide, Chem. Methodol., 2023, 7(4) 267-276.  

    41.Freshney RI "Culture of Animal Cells: A manual of Basic Technique and Specialized Applications," 6th Edition, Wiley: New York,(2010).  

    42.Gao S., Ya BP., Dong WG. and Luo HS. "Ant proliferative effect of octreotide on gastric cancer cells mediated by inhibition of Akt/PKB and telomerase," World J. Gastroenterology. 2003; 9 (10), 2362- 2365  

    43.Ekaterina O. Mikhailova, J. Funct. Biomater. Gold Nanoparticles: Biosynthesis and Potential of Biomedical Application 2021; 12(4), 70;

    1. Rajaa, K. B.; Jameelah, K. T.; Huda, A. H.; Muna,S. Extraction, Purification of Alkaloid Compounds from Anastatica Hierochuntica and Studying its Effect on Liver Cancer Cells. International Journal of Pharmaceutical Research2020, 12(2)  

    45.Hanane M., Hindawi, Advance in pharmacological science, 2018: 3287193.  

    46.Aké-Assi E., N'guessan K. and Kouassi AF, European Scientific Journal, 2015;11 (27), 92- 100.