Document Type : Original Research Article
Department of Chemistry, College of Science, University of Mustansiriyah, Baghdad, Iraq
Department of Chemistry, College of Education for Pure Science, University of Diyala, Baqubah, Diyala, Iraq
In this work, the eco-friendly synthesis of silveroxidenanoparticles (Ag2O-NPs) was successfully performed using orange leaf extract with silver nitrate and silver sulfate as precursors. Several techniques were used to characterize the surfaces of the silver oxide nanoparticles (Ag2O-NPs), including FT-IR spectra, which showed the functional groups present in the synthesis of silver oxide nanoparticles, FE-SEM images showed that the green synthesis of Ag2O-NO3-NPs and Ag2O-SO4-NPs has a spherical shape. EDX analysis spectrum confirmed the presence of elemental silver signals in the Ag2O-NPs, proving that the Ag2O-NPs were prepared within the nanoscale range and that there is a clear and tangible effect of negative ions bonded (NO3- and SO4-2) with Ag metal on the size, shape, and distribution of Ag2O-NPs. Also, the cytoxicity of the established Ag NPs at 0-200 µg/ml was assessed in breast cancer MCF-7 cell lines in vitro using MTT assay, and verified these strong cytotoxicity. As a result, the use of orange leaf extract is considered an eco-friendly and cost-effective method.