Isolation and characterization of curcumin by antisolvent and cooling crystallization method for a potential antimicrobial nanofibrous membrane

Document Type : Original Research Article


1 Institute of Biotechnology & Genetic Engineering, University of Sindh, Jamshoro 76080, Pakistan

2 Institute of Biotechnology and Genetic Engineering, University of sindh, Jamshoro

3 Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro 76080, Pakistan

4 Pakistan council of Scientific and Industrial Research Karachi laboratories complex Karachi

5 Institute of Pharmacy Shaheed Muhtarma Benazir Bhutto Medical University Larkana Sindh, Pakistan.


Objective(s): The main objective of this study is to develop a potential antibacterial nanofibrous membrane with a highly porous structure, and a large surface area to volume ratio from a synergistic combination of a synthetic polymer with a bioactive antimicrobial compound like curcumin for different biological applications like wound healing and food packing.
Methods: Soxhlet extraction and antisolvent cooling crystallization method were applied for the extraction of curcuminoids and curcumin. Characterization of isolated curcumin was carried out by FTIR spectroscopy and UV-spectrometry as validated according to the international conference of harmonization (ICH). The nanofibrous membrane was generated by an electrospinning technique from a synergistic mixture of polyvinyl alcohol with isolated curcumin.
Results: FTIR spectra confirm the presence of all the functional groups and UV-spectrophotometry presented total accuracy in % of 99.25 %, 99.56 %, 99.72 % and 99.96 % respectively. SEM results presented smooth, and continuous nanofibers without any bead-like structures with an average diameter of 215.38 + 29.32 nm in PVA-Cur-10 nanofibers samples. The antibacterial activity of isolated curcumin presented a 24.93+12.3 mm and 23.02 +1.2 mm zone of inhibition against S. aureus, and E.coli respectively.
Conclusions: This study presents the successful isolation of curcumin from crude curcuminoid by antisolvent and cooling crystallization method and its use in the preparation of a potential antibacterial electrospun nanofibrous membrane with PVA. The fabricated membrane exhibited excellent durability, strength and antibacterial properties, which can be used to protect wounds and food from harmful bacteria.


Main Subjects

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